Q4K3E4 (HIS32_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosyl-AMP cyclohydrolase 2
Short name=PRA-CH 2
EC=3.5.4.19

Gene names

Name:	hisI2
Ordered Locus Names:	PFL_6181

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-AMP + H2O = 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide. HAMAP MF_01021
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9. HAMAP MF_01021
Subcellular location	Cytoplasm By similarity HAMAP MF_01021.
Sequence similarities	Belongs to the PRA-CH family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoribosyl-AMP cyclohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Phosphoribosyl-AMP cyclohydrolase 2 HAMAP MF_01021		PRO_0000229835

Sequences

	Sequence		Length	Mass (Da)
	Q4K3E4 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: E35D31123EE8DDF5	FASTA	147	16,205
	10 20 30 40 50 60 MIRRPPLSLL DLEAAPLGSR WALTAVLDAL PWNSDGLIAA IAQQHDSGEV LMLAWMNRQA 70 80 90 100 110 120 LGETLASGQA CYWSRSRRCL WRKGESSGHR QRLIEARLDC DGDAVLLLVD QQGPACHTGR 130 140 PNCFYNAIRD GAVEVISSPL KDSRHDS

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY95369.1.
RefSeq	YP_263239.1. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4K3E4.
SMR	Q4K3E4. Positions 29-141.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K3E4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3480551.
GenomeReviews	Gene locus PFL_6181 in contig CP000076_GR.
KEGG	pfl:PFL_6181.
NMPDR	fig|220664.3.peg.109.
PATRIC	19881899. VBIPseFlu72549_6310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0139.
HOGENOM	HBG294308.
OMA	GRRTCFH.
PhylomeDB	Q4K3E4.
ProtClustDB	CLSK2486446.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_6181-MONOMER.
Family and domain databases
HAMAP	MF_01021. HisI. [Tree]
InterPro	IPR002496. PRib_AMP_CycHydrolase. [Graphical view]
KO	K01496.
Pfam	PF01502. PRA-CH. 1 hit. [Graphical view]
ProDom	PD002610. PRA_CycHdrlase. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

HIS32_PSEF5

Accession

Primary (citable) accession number: Q4K3E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program