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Q4K5U4

- SYI_PSEF5

UniProt

Q4K5U4 - SYI_PSEF5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
    Binding sitei611 – 6111ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPFLU220664:GIX8-5362-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PFL_5321
    OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
    Taxonomic identifieri220664 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000008540: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 943943Isoleucine--tRNA ligasePRO_0000098446Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi220664.PFL_5321.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4K5U4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi608 – 6125"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4K5U4-1 [UniParc]FASTAAdd to Basket

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    MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK    50
    FVLHDGPPYA NGSIHIGHAV NKILKDMIIR SKTLAGFDAP YVPGWDCHGL 100
    PIEHKVEVTH GKNLSSDQTR ELCRAYASEQ IEGQKAEFIR LGVLGDWANP 150
    YKTMNFANEA GEIRALAEMV KGGFVFKGLK PVNWCFDCGS ALAEAEVEYQ 200
    DKKSATIDVA FPVADEAKLA GAFGLAKLAK PAAIVIWTTT PWTIPANQAL 250
    NVHPEFNYAL VDTGERLLVL AEELVESCLA RYNLEGSVIA TAPGSALELI 300
    NFRHPFYDRL SPVYLAEYVE LGAGTGVVHS APAYGEDDFV TCKRYGMVND 350
    DILNPVQSNG VYATSLEFFG GQFIWKANPA IVDKLSEVGA LLHTETISHS 400
    YMHCWRHKTP LIYRATAQWF VGMDKQPAAG DTLRQRALQA IEDTQFVPAW 450
    GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LNKESGDLHP RTVELMEEVA 500
    QRVEQEGIEA WFKMDAAELL GDEAAQYDKI SDTLDVWFDS GTTHWHVLRG 550
    SHPMGHESGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF 600
    TVDENGRKMS KSLGNVIAPQ KVNDTLGADI MRLWVSATDY SGEMAVSDQI 650
    LQRSADAYRR IRNTARFLLS NLSGFNPATD LLPAEDMLAL DRWAVDRTLL 700
    LQRELELHYG EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSKA 750
    RRSAQTALYH ISEALVRWIA PILAFTADEL WEYLPGERNE SVMLNTWYEG 800
    LTELPADFEL GREYWEGVMA VKVAVNKELE VQRAAKAVGG NLQAEVTLFA 850
    EDGLAADLAK LSNELRFVLI TSTASLAPFA QAPADAVATE VPGLKLKVVK 900
    SAFPKCARCW HCREDVGVHP EHPEICGRCV DNISGAGEVR HYA 943
    Length:943
    Mass (Da):105,158
    Last modified:August 2, 2005 - v1
    Checksum:i9D85035231BC03E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY94531.1.
    RefSeqiYP_262389.1. NC_004129.6.

    Genome annotation databases

    EnsemblBacteriaiAAY94531; AAY94531; PFL_5321.
    GeneIDi3479713.
    KEGGipfl:PFL_5321.
    PATRICi19880121. VBIPseFlu72549_5431.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY94531.1 .
    RefSeqi YP_262389.1. NC_004129.6.

    3D structure databases

    ProteinModelPortali Q4K5U4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 220664.PFL_5321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY94531 ; AAY94531 ; PFL_5321 .
    GeneIDi 3479713.
    KEGGi pfl:PFL_5321.
    PATRICi 19880121. VBIPseFlu72549_5431.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PFLU220664:GIX8-5362-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf-5 / ATCC BAA-477.

    Entry informationi

    Entry nameiSYI_PSEF5
    AccessioniPrimary (citable) accession number: Q4K5U4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3