Q4JX59 (TYSY_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	jk0441

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Thymidylate synthase HAMAP MF_00008		PRO_0000321468
Sites
	Active site	156	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JX59 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 7FDE36C8BB7E56C6	FASTA	273	30,744
	10 20 30 40 50 60 MAPQQQPSSI PTPYEDLLRE ILETGTPKGD RTGTGTTSLF GRQIRYDLSA GFPLITTKSV 70 80 90 100 110 120 HVKSVVGELL WFLRGDSNVR WLQENGIRIW NEWADENGDL GPVYGVQWRS WPTPNGQHID 130 140 150 160 170 180 QIAGALETLQ TNPDSRRNIV SAWNVSELDN MALPPCHLLF QLYVANGKLS CQLYQRSADM 190 200 210 220 230 240 FLGVPFNIAS YSLLTHMFAQ QAGLEVGEFI WTGGDCHIYD NHVEQVKLQL SRDARPYPQL 250 260 270 KLNKAADMFS YDFSDIEFTG YDPHPVIKAK VAV

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI36598.1.
RefSeq	YP_250216.1. NC_007164.1.
3D structure databases
HSSP	HSSP built from PDB template 2G8O based on UniProtKB P0A884.
ProteinModelPortal	Q4JX59.
SMR	Q4JX59. Positions 14-273.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JX59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3433265.
GenomeReviews	Gene locus jk0441 in contig CR931997_GR.
KEGG	cjk:jk0441.
NMPDR	fig|306537.3.peg.1878.
PATRIC	21511764. VBICorJei31838_0453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	RMALAPC.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	CJEI306537:JK0441-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_CORJK

Accession

Primary (citable) accession number: Q4JX59

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program