Q4K416 (GCSP2_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine dehydrogenase [decarboxylating] 2
EC=1.4.4.2

Alternative name(s):
Glycine cleavage system P-protein 2
Glycine decarboxylase 2

Gene names

Name:	gcvP2
Ordered Locus Names:	PFL_5959

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	954 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP MF_00711
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00711
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00711
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvP family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 954	954	Glycine dehydrogenase [decarboxylating] 2 HAMAP MF_00711		PRO_0000227117
Amino acid modifications
	Modified residue	704	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K416 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: B5350A629DF79871	FASTA	954	103,396
	10 20 30 40 50 60 MLSLSQLREP NAFLNRHLGP DAEEQQAMLA SLGLGSRAEL IEQTVPPGIR FNRALDLPPA 70 80 90 100 110 120 LDEAAALARL KGYAGQNQVW TSLIGMGYHA TLTPTVILRN VLENPGWYTA YTPYQPEIAQ 130 140 150 160 170 180 GRLEALLNFQ QMTIDLTGLD LANASLLDEA TAAAEAMALA KRVSKSSSNL FFVDEHCHPQ 190 200 210 220 230 240 TVSVVRTRAE GFGFELVVGG VDELSGHQVF GALLQYPDTH GEIRDLRPLI DQLHAQQALA 250 260 270 280 290 300 CVAADLLSLL LLTPPGELGA DVVLGSSQRF GVPMGYGGPH AAFFACRDDY KRAMPGRIIG 310 320 330 340 350 360 VSKDARGQVA LRMALQTREQ HIRREKANSN ICTAQVLLAN IAGFYAVYHG PAGLKRIAQR 370 380 390 400 410 420 VHRLTCILAV GLERHGIARV NRHFFDTLTL EVGGSQTAII ESARAQQINL RILGRGRLGL 430 440 450 460 470 480 SLDETCDEST VTRLFDVFLG ADHGLDVSNL DAEALESGIP DPLLRRTRYL THPVFSAHHS 490 500 510 520 530 540 ETEMLRYLKQ LENKDLALNQ SMIPLGSCTM KLNASSEMIP ITWPEFANLH PFAPREQAAG 550 560 570 580 590 600 YGLLIAELER WLCAITGFDA ICMQPNSGAQ GEYAGLLAIR RYHESRRQGG RHVCLIPASA 610 620 630 640 650 660 HGTNPASAQM AGMQVVIVEC DEAGNVDLED LKAKAQAAGE RLSCLMATYP STHGVYEEGI 670 680 690 700 710 720 SQICEVIHSH GGQVYMDGAN LNAQVGLARP ADIGADVSHM NLHKTFCIPH GGGGPGMGPI 730 740 750 760 770 780 GVRAHLAPFV ANHPVVPIDG PLPENGAVSA APWGSASILP ISWMYIALMG PQLADASEVA 790 800 810 820 830 840 ILAANYLAEQ LSGAFPVLYS GRNGRVAHEC ILDLRPLKAQ TGISEEDVAK RLMDYGFHAP 850 860 870 880 890 900 TMSFPVPGTL MVEPTESESK AELDRFIEAM LSIRAEIAQV QEGNWPAEDN PLKGAPHTLA 910 920 930 940 950 DITGVWERSY SIEQAVLPTA HTRAHKYWPA VNRVDNVYGD RNLFCACVPL ADYR

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY95149.1.
RefSeq	YP_263017.2. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4K416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K416.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3480331.
GenomeReviews	Gene locus PFL_5959 in contig CP000076_GR.
KEGG	pfl:PFL_5959.
NMPDR	fig|220664.3.peg.1152.
PATRIC	19881431. VBIPseFlu72549_6076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1003.
HOGENOM	HBG286723.
OMA	AMMGPQL.
PhylomeDB	Q4K416.
ProtClustDB	PRK12566.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_5959-MONOMER.
Family and domain databases
HAMAP	MF_00711. GcvP. [Tree]
InterPro	IPR020580. GDC-P_N. IPR020581. GDC_P. IPR003437. GDC_P_homo. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 2 hits.
KO	K00281.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 2 hits. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 2 hits.
TIGRFAMs	TIGR00461. GcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSP2_PSEF5

Accession

Primary (citable) accession number: Q4K416

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program