ID   PCKG_CORJK              Reviewed;         606 AA.
AC   Q4JY04;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   16-JUN-2009, entry version 28.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase [GTP];
DE            Short=PEP carboxykinase;
DE            Short=PEPCK;
DE            EC=4.1.1.32;
DE   AltName: Full=Phosphoenolpyruvate carboxylase;
GN   Name=pckG; Synonyms=pck; OrderedLocusNames=jk0151;
OS   Corynebacterium jeikeium (strain K411).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=306537;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15968079; DOI=10.1128/JB.187.13.4671-4682.2005;
RA   Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B.,
RA   Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T.,
RA   Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P.,
RA   Puehler A.;
RT   "Complete genome sequence and analysis of the multiresistant
RT   nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring
RT   bacterium of the human skin flora.";
RL   J. Bacteriol. 187:4671-4682(2005).
CC   -!- FUNCTION: Catalyzes the conversion of oxaloacetate (OAA) to
CC       phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic
CC       pathway that produces glucose from lactate and other precursors
CC       derived from the citric acid cycle (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + oxaloacetate = GDP + phosphoenolpyruvate
CC       + CO(2).
CC   -!- COFACTOR: Binds 1 manganese ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC       family.
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DR   EMBL; CR931997; CAI36303.1; -; Genomic_DNA.
DR   RefSeq; YP_249921.1; -.
DR   GeneID; 3432866; -.
DR   GenomeReviews; CR931997_GR; jk0151.
DR   KEGG; cjk:jk0151; -.
DR   NMPDR; fig|306537.3.peg.225; -.
DR   HOGENOM; Q4JY04; -.
DR   OMA; Q4JY04; NWFRMNE.
DR   BioCyc; CJEI306537:JK0151-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) act...; IEA:HAMAP.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:HAMAP.
DR   HAMAP; MF_00452; -; 1.
DR   InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008209; PEP_carboxykinase_GTP.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   Gene3D; G3DSA:3.90.228.20; PEP_carboxykinase_C; 1.
DR   Gene3D; G3DSA:3.40.449.10; PEP_carboxykinase_N; 1.
DR   PANTHER; PTHR11561; PEP_carboxykin; 1.
DR   Pfam; PF00821; PEPCK; 1.
DR   PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 1.
DR   ProDom; PD004738; PEPCK_N; 1.
DR   PROSITE; PS00505; PEPCK_GTP; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Decarboxylase; Gluconeogenesis;
KW   GTP-binding; Lyase; Manganese; Metal-binding; Nucleotide-binding.
FT   CHAIN         1    606       Phosphoenolpyruvate carboxykinase [GTP].
FT                                /FTId=PRO_0000103604.
FT   NP_BIND     270    275       GTP (By similarity).
FT   NP_BIND     512    515       GTP (By similarity).
FT   REGION      384    386       Substrate binding (By similarity).
FT   ACT_SITE    271    271       By similarity.
FT   METAL       227    227       Manganese (By similarity).
FT   METAL       247    247       Manganese (By similarity).
FT   METAL       294    294       Manganese (By similarity).
FT   BINDING      79     79       Substrate (By similarity).
FT   BINDING     220    220       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     227    227       Substrate (By similarity).
FT   BINDING     269    269       Substrate (By similarity).
FT   BINDING     386    386       GTP (By similarity).
FT   BINDING     417    417       GTP (By similarity).
SQ   SEQUENCE   606 AA;  67733 MW;  C4724EC230EC42A3 CRC64;
     MTIRGLVGEA PTKNQEMLNW IEEAVELFQP ESVVFCDGSE EEWNELAEQL VEHGTLIKLD
     EEAQPNSFLA RSNPSDVARV ESRTFICSKT EEDAGPTNNW MDPEQMRAEM REHFSGSMKG
     RRMYIVPFCM GPITDPSPKL GIEITDSPYV VMSMRIMTRM GKEALDKIGE NGEFVKGLHS
     VGAPLEPGQE DSTWPCNDTK YITHFPEDRE IWSYGSGYGG NAILAKKCYA LRIASAMARD
     EGWMAEHMLI LKLISPEDKA YYICAAFPSA CGKTNLAMIQ PTIPGWRAEV VGDDIAWLHF
     GEDGRLYAVN PENGFFGVAP GTNYASNPMA MKSMEPGNTL YTNVALTDDN NVWWESKEGE
     PQHLIDWLGN EWTPDSGNKA AHPNSRYCVP IEQCPVAAPE FNDPKGVPVS AILFGGRRAD
     TVPLVTQARD WNHATFIGAT LASGQTAAAA EAAVGSLRHD PMAMLPFIGY NAGDYLQHWI
     DMGNKGGDKM PEVFLVNWFR RGEDGRFLWP GFGENSRVLK WIIDRIEGRV EADETVVGYT
     ARYEDIYTDG LKETEEDIRE ALSVNPADWE RDLADNEEWL KFLGPKVPSE VWDEFQGLKD
     RVEAAK
//