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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateUniRule annotation1
Metal bindingi227ManganeseUniRule annotation1
Metal bindingi247Manganese; via tele nitrogenUniRule annotation1
Binding sitei269SubstrateUniRule annotation1
Active sitei271UniRule annotation1
Metal bindingi294ManganeseUniRule annotation1
Binding sitei386GTPUniRule annotation1
Binding sitei417GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi270 – 275GTPUniRule annotation6
Nucleotide bindingi512 – 515GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:jk0151
OrganismiCorynebacterium jeikeium (strain K411)
Taxonomic identifieri306537 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000545 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036041 – 606Phosphoenolpyruvate carboxykinase [GTP]Add BLAST606

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi306537.jk0151.

Structurei

3D structure databases

ProteinModelPortaliQ4JY04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 220Substrate bindingUniRule annotation3
Regioni384 – 386Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JY04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIRGLVGEA PTKNQEMLNW IEEAVELFQP ESVVFCDGSE EEWNELAEQL
60 70 80 90 100
VEHGTLIKLD EEAQPNSFLA RSNPSDVARV ESRTFICSKT EEDAGPTNNW
110 120 130 140 150
MDPEQMRAEM REHFSGSMKG RRMYIVPFCM GPITDPSPKL GIEITDSPYV
160 170 180 190 200
VMSMRIMTRM GKEALDKIGE NGEFVKGLHS VGAPLEPGQE DSTWPCNDTK
210 220 230 240 250
YITHFPEDRE IWSYGSGYGG NAILAKKCYA LRIASAMARD EGWMAEHMLI
260 270 280 290 300
LKLISPEDKA YYICAAFPSA CGKTNLAMIQ PTIPGWRAEV VGDDIAWLHF
310 320 330 340 350
GEDGRLYAVN PENGFFGVAP GTNYASNPMA MKSMEPGNTL YTNVALTDDN
360 370 380 390 400
NVWWESKEGE PQHLIDWLGN EWTPDSGNKA AHPNSRYCVP IEQCPVAAPE
410 420 430 440 450
FNDPKGVPVS AILFGGRRAD TVPLVTQARD WNHATFIGAT LASGQTAAAA
460 470 480 490 500
EAAVGSLRHD PMAMLPFIGY NAGDYLQHWI DMGNKGGDKM PEVFLVNWFR
510 520 530 540 550
RGEDGRFLWP GFGENSRVLK WIIDRIEGRV EADETVVGYT ARYEDIYTDG
560 570 580 590 600
LKETEEDIRE ALSVNPADWE RDLADNEEWL KFLGPKVPSE VWDEFQGLKD

RVEAAK
Length:606
Mass (Da):67,733
Last modified:August 2, 2005 - v1
Checksum:iC4724EC230EC42A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA. Translation: CAI36303.1.
RefSeqiWP_005297204.1. NC_007164.1.

Genome annotation databases

EnsemblBacteriaiCAI36303; CAI36303; jk0151.
GeneIDi3432866.
KEGGicjk:jk0151.
PATRICi21511164. VBICorJei31838_0164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA. Translation: CAI36303.1.
RefSeqiWP_005297204.1. NC_007164.1.

3D structure databases

ProteinModelPortaliQ4JY04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi306537.jk0151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI36303; CAI36303; jk0151.
GeneIDi3432866.
KEGGicjk:jk0151.
PATRICi21511164. VBICorJei31838_0164.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_CORJK
AccessioniPrimary (citable) accession number: Q4JY04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.