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Q4JY06 (TRMB_CORJK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:jk0149
OrganismCorynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]
Taxonomic identifier306537 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229162

Regions

Region233 – 2364Substrate binding By similarity

Sites

Active site1571 By similarity
Binding site821S-adenosyl-L-methionine By similarity
Binding site1071S-adenosyl-L-methionine By similarity
Binding site1341S-adenosyl-L-methionine By similarity
Binding site1571S-adenosyl-L-methionine By similarity
Binding site1611Substrate By similarity
Binding site1931Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4JY06 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: E95C69832F98E61C

FASTA25428,941
        10         20         30         40         50         60 
MVWMNNSENT PGSLPPGRPL QTEFNDGRDY PRLGNFSFRR GTLTDNQEAL WNEHWPKLGK 

        70         80         90        100        110        120 
VLADEVIDLP EWFDRDAETI AEIGSGTGTS TAAMAPLEAE KNIVAVELYR PGLAKLLGSV 

       130        140        150        160        170        180 
VRGDIHNVRM IRGDGVEVLQ RMFAPESLSG VRIYFPDPWP KARHHKRRIV QSGTLHLIAS 

       190        200        210        220        230        240 
RLKPGGILHI ATDHADYAEW IDELVEVEEQ LEYLGWPEDQ SDIPQLTDRQ VITKFEGKGL 

       250 
DKEHIIREYL WRKK 

« Hide

References

[1]"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K411.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR931997 Genomic DNA. Translation: CAI36301.1.
RefSeqYP_249919.1. NC_007164.1.

3D structure databases

ProteinModelPortalQ4JY06.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING306537.jk0149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI36301; CAI36301; jk0149.
GeneID3433515.
KEGGcjk:jk0149.
PATRIC21511160. VBICorJei31838_0162.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMANDGRDYP.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycCJEI306537:GJ8V-155-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CORJK
AccessionPrimary (citable) accession number: Q4JY06
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways