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Q4K4C6

- TRMB_PSEF5

UniProt

Q4K4C6 - TRMB_PSEF5

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei71 – 711S-adenosyl-L-methionineUniRule annotation
    Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
    Binding sitei123 – 1231S-adenosyl-L-methionineUniRule annotation
    Active sitei146 – 1461By similarity
    Binding sitei146 – 1461S-adenosyl-L-methionineUniRule annotation
    Binding sitei150 – 1501SubstrateUniRule annotation
    Binding sitei182 – 1821SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPFLU220664:GIX8-5889-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:PFL_5849
    OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
    Taxonomic identifieri220664 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000008540: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 241241tRNA (guanine-N(7)-)-methyltransferasePRO_0000229185Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi220664.PFL_5849.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4K4C6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni219 – 2224Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4K4C6-1 [UniParc]FASTAAdd to Basket

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    MTESNDTPIQ PEAGDERQHR RIKSFVMRAG RMTEGQQRGL DQGAPLYVLP    50
    LADAPVDYDQ VFGRSAPRSL EIGFGMGHSL LEMAAAAPEQ DFIGVEVHRP 100
    GVGALLNGVL TQGLTNLRVY DCDAIEVLNR CIADNSLDRL MLFFPDPWHK 150
    SRHHKRRIVQ ASFAELVRSK LKVGGILHMA TDWEPYAEYM LEVMNVAPGY 200
    RNLAEDGKCV PRPAERPITK FERRGERLGH GVWDLKFEKL A 241
    Length:241
    Mass (Da):27,118
    Last modified:August 2, 2005 - v1
    Checksum:i61964E573D098FF7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY95039.1.
    RefSeqiYP_262907.1. NC_004129.6.

    Genome annotation databases

    EnsemblBacteriaiAAY95039; AAY95039; PFL_5849.
    GeneIDi3480221.
    KEGGipfl:PFL_5849.
    PATRICi19881205. VBIPseFlu72549_5963.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY95039.1 .
    RefSeqi YP_262907.1. NC_004129.6.

    3D structure databases

    ProteinModelPortali Q4K4C6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 220664.PFL_5849.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY95039 ; AAY95039 ; PFL_5849 .
    GeneIDi 3480221.
    KEGGi pfl:PFL_5849.
    PATRICi 19881205. VBIPseFlu72549_5963.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci PFLU220664:GIX8-5889-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf-5 / ATCC BAA-477.

    Entry informationi

    Entry nameiTRMB_PSEF5
    AccessioniPrimary (citable) accession number: Q4K4C6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3