Reviewed, UniProtKB/Swiss-Prot Q4K3F6 (PDXY_PSEF5)

Names and origin

Protein names

Recommended name:
    Pyridoxamine kinase
      Short name=PM kinase
    EC=2.7.1.35

Gene names

Name:	pdxY
Ordered Locus Names:	PFL_6169

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxamine By similarity.
Catalytic activity	ATP + pyridoxal = ADP + pyridoxal 5'-phosphate. HAMAP MF_01639
Sequence similarities	Belongs to the pyridoxine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from electronic annotation. Source: HAMAP pyridoxal kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Pyridoxamine kinase HAMAP MF_01639		PRO_0000269819
Regions
	Nucleotide binding	184 – 185	2	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	47	1	Substrate By similarity
	Binding site	225	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K3F6-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: A86B14491A019730	FASTA	290	31,264
	10 20 30 40 50 60 MKRTPHLLAI QSHVVFGHAG NSAAVFPMQR VGVNVWPLNT VQFSNHTQYG QWAGEVLAPQ 70 80 90 100 110 120 QIPELVEGIA AIGELGNCDA VLSGYLGSAA QGRAILSGVA RIKAVNPKAL YLCDPVMGHP 130 140 150 160 170 180 EKGCSVPAEV SDFLLDEAVA MADFLCPNQL ELDSFCGRKP QSLFDCLGMA RSLLDKGPRA 190 200 210 220 230 240 VLVKHLDYPG KLVDGFEMLL VTADGSWHLR RPLLAFPRQP VGVGDLTSGL FLARVLLGDS 250 260 270 280 290 LVAAFEFTAA AVHEVLLETQ ACASYELELV RAQDRIAHPR VRFEATPIGF

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY95357.1.
RefSeq	YP_263227.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3480539.
GenomeReviews	Gene locus PFL_6169 in contig CP000076_GR.
KEGG	pfl:PFL_6169.
NMPDR	fig|220664.3.peg.97.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4K3F6.
OMA	Q4K3F6. EVLLETQ.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_6169-MON.
Family and domain databases
HAMAP	MF_01639. [Tree]
InterPro	IPR004625. PyrdxlP_synth_PyrdxlKinase. [Graphical view]
TIGRFAMs	TIGR00687. pyridox_kin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXY_PSEF5

Accession

Primary (citable) accession number: Q4K3F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)