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Q4K5H6

- SYL_PSEF5

UniProt

Q4K5H6 - SYL_PSEF5

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei630 – 6301ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPFLU220664:GIX8-5476-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:PFL_5439
    OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
    Taxonomic identifieri220664 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000008540: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 868868Leucine--tRNA ligasePRO_1000091347Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi220664.PFL_5439.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4K5H6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi627 – 6315"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4K5H6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHEHYQPREI EAAAQLFWDE QKSFEVSEQP GKETYYCLSM FPYPSGKLHM    50
    GHVRNYTIGD VISRYQRMQG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW 100
    TYENIAYMKT QLRSLGLAVD WSREVTTCKP DYYRWEQWLF TRLFEKGVIY 150
    RKNGTVNWDP IDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAD 200
    ELLESLDELP GWPEQVKTMQ RNWIGKSRGM EVQFPYDQAS IGEAGTLKVF 250
    TTRPDTLMGA TYVAVAAEHP LATLAAQDNP ELQAFIAECK GGSVAEADMA 300
    TQEKKGLPTS LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF 350
    EFATKYNLPI KAVVRTSAGD ETPAPWQDAY GEHGELINSG EFNGLDFPGA 400
    FDAIEVALLK KNLGQSRTQF RLRDWGISRQ RYWGCPIPII HCDSCGDVPV 450
    PQEQLPVVLP EDVVPDGAGS PLARMPEFYE CSCPKCGAPA KRETDTMDTF 500
    VESSWYYARY ASPHYEGGLV DPAAANHWLP VDQYIGGIEH AILHLLYARF 550
    FHKLMRDEGL VSSNEPFKNL LTQGMVIAET YYRREANGSY TWYNPADVEL 600
    ERDSKAKIIG AKLISDGLPV EIGGTEKMAK SKNNGVDPQS MIEQYGADTC 650
    RLFMMFASPP DMSLEWSDSG VEGAHRFLKR VWRLAQAHVS QGLPGALDIA 700
    TLSDEQKAIR RAIHLAIKQA SQDVGQHHKF NTAIAQVMTL MNVLEKAPQG 750
    SEQDRALLQE GLQTVALLLA PITPHISHEL WGQLGQSGAI IDAGWPALDE 800
    SALVQDSLQL VIQVNGKLRG QIEMPASASR EEVEAAARSN ENVLRFTEGL 850
    TIRKVIVVPG KLVNIVAS 868
    Length:868
    Mass (Da):96,954
    Last modified:August 2, 2005 - v1
    Checksum:i09C01DF96F163EAD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY94649.1.
    RefSeqiYP_262507.1. NC_004129.6.

    Genome annotation databases

    EnsemblBacteriaiAAY94649; AAY94649; PFL_5439.
    GeneIDi3479831.
    KEGGipfl:PFL_5439.
    PATRICi19880363. VBIPseFlu72549_5552.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY94649.1 .
    RefSeqi YP_262507.1. NC_004129.6.

    3D structure databases

    ProteinModelPortali Q4K5H6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 220664.PFL_5439.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY94649 ; AAY94649 ; PFL_5439 .
    GeneIDi 3479831.
    KEGGi pfl:PFL_5439.
    PATRICi 19880363. VBIPseFlu72549_5552.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci PFLU220664:GIX8-5476-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf-5 / ATCC BAA-477.

    Entry informationi

    Entry nameiSYL_PSEF5
    AccessioniPrimary (citable) accession number: Q4K5H6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3