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Q4K4S5

- MASZ_PSEF5

UniProt

Q4K4S5 - MASZ_PSEF5

Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi432 – 4321MagnesiumUniRule annotation
    Binding sitei432 – 4321GlyoxylateUniRule annotation
    Metal bindingi460 – 4601MagnesiumUniRule annotation
    Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei631 – 6311Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: JCVI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: JCVI

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPFLU220664:GIX8-5740-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:PFL_5700
    OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
    Taxonomic identifieri220664 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000008540: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Malate synthase GPRO_1000056919Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi220664.PFL_5700.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4K4S5.
    SMRiQ4K4S5. Positions 2-722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni457 – 4604Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiAWINAYE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4K4S5-1 [UniParc]FASTAAdd to Basket

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    MTEHVQVGGL QVARVLFDFV NNEAIPGTGL TADSFWAGAD KVIHDLAPKN    50
    KALLAKRDDF QARIDAWHQS RAGQAHDAVA YKAFLQDIGY LLPEAADFQA 100
    TTQNVDEEIA HMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE 150
    ADGAEKGKGY NKVRGDKVIA FARAFLDESA PLTAGSHVDS VSYKIVGGKL 200
    VVGLKGGSNS GLRNDAQLIG YQGDAAAPTA ILLKHNGLHF EIQIDATSPV 250
    GQTDAAGVKD LLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 300
    AEEVSKGGKS FTRTMNADRE YTGVNGEPVK LHGRSLLFVR NVGHLMTIDA 350
    ILDKDGNEVP EGILDGLVTS LASIHNLNGN TSRKNSRTGS MYIVKPKMHG 400
    PEEAAFTNEL FGRIEEVLNL PRNTLKVGIM DEERRTTVNL KACIKAASER 450
    VVFINTGFLD RTGDEIHTSM EAGAMVRKAD MKAEKWIGAY ENWNVDIGLS 500
    TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA 550
    LHYHKVDVFA RQAELAKRAR ASVDDILTIP LAVNPSWTPE QIKNELDNNA 600
    QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV 650
    VTQEQVLESL KRMAPVVDRQ NASDPLYRPL APDFDSNIAF QAALELVIEG 700
    TKQPNGYTEP VLHRRRREFK AKNGL 725
    Length:725
    Mass (Da):78,859
    Last modified:August 2, 2005 - v1
    Checksum:i85D274924CF2CD44
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY94890.1.
    RefSeqiYP_262758.1. NC_004129.6.

    Genome annotation databases

    EnsemblBacteriaiAAY94890; AAY94890; PFL_5700.
    GeneIDi3480072.
    KEGGipfl:PFL_5700.
    PATRICi19880901. VBIPseFlu72549_5811.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY94890.1 .
    RefSeqi YP_262758.1. NC_004129.6.

    3D structure databases

    ProteinModelPortali Q4K4S5.
    SMRi Q4K4S5. Positions 2-722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 220664.PFL_5700.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY94890 ; AAY94890 ; PFL_5700 .
    GeneIDi 3480072.
    KEGGi pfl:PFL_5700.
    PATRICi 19880901. VBIPseFlu72549_5811.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi AWINAYE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci PFLU220664:GIX8-5740-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf-5 / ATCC BAA-477.

    Entry informationi

    Entry nameiMASZ_PSEF5
    AccessioniPrimary (citable) accession number: Q4K4S5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3