Reviewed, UniProtKB/Swiss-Prot Q4K434 (ARGA_PSEF5)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	PFL_5941

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity HAMAP MF_01105.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084817
Regions
	Domain	286 – 425	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	Q4K434-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 685EEAB2C2D5B927	FASTA	432	47,675
	10 20 30 40 50 60 MPEYVNWLRH ASPYINAHRD CTFVVMLPGD GVEHPNFGNI VHDLVLLHSL GVRLVLVHGS 70 80 90 100 110 120 RPQIEARLAA RGLTPHYHHG LRITDAATLE CVIDAVGQLR IAIEARLSMD MASSPMQGSR 130 140 150 160 170 180 LRVASGNLVT ARPIGVLEGV DYHHTGEVRR VDRKGINRLL DERSIVLLSP LGYSPTGETF 190 200 210 220 230 240 NLACEDVATR AAIDLGADKL LLFGADPGLL DENGKLVREL RPQQVPAHLQ RLGGNYQAEL 250 260 270 280 290 300 LDAAAEACRG GVGRSHIVSY AEDGALLTEL FTRDGGGTLV AQEQFERVRE AAIEDVGGLL 310 320 330 340 350 360 ELISPLEEQG ILVRRSREVL EREIEQFSVV EREGMIIACA ALYQIADSDA GELACLAVNP 370 380 390 400 410 420 EYRHGKRGDE LLERIETRAR AQGLKTLFVL TTRTAHWFRE RGFVPSSVER LPSARASLYN 430 YQRNSKIFEK AL

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY95131.1.
RefSeq	YP_262999.1.
3D structure databases
HSSP	HSSP built from PDB template 2BUF based on UniProtKB Q9HTN2.
SMR	Q4K434. Positions 2-432.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K434.
Genome annotation databases
GeneID	3480313.
GenomeReviews	Gene locus PFL_5941 in contig CP000076_GR.
KEGG	pfl:PFL_5941.
NMPDR	fig|220664.3.peg.1134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG545264.
OMA	APYIHAF.
PhylomeDB	Q4K434.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5941-MONOMER.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSEF5

Accession

Primary (citable) accession number: Q4K434

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)