Reviewed, UniProtKB/Swiss-Prot Q4K4P6 (GLYA2_PSEF5)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	PFL_5729

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000235003
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K4P6-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 8B80852BED2DFE9D	FASTA	417	44,807
	10 20 30 40 50 60 MFSKQDQIQG YDDALLAAIN AEEQRQEDHI ELIASENYTS KRVMQAQGSG LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEH VDKVEALAIE RAKQLFGADY ANVQPHSGSS ANSEVYLALL QAGDTILGMS 130 140 150 160 170 180 LAHGGHLTHG AKVSSSGKLY NAVQYGIDTR TGLIDYDEVE RLAVEHKPKM IVAGFSAYSK 190 200 210 220 230 240 TLDFPRFRQI ADKVGALLFV DMAHVAGLVA AGLYPNPLPY ADVVTTTTHK TLRGPRGGLI 250 260 270 280 290 300 LAKANEEIEK KLNAAVFPGA QGGPLMHVIA AKAVCFKEAL EPGFKAYQQQ VIDNAQAMAG 310 320 330 340 350 360 VFIKRGYDVV SGGTDNHLFL VSLIRQGLTG KDADAALGRA HITVNKNAVP NDPQSPFVTS 370 380 390 400 410 GLRIGTPAVT TRGFKVTQCT ELAGWICDIL DHLGDADVEA NVARQVAALC ADFPVYR

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY94919.1.
RefSeq	YP_262787.1.
3D structure databases
SMR	Q4K4P6. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K4P6.
Genome annotation databases
GeneID	3480101.
GenomeReviews	Gene locus PFL_5729 in contig CP000076_GR.
KEGG	pfl:PFL_5729.
NMPDR	fig|220664.3.peg.1364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4K4P6.
OMA	QVIDNAQ.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5729-MON.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_PSEF5

Accession

Primary (citable) accession number: Q4K4P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	August 2, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)