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Q4K5H6 (SYL_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PFL_5439
OrganismPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]
Taxonomic identifier220664 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091347

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4K5H6 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 09C01DF96F163EAD

FASTA86896,954
        10         20         30         40         50         60 
MHEHYQPREI EAAAQLFWDE QKSFEVSEQP GKETYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMQG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW TYENIAYMKT QLRSLGLAVD 

       130        140        150        160        170        180 
WSREVTTCKP DYYRWEQWLF TRLFEKGVIY RKNGTVNWDP IDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALIEKREIPM YYFKITAYAD ELLESLDELP GWPEQVKTMQ RNWIGKSRGM EVQFPYDQAS 

       250        260        270        280        290        300 
IGEAGTLKVF TTRPDTLMGA TYVAVAAEHP LATLAAQDNP ELQAFIAECK GGSVAEADMA 

       310        320        330        340        350        360 
TQEKKGLPTS LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF EFATKYNLPI 

       370        380        390        400        410        420 
KAVVRTSAGD ETPAPWQDAY GEHGELINSG EFNGLDFPGA FDAIEVALLK KNLGQSRTQF 

       430        440        450        460        470        480 
RLRDWGISRQ RYWGCPIPII HCDSCGDVPV PQEQLPVVLP EDVVPDGAGS PLARMPEFYE 

       490        500        510        520        530        540 
CSCPKCGAPA KRETDTMDTF VESSWYYARY ASPHYEGGLV DPAAANHWLP VDQYIGGIEH 

       550        560        570        580        590        600 
AILHLLYARF FHKLMRDEGL VSSNEPFKNL LTQGMVIAET YYRREANGSY TWYNPADVEL 

       610        620        630        640        650        660 
ERDSKAKIIG AKLISDGLPV EIGGTEKMAK SKNNGVDPQS MIEQYGADTC RLFMMFASPP 

       670        680        690        700        710        720 
DMSLEWSDSG VEGAHRFLKR VWRLAQAHVS QGLPGALDIA TLSDEQKAIR RAIHLAIKQA 

       730        740        750        760        770        780 
SQDVGQHHKF NTAIAQVMTL MNVLEKAPQG SEQDRALLQE GLQTVALLLA PITPHISHEL 

       790        800        810        820        830        840 
WGQLGQSGAI IDAGWPALDE SALVQDSLQL VIQVNGKLRG QIEMPASASR EEVEAAARSN 

       850        860 
ENVLRFTEGL TIRKVIVVPG KLVNIVAS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000076 Genomic DNA. Translation: AAY94649.1.
RefSeqYP_262507.1. NC_004129.6.

3D structure databases

ProteinModelPortalQ4K5H6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING220664.PFL_5439.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY94649; AAY94649; PFL_5439.
GeneID3479831.
KEGGpfl:PFL_5439.
PATRIC19880363. VBIPseFlu72549_5552.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycPFLU220664:GIX8-5476-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSEF5
AccessionPrimary (citable) accession number: Q4K5H6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries