Reviewed, UniProtKB/Swiss-Prot Q4K4X3 (APAH_PSEF5)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
    EC=3.6.1.41
Alternative name(s):
    Diadenosine tetraphosphatase
    Ap4A hydrolase
    Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase

Gene names

Name:	apaH
Ordered Locus Names:	PFL_5651

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP By similarity.
Catalytic activity	P1,P(4)-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP. HAMAP MF_00199
Sequence similarities	Belongs to the Ap4A hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Bis(5'-nucleosyl)-tetraphosphatase, symmetrical HAMAP MF_00199		PRO_1000012078

Sequences

	Sequence		Length	Mass (Da)
	Q4K4X3-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: BB302E6FE829B4A8	FASTA	289	32,526
	10 20 30 40 50 60 MATYAVGDLQ GCLEPLQCLL EQVAFDPARD RLWLVGDLVN RGPQSLETLR YLYSIRDSLV 70 80 90 100 110 120 CVLGNHDLHL LAVWRNVERL KKSDTLREIL EAPDCEELLQ WLRQQKLMHY DEARNIAMVH 130 140 150 160 170 180 AGIAPQWSLK KAQKCAAEVE EALRDDNLFD PFLDGMYGND PAKWDSDLKG VTRLRVITNY 190 200 210 220 230 240 FTRMRFCTSD GKLDLKSKEG LDTAPPGYAP WFSHKERKTR DLKIIFGHWA ALEGHCNEPG 250 260 270 280 IFALDSGCVW GGAMTLLNVD SGVRLTCDCD AQGRTAALSP DPLPAPAKR

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY94844.1.
RefSeq	YP_262710.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3480026.
GenomeReviews	Gene locus PFL_5651 in contig CP000076_GR.
KEGG	pfl:PFL_5651.
NMPDR	fig|220664.3.peg.1221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4K4X3.
OMA	Q4K4X3. LVFGHWA.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5651-MON.
Family and domain databases
HAMAP	MF_00199. [Tree]
InterPro	IPR004617. ApaH. IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000903. B5n-ttraPtase_sm. 1 hit.
TIGRFAMs	TIGR00668. apaH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APAH_PSEF5

Accession

Primary (citable) accession number: Q4K4X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)