Q4K4X2 (GLPE_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase glpE
EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	PFL_5652

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity HAMAP MF_01009.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 109	109	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_1000062970
Regions
	Domain	16 – 104	89	Rhodanese
Sites
	Active site	64	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K4X2 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 47367507DB3B6044	FASTA	109	11,925
	10 20 30 40 50 60 MSEFKRIPPE QAQALREQGA VVVDIRDPQT YALNHISGSQ HLDNHSISDF IRAADLDAPL 70 80 90 100 VVVCYHGNSS QSAAAYLVSQ GFSDVYSLDG GFELWRSTYP AETAQGNPE

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY94845.1.
RefSeq	YP_262711.1. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4K4X2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K4X2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3480027.
GenomeReviews	Gene locus PFL_5652 in contig CP000076_GR.
KEGG	pfl:PFL_5652.
NMPDR	fig|220664.3.peg.1222.
PATRIC	19880805. VBIPseFlu72549_5764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0607.
HOGENOM	HBG743107.
OMA	AWHRHFP.
PhylomeDB	Q4K4X2.
ProtClustDB	PRK00162.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5652-MONOMER.
Family and domain databases
HAMAP	MF_01009. Thiosulf_sulfurtr. [Tree]
InterPro	IPR001763. Rhodanese-like. IPR023695. Thiosulf_sulfurTrfase. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
KO	K02439.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_PSEF5

Accession

Primary (citable) accession number: Q4K4X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program