Reviewed, UniProtKB/Swiss-Prot Q4K4Z4 (GPH_PSEF5)

Names and origin

Protein names

Recommended name:
    Phosphoglycolate phosphatase
      Short name=PGPase
      Short name=PGP
    EC=3.1.3.18

Gene names

Ordered Locus Names:

PFL_5630

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the dephosphorylation of 2-phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress By similarity.
Catalytic activity	2-phosphoglycolate + H2O = glycolate + phosphate. HAMAP MF_00495
Pathway	Organic acid metabolism; glycolic acid biosynthesis; glycolic acid from 2-phosphoglycolic acid: step 1/1. HAMAP MF_00495
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. CbbY/cbbZ/gph/yieH family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Phosphoglycolate phosphatase HAMAP MF_00495		PRO_0000238166
Sites
	Active site	19	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K4Z4-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: F990F75669CF02AA	FASTA	272	29,893
	10 20 30 40 50 60 MSAFEQLFQG RLPRLVMFDL DGTLVDSVPD LAAAVDQMLL KLGRKPAGVE AVREWVGNGA 70 80 90 100 110 120 PMLVRRALAN SLEAQGVDDV EAEYALELFN TAYEDSHELT VVYPGARETL KWLHKQGVEM 130 140 150 160 170 180 ALITNKPERF VAPLLDQMKI GRYFRWIIGG DTLPQKKPDP AALFFVMKMA SVPASQSLFV 190 200 210 220 230 240 GDSRSDVLAA KAAGVKCVAL SYGYNHGRPI AEESPALVID NLRALIPGCL EPAAEITLPD 250 260 270 AVPSHSGNSI VVVTRKLWMK VIKALARWRW RA

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY94823.1.
RefSeq	YP_262689.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3480005.
GenomeReviews	Gene locus PFL_5630 in contig CP000076_GR.
KEGG	pfl:PFL_5630.
NMPDR	fig|220664.3.peg.1200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4K4Z4.
OMA	Q4K4Z4. DSSNDAQ.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5630-MON.
Family and domain databases
HAMAP	MF_00495. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR006439. HAD-SF_hydro_IA_v1. IPR006402. HAD-SF_hydro_IA_v3. IPR005833. Haloacid_DH/epoxide_hydro. IPR006346. PGP_bact. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00413. HADHALOGNASE.
TIGRFAMs	TIGR01549. HAD-SF-IA-v1. 1 hit. TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01449. PGP_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPH_PSEF5

Accession

Primary (citable) accession number: Q4K4Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)