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Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi432MagnesiumUniRule annotation1
Binding sitei432GlyoxylateUniRule annotation1
Metal bindingi460MagnesiumUniRule annotation1
Binding sitei541Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei631Proton donorUniRule annotation1

GO - Molecular functioni

  • malate synthase activity Source: JCVI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • glyoxylate cycle Source: UniProtKB-HAMAP
  • tricarboxylic acid cycle Source: JCVI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:PFL_5700
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569191 – 725Malate synthase GAdd BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei617Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiQ4K4S5.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_5700.

Structurei

3D structure databases

ProteinModelPortaliQ4K4S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni457 – 460Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4K4S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEHVQVGGL QVARVLFDFV NNEAIPGTGL TADSFWAGAD KVIHDLAPKN
60 70 80 90 100
KALLAKRDDF QARIDAWHQS RAGQAHDAVA YKAFLQDIGY LLPEAADFQA
110 120 130 140 150
TTQNVDEEIA HMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE
160 170 180 190 200
ADGAEKGKGY NKVRGDKVIA FARAFLDESA PLTAGSHVDS VSYKIVGGKL
210 220 230 240 250
VVGLKGGSNS GLRNDAQLIG YQGDAAAPTA ILLKHNGLHF EIQIDATSPV
260 270 280 290 300
GQTDAAGVKD LLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL
310 320 330 340 350
AEEVSKGGKS FTRTMNADRE YTGVNGEPVK LHGRSLLFVR NVGHLMTIDA
360 370 380 390 400
ILDKDGNEVP EGILDGLVTS LASIHNLNGN TSRKNSRTGS MYIVKPKMHG
410 420 430 440 450
PEEAAFTNEL FGRIEEVLNL PRNTLKVGIM DEERRTTVNL KACIKAASER
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGAMVRKAD MKAEKWIGAY ENWNVDIGLS
510 520 530 540 550
TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA
560 570 580 590 600
LHYHKVDVFA RQAELAKRAR ASVDDILTIP LAVNPSWTPE QIKNELDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV
660 670 680 690 700
VTQEQVLESL KRMAPVVDRQ NASDPLYRPL APDFDSNIAF QAALELVIEG
710 720
TKQPNGYTEP VLHRRRREFK AKNGL
Length:725
Mass (Da):78,859
Last modified:August 2, 2005 - v1
Checksum:i85D274924CF2CD44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY94890.1.
RefSeqiWP_011063875.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY94890; AAY94890; PFL_5700.
GeneIDi3480072.
KEGGipfl:PFL_5700.
PATRICi19880901. VBIPseFlu72549_5811.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY94890.1.
RefSeqiWP_011063875.1. NC_004129.6.

3D structure databases

ProteinModelPortaliQ4K4S5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220664.PFL_5700.

Proteomic databases

PRIDEiQ4K4S5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY94890; AAY94890; PFL_5700.
GeneIDi3480072.
KEGGipfl:PFL_5700.
PATRICi19880901. VBIPseFlu72549_5811.

Phylogenomic databases

eggNOGiCOG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_PSEF5
AccessioniPrimary (citable) accession number: Q4K4S5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 2, 2005
Last modified: November 30, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.