Q4JWV0 (MDH_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	jk0548

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity. HAMAP MF_01517
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Malate dehydrogenase HAMAP MF_01517		PRO_0000113365
Regions
	Nucleotide binding	15 – 21	7	NAD By similarity
	Nucleotide binding	132 – 134	3	NAD By similarity
Sites
	Active site	190	1	Proton acceptor By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	108	1	NAD By similarity
	Binding site	115	1	NAD By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JWV0 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: F4DFB482B252F646	FASTA	330	35,064
	10 20 30 40 50 60 MTTNNQNAVT VTVTGAGGQI GYSLLFRIAK GEVFGDRVVN LRLLETEQGV QAARGVALEL 70 80 90 100 110 120 ADCAFPLLGE VSITTELSEG FKDANAVFLV GAKPRQKGEE RADLLAANGK IFGPQGKAIA 130 140 150 160 170 180 EYAADDVRVI VVGNPANTNA AIVAAHAEGL DPRRVTALTR LDHNRGLAQV ADKLGVAVRD 190 200 210 220 230 240 LKNMTVWGNH SASQFPDVAE LTLNGEKVAD KLDAAWVNDE FIPRVAKRGA EIIEVRGRSS 250 260 270 280 290 300 AASAASAALD HMRDWVNGTA EGDWVSVALP SDGSYGIPEG LVFSFPCRSV DGEWEIVQGL 310 320 330 EISPAQQERI DANIKELQEE KAAVEEAGLL

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI36707.1.
RefSeq	YP_250325.1. NC_007164.1.
3D structure databases
ProteinModelPortal	Q4JWV0.
SMR	Q4JWV0. Positions 7-330.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JWV0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3433195.
GenomeReviews	Gene locus jk0548 in contig CR931997_GR.
KEGG	cjk:jk0548.
NMPDR	fig|306537.3.peg.544.
PATRIC	21511990. VBICorJei31838_0560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG289884.
OMA	DPNVAFK.
ProtClustDB	PRK05442.
Enzyme and pathway databases
BioCyc	CJEI306537:JK0548-MONOMER.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. Divergent sequence. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_CORJK

Accession

Primary (citable) accession number: Q4JWV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program