Q4K3U3 (XPT_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xanthine phosphoribosyltransferase
Short name=XPRTase
EC=2.4.2.22

Gene names

Name:	xpt
Ordered Locus Names:	PFL_6032

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas protegens

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts the preformed base xanthine, a product of nucleic acid breakdown, to xanthosine 5'-monophosphate (XMP), so it can be reused for RNA or DNA synthesis By similarity. HAMAP-Rule MF_01184
Catalytic activity	XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine. HAMAP-Rule MF_01184
Pathway	Purine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1. HAMAP-Rule MF_01184
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01184
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01184.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. Xpt subfamily.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	XMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleoside salvage Inferred from electronic annotation. Source: HAMAP xanthine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	xanthine phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Xanthine phosphoribosyltransferase HAMAP-Rule MF_01184		PRO_0000339731
Regions
	Region	128 – 132	5	5-phospho-alpha-D-ribose 1-diphosphate binding By similarity
Sites
	Binding site	20	1	Xanthine; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	27	1	Xanthine By similarity
	Binding site	156	1	Xanthine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K3U3 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 69D20A603D8FACBB	FASTA	190	20,404
	10 20 30 40 50 60 MEALHKKIRE EGIVLSDQVL KVDAFLNHQI DPQLMKLIGD EFATLFKDSG ITKIVTIEAS 70 80 90 100 110 120 GIAPAIMTGL NLGVPVIFAR KHQSLTLTEN LLSATVYSFT KQVESTVAIS PRHLTSSDRV 130 140 150 160 170 180 LIIDDFLANG KASQALISII KQAGATVAGL GIVIEKSFQG GRAELDSQGY RVESLARVKS 190 LAGGVVTFIE

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY95221.1.
RefSeq	YP_263090.1. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4K3U3.
SMR	Q4K3U3. Positions 1-190.
ModBase	Search...
Protein-protein interaction databases
STRING	220664.PFL_6032.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY95221; AAY95221; PFL_6032.
GeneID	3480403.
KEGG	pfl:PFL_6032.
PATRIC	19881595. VBIPseFlu72549_6158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0503.
HOGENOM	HOG000036777.
KO	K03816.
OMA	ATVYSFT.
ProtClustDB	PRK09219.
Enzyme and pathway databases
BioCyc	PFLU220664:GIX8-6076-MONOMER.
UniPathway	UPA00602; UER00658.
Family and domain databases
HAMAP	MF_01184. XPRTase.
InterPro	IPR000836. PRibTrfase_dom. IPR010079. Xanthine_PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01744. XPRTase. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XPT_PSEF5

Accession

Primary (citable) accession number: Q4K3U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	August 2, 2005
Last modified:	May 29, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program