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Q4K4S5

- MASZ_PSEF5

UniProt

Q4K4S5 - MASZ_PSEF5

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Protein
Malate synthase G
Gene
glcB, PFL_5700
Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei276 – 2761Acetyl-CoA By similarity
Binding sitei313 – 3131Acetyl-CoA By similarity
Active sitei340 – 3401Proton acceptor By similarity
Binding sitei340 – 3401Glyoxylate By similarity
Metal bindingi432 – 4321Magnesium By similarity
Binding sitei432 – 4321Glyoxylate By similarity
Metal bindingi460 – 4601Magnesium By similarity
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei631 – 6311Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: JCVI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: JCVI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPFLU220664:GIX8-5740-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:PFL_5700
OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000008540: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase GUniRule annotation
PRO_1000056919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_5700.

Structurei

3D structure databases

ProteinModelPortaliQ4K4S5.
SMRiQ4K4S5. Positions 2-722.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA binding By similarity
Regioni457 – 4604Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiAWINAYE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4K4S5-1 [UniParc]FASTAAdd to Basket

« Hide

MTEHVQVGGL QVARVLFDFV NNEAIPGTGL TADSFWAGAD KVIHDLAPKN    50
KALLAKRDDF QARIDAWHQS RAGQAHDAVA YKAFLQDIGY LLPEAADFQA 100
TTQNVDEEIA HMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE 150
ADGAEKGKGY NKVRGDKVIA FARAFLDESA PLTAGSHVDS VSYKIVGGKL 200
VVGLKGGSNS GLRNDAQLIG YQGDAAAPTA ILLKHNGLHF EIQIDATSPV 250
GQTDAAGVKD LLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 300
AEEVSKGGKS FTRTMNADRE YTGVNGEPVK LHGRSLLFVR NVGHLMTIDA 350
ILDKDGNEVP EGILDGLVTS LASIHNLNGN TSRKNSRTGS MYIVKPKMHG 400
PEEAAFTNEL FGRIEEVLNL PRNTLKVGIM DEERRTTVNL KACIKAASER 450
VVFINTGFLD RTGDEIHTSM EAGAMVRKAD MKAEKWIGAY ENWNVDIGLS 500
TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA 550
LHYHKVDVFA RQAELAKRAR ASVDDILTIP LAVNPSWTPE QIKNELDNNA 600
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV 650
VTQEQVLESL KRMAPVVDRQ NASDPLYRPL APDFDSNIAF QAALELVIEG 700
TKQPNGYTEP VLHRRRREFK AKNGL 725
Length:725
Mass (Da):78,859
Last modified:August 2, 2005 - v1
Checksum:i85D274924CF2CD44
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY94890.1.
RefSeqiYP_262758.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY94890; AAY94890; PFL_5700.
GeneIDi3480072.
KEGGipfl:PFL_5700.
PATRICi19880901. VBIPseFlu72549_5811.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY94890.1 .
RefSeqi YP_262758.1. NC_004129.6.

3D structure databases

ProteinModelPortali Q4K4S5.
SMRi Q4K4S5. Positions 2-722.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 220664.PFL_5700.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY94890 ; AAY94890 ; PFL_5700 .
GeneIDi 3480072.
KEGGi pfl:PFL_5700.
PATRICi 19880901. VBIPseFlu72549_5811.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi AWINAYE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci PFLU220664:GIX8-5740-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf-5 / ATCC BAA-477.

Entry informationi

Entry nameiMASZ_PSEF5
AccessioniPrimary (citable) accession number: Q4K4S5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 2, 2005
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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