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Q4K7D7

- RUVC_PSEF5

UniProt

Q4K7D7 - RUVC_PSEF5

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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi139 – 1391MagnesiumUniRule annotation
Metal bindingi142 – 1421MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPFLU220664:GIX8-4806-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:PFL_4765
OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000008540: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 174174Crossover junction endodeoxyribonuclease RuvCPRO_0000225164Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi220664.PFL_4765.

Structurei

3D structure databases

ProteinModelPortaliQ4K7D7.
SMRiQ4K7D7. Positions 2-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiCIRTASD.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4K7D7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLILGIDPG SRITGYGVVR DTGRGCVYVA SGCIRTGAGE LPDRLQIVYR
60 70 80 90 100
GVREVIQTYG PVTMGIEKVF MARNADSALK LGQARGAAIV AGAEEGLEIA
110 120 130 140 150
EYTATQVKQA VAGTGGANKE QVQMMVMHLL KLTTKPQIDA SDALAIAICH
160 170
AHTRSSLLPH GLGTARSRGG RLRL
Length:174
Mass (Da):18,329
Last modified:August 2, 2005 - v1
Checksum:iC446386E20352A21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY93995.1.
RefSeqiYP_261846.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY93995; AAY93995; PFL_4765.
GeneIDi3479176.
KEGGipfl:PFL_4765.
PATRICi19878984. VBIPseFlu72549_4875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY93995.1 .
RefSeqi YP_261846.1. NC_004129.6.

3D structure databases

ProteinModelPortali Q4K7D7.
SMRi Q4K7D7. Positions 2-158.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 220664.PFL_4765.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY93995 ; AAY93995 ; PFL_4765 .
GeneIDi 3479176.
KEGGi pfl:PFL_4765.
PATRICi 19878984. VBIPseFlu72549_4875.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
KOi K01159.
OMAi CIRTASD.
OrthoDBi EOG6RG044.

Enzyme and pathway databases

BioCyci PFLU220664:GIX8-4806-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf-5 / ATCC BAA-477.

Entry informationi

Entry nameiRUVC_PSEF5
AccessioniPrimary (citable) accession number: Q4K7D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 2, 2005
Last modified: November 26, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3