Q4K5Q4 (RLMG_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	PFL_5361

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366476

Sequences

	Sequence		Length	Mass (Da)
	Q4K5Q4 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: FE3C362C0AECC547	FASTA	374	40,526
	10 20 30 40 50 60 MSLLASPFAQ LDLIRQPEQQ NEPLQAFDAA DEYLLNHLAE QQPASDTRVL VLNDSFGALA 70 80 90 100 110 120 ASLLGKVQVS SSGDSYLGAL ALEKNLVRNG LPFDAVRVVP ASEPLAGPFD RVLIRVPKTL 130 140 150 160 170 180 ALLEEQLIRL QGQLAPGAQV VAAAMVKHLP RAAGDLLERY IGPVQASLAV KKARLLIATP 190 200 210 220 230 240 QDKQPVVSPY PTRYRLEQPA IELLNHANVF CREGLDIGTR AFLPHLPQNL GSARVADLGC 250 260 270 280 290 300 GNGVLAIASA LDNPQAHYTL VDESFMAVQS ARENWQAVLG EREAQMRAGD GLAGQAAQSL 310 320 330 340 350 360 EVVLCNPPFH QQQVVGDFLA WRMFQQAREA LVVGGALYIV GNRHLGYHSK LARLFRGVEQ 370 VAATPKFVIL KARK

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY94571.1.
RefSeq	YP_262429.1. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4K5Q4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K5Q4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3479753.
GenomeReviews	Gene locus PFL_5361 in contig CP000076_GR.
KEGG	pfl:PFL_5361.
NMPDR	fig|220664.3.peg.759.
PATRIC	19880207. VBIPseFlu72549_5474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
PhylomeDB	Q4K5Q4.
ProtClustDB	CLSK868782.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5361-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEF5

Accession

Primary (citable) accession number: Q4K5Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program