Q4JW85 (SYI_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	jk0760

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium jeikeium

Protein attributes

Sequence length	1092 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003
Cofactor	Zinc By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1092	1092	Isoleucine--tRNA ligase HAMAP-Rule MF_02003		PRO_0000098539
Regions
	Motif	61 – 71	11	"HIGH" region HAMAP-Rule MF_02003
	Motif	662 – 666	5	"KMSKS" region HAMAP-Rule MF_02003
Sites
	Binding site	665	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JW85 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: E9C5389841FBAAD6	FASTA	1,092	121,234
	10 20 30 40 50 60 MSEATPAGGA YPRVDMSNGG STAFPALEEN VLKYWEKDGT FNESVASREG NEEYVFYDGP 70 80 90 100 110 120 PFANGLPHYG HLLTGYVKDI VPRYQTMQGK KVARVFGWDC HGLPAELEAE KQLGIKDRAG 130 140 150 160 170 180 IEEMGMRSFN EYCATSVLRY TDEWKEYVTR QARWVDFDNG YKTMDPGFME SVMWAFKTLY 190 200 210 220 230 240 DKGLIYQGFR VLPYSWAEHT PLSNQETRLD DSYKMRQDPT ITVTFPITGA TEGTASVKTL 250 260 270 280 290 300 AEHPELADAA FIGWTTTPWT TPANLALAVN PEVTYAAVRI GDDAAGADGA EGMYGKTVVL 310 320 330 340 350 360 ADDLRGAYAK ELGEKAEVLG TFTGAELEGM TYEPLFGYFA ERAGEKASAG EGSEGGEGNQ 370 380 390 400 410 420 RKPGAFRVLC ADYVTTADGT GVVHQAPAFG EDDMFTCEAA GIDLEIPVDM DGKFTSLAPE 430 440 450 460 470 480 YQGQLVFDAN KAIIKDLKAA GRVLRHQTIE HSYPHSWRSG EPLIYMALPS WFVAVTKFRD 490 500 510 520 530 540 RMVELNHEQI EWMPSHIRDG QFGKWLEGAR DWNISRNRYW GAPIPVWVSD SEEYPRMDVY 550 560 570 580 590 600 GSLEELERDF GRKPASLHRP DIDELTRPNP DDPTGKSTMR RVPEVLDCWF ESGSMPFAQK 610 620 630 640 650 660 HYPFENKEWF DTHSPADFIV EYSGQTRGWF YTMHVLSTAL FDRPAFKKVV AHGIVLGDDG 670 680 690 700 710 720 LKMSKSKGNY PNVNEVFDRD GSDAMRWFLM SSPILRGGNL IVTEQGIREG VRQAMLPMWN 730 740 750 760 770 780 AYSFLRLYAS QPAQYDTSST NVLDRYILAK LRDTVQGVSD ALDNTDIATA CDEIRWFCDA 790 800 810 820 830 840 LTNWYVRRSR DRFWAGDAVH PEAFNTLYTV LETLCRAAAP LLPMTTEVIW RGLTGERSVH 850 860 870 880 890 900 LADFPKSENL PADAELVTAM DAVRAVSSAT SSLRKAHKLR NRLPLPKLTV ALPNASALED 910 920 930 940 950 960 FTKIIADEVN VKQVELTEDV AQVGGFEVVV NARVAGPRLG KDVQRVIKAV KSGNYTVDDA 970 980 990 1000 1010 1020 GVVTADGIEL QDGEFTRKLV AAEPEHTAEV SGQDGLVVLD TQTTPELEAE GWAADRVRGL 1030 1040 1050 1060 1070 1080 QEARKAADLQ ISDRIHLTLS VPAEKEEWAR THADSIAKEV LATDVKVVVG EQLSHDVADD 1090 CSADIVVASV AS

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI36922.1.
RefSeq	YP_250540.1. NC_007164.1.
3D structure databases
ProteinModelPortal	Q4JW85.
ModBase	Search...
Protein-protein interaction databases
STRING	306537.jk0760.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI36922; CAI36922; jk0760.
GeneID	3432445.
KEGG	cjk:jk0760.
PATRIC	21512412. VBICorJei31838_0768.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246403.
KO	K01870.
OMA	SRSRYWG.
ProtClustDB	PRK06039.
Enzyme and pathway databases
BioCyc	CJEI306537:GJ8V-789-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02003. Ile_tRNA_synth_type2.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_CORJK

Accession

Primary (citable) accession number: Q4JW85

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	August 2, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program