Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Gamma-glutamyl phosphate reductase

Gene

proA

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.UniRule annotation

Catalytic activityi

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-HAMAP
  2. NADP binding Source: InterPro

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciPFLU220664:GIX8-5493-MONOMER.
UniPathwayiUPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl phosphate reductaseUniRule annotation (EC:1.2.1.41UniRule annotation)
Short name:
GPRUniRule annotation
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenaseUniRule annotation
Glutamyl-gamma-semialdehyde dehydrogenaseUniRule annotation
Short name:
GSA dehydrogenaseUniRule annotation
Gene namesi
Name:proAUniRule annotation
Ordered Locus Names:PFL_5456
OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000008540: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Gamma-glutamyl phosphate reductasePRO_0000230014Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi220664.PFL_5456.

Structurei

3D structure databases

ProteinModelPortaliQ4K5F9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-glutamyl phosphate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0014.
HOGENOMiHOG000246356.
KOiK00147.
OMAiCNAIETL.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR000965. G-glutamylP_reductase.
IPR012134. Glu-5-SA_DH.
[Graphical view]
PfamiPF00171. Aldedh. 2 hits.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4K5F9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESVLDYMT RLGRAARQAS RVIARATTAQ KNRALLAAAD ALDAARPELT
60 70 80 90 100
AANEQDLANG RANGLEPALL DRLALTPARI DEMIEGLRQV AKLPDPIGEI
110 120 130 140 150
RDMRYLPSGI QVGKMRVPLG VIGIIYESRP NVTIDAASLC LKSGNATILR
160 170 180 190 200
GGSEAIHSNR AIATCIQQGL AAADLPPHVV QVVETTDRAA VGALITMPEF
210 220 230 240 250
VDVIVPRGGK SLIERVSRDA KVPVIKHLDG VCHVYIDVAA DLDKAIRIAD
260 270 280 290 300
NAKTQRYAPC NTMETLLVHS AIAERVLPPL AAIYRDKGVE LRGCAQTRAL
310 320 330 340 350
LGAGVLEATE EDWRTEYTAP ILSIRVLDNL EQAIEHINTY GSHHTDSIVT
360 370 380 390 400
ENFSDARRFL NEVDSSSVMV NASTRFADGF EYGLGAEIGI STDKLHARGP
410 420
VGLEGLTSEK YVVFGDGHVR T
Length:421
Mass (Da):45,393
Last modified:April 4, 2006 - v2
Checksum:i96451F6DACB70E19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY94666.2.
RefSeqiYP_262524.2. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY94666; AAY94666; PFL_5456.
GeneIDi3479848.
KEGGipfl:PFL_5456.
PATRICi19880399. VBIPseFlu72549_5570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY94666.2.
RefSeqiYP_262524.2. NC_004129.6.

3D structure databases

ProteinModelPortaliQ4K5F9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220664.PFL_5456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY94666; AAY94666; PFL_5456.
GeneIDi3479848.
KEGGipfl:PFL_5456.
PATRICi19880399. VBIPseFlu72549_5570.

Phylogenomic databases

eggNOGiCOG0014.
HOGENOMiHOG000246356.
KOiK00147.
OMAiCNAIETL.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00360.
BioCyciPFLU220664:GIX8-5493-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR000965. G-glutamylP_reductase.
IPR012134. Glu-5-SA_DH.
[Graphical view]
PfamiPF00171. Aldedh. 2 hits.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf-5 / ATCC BAA-477.

Entry informationi

Entry nameiPROA_PSEF5
AccessioniPrimary (citable) accession number: Q4K5F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: January 7, 2015
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.