Reviewed, UniProtKB/Swiss-Prot Q4JWU8 (SYV_CORJK)

Names and origin

Protein names

Recommended name:
    Valyl-tRNA synthetase
    EC=6.1.1.9
Alternative name(s):
    Valine--tRNA ligase
      Short name=ValRS

Gene names

Name:	valS
Ordered Locus Names:	jk0550

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	932 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity.
Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02004
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	valyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP valine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 932	932	Valyl-tRNA synthetase HAMAP MF_02004		PRO_0000224468
Regions
	Coiled coil	863 – 929	67	Potential
	Motif	75 – 85	11	"HIGH" region HAMAP MF_02004
	Motif	568 – 572	5	"KMSKS" region HAMAP MF_02004
Sites
	Binding site	571	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JWU8-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: E67C2E72F2147002	FASTA	932	104,813
	10 20 30 40 50 60 MPCLARLLDL ADVSDSSSKA NGSNPIGADR SAQLPAAWDP AAVEENLYQG WVDSGYFKAD 70 80 90 100 110 120 PSSDKPPFSI VLPPPNVTGQ LHMGHALDHT LMDAMARRKR MQGFEVLWLP GSDHAGIATQ 130 140 150 160 170 180 TKVEANLKET EGKDRFDYGR DAFVGKVWEW KDRYGGVIQR QMRAIGDSVD WSRERFTLDD 190 200 210 220 230 240 GLSRAVQTMF KELFDAGLIY RANRMVNWSP VLQTAISDIE VVYSDDEGEL VSIRYGSLED 250 260 270 280 290 300 SEPHVVVATT RVETMLGDVA VAVHPEDERY TDLVGKSLPH PFLPDRQMIV VADDYVDPEF 310 320 330 340 350 360 GTGAVKITPA HDPNDFAMGQ RHDLPMPVIM DETGHIANTG TEFDGMERYE AREKIRLALE 370 380 390 400 410 420 EQGRIVARKF PYVHSVGHSE RSKEAVEPRL SEQWFVKVEE LAKMSGDAIR SGDSVIHPSS 430 440 450 460 470 480 QEPRWFDWVD DMHDWCISRQ LWWGHRIPIW YGPNGEIVCC GPDDEAPTGE GWYQDEDVLD 490 500 510 520 530 540 TWFSSALWPF STMGWPEKTP ELEKFYPTSV LVTGYDILFF WVARMMMFAT FASKHTPEIL 550 560 570 580 590 600 GTGKDGRPQI PFNDIFLHGL VRDEHGRKMS KSLGNGIDPM DWVRDYGADA LRFTLARGAN 610 620 630 640 650 660 PGSDLPVGED AAQSSRNFAT KLYNATKFAL MNGARVGELP ARETLTDADR WILDRLEEVR 670 680 690 700 710 720 QLVDDALDRY EFSLANENLY RFAWGEFCDW YLEIAKVQIP RDWDSATEEQ VQRGIRTQIV 730 740 750 760 770 780 LGRVLDSVLR LLHPAMPFVT ETLWKALTDG EEGYSESLVT ADWPTADLTN GGAQTDADAV 790 800 810 820 830 840 RRMADVDKLV TELRRFRSDQ GVKPSQKVPA KLDFAAADLA NFEEAVRSLV RLETPEEDFA 850 860 870 880 890 900 ETASIEVRLS QATIAVQLDT SGTVDVAAER KRLEKDLAAA QKELDNAAKK LGNENFLAKA 910 920 930 PEKVVEGIRE RQRVAQEEFE RITARLEGLP KA

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR931997 Genomic DNA. Translation: CAI36709.1.
RefSeq	YP_250327.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3433539.
GenomeReviews	Gene locus jk0550 in contig CR931997_GR.
KEGG	cjk:jk0550.
NMPDR	fig|306537.3.peg.546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4JWU8.
OMA	Q4JWU8. TDQWYVS.
Enzyme and pathway databases
BioCyc	CJEI306537:JK0550-MON.
Family and domain databases
HAMAP	MF_02004. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR002303. Val-tRNA_synth_Ia. IPR019754. Val-tRNA_synth_Ia_N. IPR019499. Val-tRNA_synth_Ia_tRNA-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF5. tRNA-synt_val. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF10458. Val_tRNA-synt_C. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYV_CORJK

Accession

Primary (citable) accession number: Q4JWU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)