Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q4K6J5 (Q4K6J5_PSEF5) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Synonyms:ddlB EMBL AAY94287.1
Ordered Locus Names:PFL_5059 EMBL AAY94287.1
OrganismPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP] EMBL AAY94287.1
Taxonomic identifier220664 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain116 – 311196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding142 – 19756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2651Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2781Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2781Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2801Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
Q4K6J5 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: A892F61AD33EEFCB

FASTA31833,883
        10         20         30         40         50         60 
MTAAYANLFS TVKPSDFGRV AVLYGGKSAE REVSLKSGKA VLEALQSGGV NAFGIDVGDD 

        70         80         90        100        110        120 
LLQRLLSEKI DRAFIILHGR GGEDGSMQGL LECLGIPYTG SGILASALAM DKLRTKQVWH 

       130        140        150        160        170        180 
SLGIPTPRHA VLSSEADCIS AVAELGLPLI VKPAHEGSSI GMAKVTSASE LIDAWKAAST 

       190        200        210        220        230        240 
YDSQVLVEQW IQGPEFTIAT LRDQVLPPIA LGTPHTFYDY DAKYLASDTQ YRIPCGLDSS 

       250        260        270        280        290        300 
KEQELMELTA KACEALGIAG WARADVMQDA QGQFWFLEVN TAPGMTDHSL VPMAARAAGL 

       310 
DFQQLVLAIL AASLEARG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000076 Genomic DNA. Translation: AAY94287.1.
RefSeqYP_262138.1. NC_004129.6.

3D structure databases

ProteinModelPortalQ4K6J5.
SMRQ4K6J5. Positions 18-311.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING220664.PFL_5059.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY94287; AAY94287; PFL_5059.
GeneID3479469.
KEGGpfl:PFL_5059.
PATRIC19879591. VBIPseFlu72549_5177.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycPFLU220664:GIX8-5099-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ4K6J5_PSEF5
AccessionPrimary (citable) accession number: Q4K6J5
Entry history
Integrated into UniProtKB/TrEMBL: August 2, 2005
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)