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Reviewed, UniProtKB/Swiss-Prot Q4JY04 (PCKG_CORJK)

Last modified June 16, 2009. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoenolpyruvate carboxykinase [GTP]
      Short name=PEP carboxykinase
      Short name=PEPCK
    EC=4.1.1.32
Alternative name(s):
    Phosphoenolpyruvate carboxylase
Gene names
Name: pckG
Synonyms: pck
Ordered Locus Names: jk0151
OrganismCorynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]
Taxonomic identifier306537 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

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Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity.

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

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Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452
PRO_0000103604

Regions

Nucleotide binding270 – 2756GTP By similarity
Nucleotide binding512 – 5154GTP By similarity
Region384 – 3863Substrate binding By similarity

Sites

Active site2711 By similarity
Metal binding2271Manganese By similarity
Metal binding2471Manganese By similarity
Metal binding2941Manganese By similarity
Binding site791Substrate By similarity
Binding site2201Substrate; via amide nitrogen By similarity
Binding site2271Substrate By similarity
Binding site2691Substrate By similarity
Binding site3861GTP By similarity
Binding site4171GTP By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q4JY04-1 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: C4724EC230EC42A3

FASTA60667,733
        10         20         30         40         50         60 
MTIRGLVGEA PTKNQEMLNW IEEAVELFQP ESVVFCDGSE EEWNELAEQL VEHGTLIKLD 

        70         80         90        100        110        120 
EEAQPNSFLA RSNPSDVARV ESRTFICSKT EEDAGPTNNW MDPEQMRAEM REHFSGSMKG 

       130        140        150        160        170        180 
RRMYIVPFCM GPITDPSPKL GIEITDSPYV VMSMRIMTRM GKEALDKIGE NGEFVKGLHS 

       190        200        210        220        230        240 
VGAPLEPGQE DSTWPCNDTK YITHFPEDRE IWSYGSGYGG NAILAKKCYA LRIASAMARD 

       250        260        270        280        290        300 
EGWMAEHMLI LKLISPEDKA YYICAAFPSA CGKTNLAMIQ PTIPGWRAEV VGDDIAWLHF 

       310        320        330        340        350        360 
GEDGRLYAVN PENGFFGVAP GTNYASNPMA MKSMEPGNTL YTNVALTDDN NVWWESKEGE 

       370        380        390        400        410        420 
PQHLIDWLGN EWTPDSGNKA AHPNSRYCVP IEQCPVAAPE FNDPKGVPVS AILFGGRRAD 

       430        440        450        460        470        480 
TVPLVTQARD WNHATFIGAT LASGQTAAAA EAAVGSLRHD PMAMLPFIGY NAGDYLQHWI 

       490        500        510        520        530        540 
DMGNKGGDKM PEVFLVNWFR RGEDGRFLWP GFGENSRVLK WIIDRIEGRV EADETVVGYT 

       550        560        570        580        590        600 
ARYEDIYTDG LKETEEDIRE ALSVNPADWE RDLADNEEWL KFLGPKVPSE VWDEFQGLKD 


RVEAAK

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References

[1]"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CR931997 Genomic DNA. Translation: CAI36303.1.
RefSeqYP_249921.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3432866.
GenomeReviewsGene locus jk0151 in contig CR931997_GR.
KEGGcjk:jk0151.
NMPDRfig|306537.3.peg.225.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ4JY04.
OMAQ4JY04. NWFRMNE.

Enzyme and pathway databases

BioCycCJEI306537:JK0151-MON.

Family and domain databases

HAMAPMF_00452.
[Tree]
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
PANTHERPTHR11561. PEP_carboxykin. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
ProDomPD004738. PEPCK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePCKG_CORJK
AccessionPrimary (citable) accession number: Q4JY04
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 2, 2005
Last modified: June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents