ID   GCSP2_PSEF5             Reviewed;         954 AA.
AC   Q4K416;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=Glycine dehydrogenase [decarboxylating] 2;
DE            EC=1.4.4.2;
DE   AltName: Full=Glycine decarboxylase 2;
DE   AltName: Full=Glycine cleavage system P-protein 2;
GN   Name=gcvP2; OrderedLocusNames=PFL_5959;
OS   Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A.,
RA   Rosovitz M.J., Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W.,
RA   Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A.,
RA   Pierson L.S. III, Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas
RT   fluorescens Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine. The P protein binds the alpha-amino group of glycine
CC       through its pyridoxal phosphate cofactor; CO(2) is released and
CC       the remaining methylamine moiety is then transferred to the
CC       lipoamide cofactor of the H protein (By similarity).
CC   -!- CATALYTIC ACTIVITY: Glycine + H-protein-lipoyllysine = H-protein-
CC       S-aminomethyldihydrolipoyllysine + CO(2).
CC   -!- COFACTOR: Pyridoxal phosphate (By similarity).
CC   -!- SUBUNIT: The glycine cleavage system is composed of four proteins:
CC       P, T, L and H (By similarity).
CC   -!- SIMILARITY: Belongs to the gcvP family.
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DR   EMBL; CP000076; AAY95149.1; -; Genomic_DNA.
DR   RefSeq; YP_263017.1; -.
DR   GeneID; 3480331; -.
DR   GenomeReviews; CP000076_GR; PFL_5959.
DR   KEGG; pfl:PFL_5959; -.
DR   NMPDR; fig|220664.3.peg.1152; -.
DR   HOGENOM; Q4K416; -.
DR   OMA; Q4K416; PAVNRVD.
DR   BioCyc; PFLU220664:PFL_5959-MON; -.
DR   GO; GO:0004375; F:glycine dehydrogenase (decarboxylating) act...; IEA:EC.
DR   GO; GO:0016829; F:lyase activity; IEA:InterPro.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavag...; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00711; -; 1.
DR   InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR   InterPro; IPR003437; GDC-P.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major_sub1.
DR   Gene3D; G3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 2.
DR   PANTHER; PTHR11773; GDC-P; 1.
DR   Pfam; PF01212; Beta_elim_lyase; 1.
DR   Pfam; PF02347; GDC-P; 1.
DR   TIGRFAMs; TIGR00461; gcvP; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Oxidoreductase; Pyridoxal phosphate.
FT   CHAIN         1    954       Glycine dehydrogenase [decarboxylating]
FT                                2.
FT                                /FTId=PRO_0000227117.
FT   MOD_RES     704    704       N6-(pyridoxal phosphate)lysine (By
FT                                similarity).
SQ   SEQUENCE   954 AA;  103396 MW;  B5350A629DF79871 CRC64;
     MLSLSQLREP NAFLNRHLGP DAEEQQAMLA SLGLGSRAEL IEQTVPPGIR FNRALDLPPA
     LDEAAALARL KGYAGQNQVW TSLIGMGYHA TLTPTVILRN VLENPGWYTA YTPYQPEIAQ
     GRLEALLNFQ QMTIDLTGLD LANASLLDEA TAAAEAMALA KRVSKSSSNL FFVDEHCHPQ
     TVSVVRTRAE GFGFELVVGG VDELSGHQVF GALLQYPDTH GEIRDLRPLI DQLHAQQALA
     CVAADLLSLL LLTPPGELGA DVVLGSSQRF GVPMGYGGPH AAFFACRDDY KRAMPGRIIG
     VSKDARGQVA LRMALQTREQ HIRREKANSN ICTAQVLLAN IAGFYAVYHG PAGLKRIAQR
     VHRLTCILAV GLERHGIARV NRHFFDTLTL EVGGSQTAII ESARAQQINL RILGRGRLGL
     SLDETCDEST VTRLFDVFLG ADHGLDVSNL DAEALESGIP DPLLRRTRYL THPVFSAHHS
     ETEMLRYLKQ LENKDLALNQ SMIPLGSCTM KLNASSEMIP ITWPEFANLH PFAPREQAAG
     YGLLIAELER WLCAITGFDA ICMQPNSGAQ GEYAGLLAIR RYHESRRQGG RHVCLIPASA
     HGTNPASAQM AGMQVVIVEC DEAGNVDLED LKAKAQAAGE RLSCLMATYP STHGVYEEGI
     SQICEVIHSH GGQVYMDGAN LNAQVGLARP ADIGADVSHM NLHKTFCIPH GGGGPGMGPI
     GVRAHLAPFV ANHPVVPIDG PLPENGAVSA APWGSASILP ISWMYIALMG PQLADASEVA
     ILAANYLAEQ LSGAFPVLYS GRNGRVAHEC ILDLRPLKAQ TGISEEDVAK RLMDYGFHAP
     TMSFPVPGTL MVEPTESESK AELDRFIEAM LSIRAEIAQV QEGNWPAEDN PLKGAPHTLA
     DITGVWERSY SIEQAVLPTA HTRAHKYWPA VNRVDNVYGD RNLFCACVPL ADYR
//