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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei567Aminoacyl-adenylateUniRule annotation1
Binding sitei611ATPUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPPRO220664:G1G4K-5489-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PFL_5321
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984461 – 943Isoleucine--tRNA ligaseAdd BLAST943

Proteomic databases

PRIDEiQ4K5U4

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_5321

Structurei

3D structure databases

ProteinModelPortaliQ4K5U4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi608 – 612"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q4K5U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK
60 70 80 90 100
FVLHDGPPYA NGSIHIGHAV NKILKDMIIR SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEVTH GKNLSSDQTR ELCRAYASEQ IEGQKAEFIR LGVLGDWANP
160 170 180 190 200
YKTMNFANEA GEIRALAEMV KGGFVFKGLK PVNWCFDCGS ALAEAEVEYQ
210 220 230 240 250
DKKSATIDVA FPVADEAKLA GAFGLAKLAK PAAIVIWTTT PWTIPANQAL
260 270 280 290 300
NVHPEFNYAL VDTGERLLVL AEELVESCLA RYNLEGSVIA TAPGSALELI
310 320 330 340 350
NFRHPFYDRL SPVYLAEYVE LGAGTGVVHS APAYGEDDFV TCKRYGMVND
360 370 380 390 400
DILNPVQSNG VYATSLEFFG GQFIWKANPA IVDKLSEVGA LLHTETISHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDKQPAAG DTLRQRALQA IEDTQFVPAW
460 470 480 490 500
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LNKESGDLHP RTVELMEEVA
510 520 530 540 550
QRVEQEGIEA WFKMDAAELL GDEAAQYDKI SDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHPMGHESGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF
610 620 630 640 650
TVDENGRKMS KSLGNVIAPQ KVNDTLGADI MRLWVSATDY SGEMAVSDQI
660 670 680 690 700
LQRSADAYRR IRNTARFLLS NLSGFNPATD LLPAEDMLAL DRWAVDRTLL
710 720 730 740 750
LQRELELHYG EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSKA
760 770 780 790 800
RRSAQTALYH ISEALVRWIA PILAFTADEL WEYLPGERNE SVMLNTWYEG
810 820 830 840 850
LTELPADFEL GREYWEGVMA VKVAVNKELE VQRAAKAVGG NLQAEVTLFA
860 870 880 890 900
EDGLAADLAK LSNELRFVLI TSTASLAPFA QAPADAVATE VPGLKLKVVK
910 920 930 940
SAFPKCARCW HCREDVGVHP EHPEICGRCV DNISGAGEVR HYA
Length:943
Mass (Da):105,158
Last modified:August 2, 2005 - v1
Checksum:i9D85035231BC03E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA Translation: AAY94531.1
RefSeqiWP_011063549.1, NC_004129.6

Genome annotation databases

EnsemblBacteriaiAAY94531; AAY94531; PFL_5321
KEGGipfl:PFL_5321
PATRICifig|220664.5.peg.5431

Similar proteinsi

Entry informationi

Entry nameiSYI_PSEF5
AccessioniPrimary (citable) accession number: Q4K5U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 2, 2005
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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