Reviewed, UniProtKB/Swiss-Prot Q4K5R9 (GLYA1_PSEF5)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Ordered Locus Names:	PFL_5346

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000235002
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K5R9-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 65AB96E190E648E3	FASTA	417	44,892
	10 20 30 40 50 60 MFSRDLTIAK YDADLFAAME QEAQRQEEHI ELIASENYTS PAVMEAQGSV LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEY VDVVEQLAID RAKELFGADY ANVQPHAGSQ ANAAVYLALL QGGDTILGMS 130 140 150 160 170 180 LAHGGHLTHG AAVSSSGKLY NAIQYGIDAN GLIDYDEVER LAVEHKPKMI VAGFSAYSQI 190 200 210 220 230 240 LDFPRFREIA DKVGAYLFVD MAHVAGLVAA GVYPNPVPFA DVVTTTTHKT LRGPRGGLIL 250 260 270 280 290 300 ARANADIEKK LNSAVFPGAQ GGPLEHVIAA KAICFKEALQ PEFKAYQEQV VKNAQAMAEV 310 320 330 340 350 360 FIARGFDVVS GGTKNHLFLL SLIKQDISGK DADAALGKAF ITVNKNSVPN DPRSPFVTSG 370 380 390 400 410 LRFGTPAVTT RGFKEAECKE LAGWICDILA DLNNEAVIDA VREKVKAICK KLPVYGA

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY94556.1.
RefSeq	YP_262414.1.
3D structure databases
SMR	Q4K5R9. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K5R9.
Genome annotation databases
GeneID	3479738.
GenomeReviews	Gene locus PFL_5346 in contig CP000076_GR.
KEGG	pfl:PFL_5346.
NMPDR	fig|220664.3.peg.744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4K5R9.
OMA	DYEAVES.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5346-MON.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_PSEF5

Accession

Primary (citable) accession number: Q4K5R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	August 2, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)