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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei630ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPRO220664:G1G4K-5604-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PFL_5439
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000913471 – 868Leucine--tRNA ligaseAdd BLAST868

Interactioni

Protein-protein interaction databases

STRINGi220664.PFL_5439

Structurei

3D structure databases

ProteinModelPortaliQ4K5H6
SMRiQ4K5H6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 52"HIGH" regionAdd BLAST11
Motifi627 – 631"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T Bacteria
COG0495 LUCA
HOGENOMiHOG000200747
KOiK01869
OMAiTFMVLAP
OrthoDBiPOG091H01JZ

Family and domain databases

Gene3Di3.40.50.6202 hits
3.90.740.101 hit
HAMAPiMF_00049_B Leu_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR002302 Leu-tRNA-ligase
IPR025709 Leu_tRNA-synth_edit
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740 PTHR43740, 1 hit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 2 hits
PF13603 tRNA-synt_1_2, 1 hit
PF09334 tRNA-synt_1g, 1 hit
PRINTSiPR00985 TRNASYNTHLEU
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00396 leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q4K5H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEHYQPREI EAAAQLFWDE QKSFEVSEQP GKETYYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMQG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW
110 120 130 140 150
TYENIAYMKT QLRSLGLAVD WSREVTTCKP DYYRWEQWLF TRLFEKGVIY
160 170 180 190 200
RKNGTVNWDP IDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAD
210 220 230 240 250
ELLESLDELP GWPEQVKTMQ RNWIGKSRGM EVQFPYDQAS IGEAGTLKVF
260 270 280 290 300
TTRPDTLMGA TYVAVAAEHP LATLAAQDNP ELQAFIAECK GGSVAEADMA
310 320 330 340 350
TQEKKGLPTS LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF
360 370 380 390 400
EFATKYNLPI KAVVRTSAGD ETPAPWQDAY GEHGELINSG EFNGLDFPGA
410 420 430 440 450
FDAIEVALLK KNLGQSRTQF RLRDWGISRQ RYWGCPIPII HCDSCGDVPV
460 470 480 490 500
PQEQLPVVLP EDVVPDGAGS PLARMPEFYE CSCPKCGAPA KRETDTMDTF
510 520 530 540 550
VESSWYYARY ASPHYEGGLV DPAAANHWLP VDQYIGGIEH AILHLLYARF
560 570 580 590 600
FHKLMRDEGL VSSNEPFKNL LTQGMVIAET YYRREANGSY TWYNPADVEL
610 620 630 640 650
ERDSKAKIIG AKLISDGLPV EIGGTEKMAK SKNNGVDPQS MIEQYGADTC
660 670 680 690 700
RLFMMFASPP DMSLEWSDSG VEGAHRFLKR VWRLAQAHVS QGLPGALDIA
710 720 730 740 750
TLSDEQKAIR RAIHLAIKQA SQDVGQHHKF NTAIAQVMTL MNVLEKAPQG
760 770 780 790 800
SEQDRALLQE GLQTVALLLA PITPHISHEL WGQLGQSGAI IDAGWPALDE
810 820 830 840 850
SALVQDSLQL VIQVNGKLRG QIEMPASASR EEVEAAARSN ENVLRFTEGL
860
TIRKVIVVPG KLVNIVAS
Length:868
Mass (Da):96,954
Last modified:August 2, 2005 - v1
Checksum:i09C01DF96F163EAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA Translation: AAY94649.1
RefSeqiWP_011063657.1, NC_004129.6

Genome annotation databases

EnsemblBacteriaiAAY94649; AAY94649; PFL_5439
KEGGipfl:PFL_5439
PATRICifig|220664.5.peg.5552

Similar proteinsi

Entry informationi

Entry nameiSYL_PSEF5
AccessioniPrimary (citable) accession number: Q4K5H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 2, 2005
Last modified: February 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome