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Q4JW85

- SYI_CORJK

UniProt

Q4JW85 - SYI_CORJK

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei665 – 6651ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCJEI306537:GJ8V-789-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:jk0760
    OrganismiCorynebacterium jeikeium (strain K411)
    Taxonomic identifieri306537 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000545: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10921092Isoleucine--tRNA ligasePRO_0000098539Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi306537.jk0760.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4JW85.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 7111"HIGH" regionAdd
    BLAST
    Motifi662 – 6665"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4JW85-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEATPAGGA YPRVDMSNGG STAFPALEEN VLKYWEKDGT FNESVASREG     50
    NEEYVFYDGP PFANGLPHYG HLLTGYVKDI VPRYQTMQGK KVARVFGWDC 100
    HGLPAELEAE KQLGIKDRAG IEEMGMRSFN EYCATSVLRY TDEWKEYVTR 150
    QARWVDFDNG YKTMDPGFME SVMWAFKTLY DKGLIYQGFR VLPYSWAEHT 200
    PLSNQETRLD DSYKMRQDPT ITVTFPITGA TEGTASVKTL AEHPELADAA 250
    FIGWTTTPWT TPANLALAVN PEVTYAAVRI GDDAAGADGA EGMYGKTVVL 300
    ADDLRGAYAK ELGEKAEVLG TFTGAELEGM TYEPLFGYFA ERAGEKASAG 350
    EGSEGGEGNQ RKPGAFRVLC ADYVTTADGT GVVHQAPAFG EDDMFTCEAA 400
    GIDLEIPVDM DGKFTSLAPE YQGQLVFDAN KAIIKDLKAA GRVLRHQTIE 450
    HSYPHSWRSG EPLIYMALPS WFVAVTKFRD RMVELNHEQI EWMPSHIRDG 500
    QFGKWLEGAR DWNISRNRYW GAPIPVWVSD SEEYPRMDVY GSLEELERDF 550
    GRKPASLHRP DIDELTRPNP DDPTGKSTMR RVPEVLDCWF ESGSMPFAQK 600
    HYPFENKEWF DTHSPADFIV EYSGQTRGWF YTMHVLSTAL FDRPAFKKVV 650
    AHGIVLGDDG LKMSKSKGNY PNVNEVFDRD GSDAMRWFLM SSPILRGGNL 700
    IVTEQGIREG VRQAMLPMWN AYSFLRLYAS QPAQYDTSST NVLDRYILAK 750
    LRDTVQGVSD ALDNTDIATA CDEIRWFCDA LTNWYVRRSR DRFWAGDAVH 800
    PEAFNTLYTV LETLCRAAAP LLPMTTEVIW RGLTGERSVH LADFPKSENL 850
    PADAELVTAM DAVRAVSSAT SSLRKAHKLR NRLPLPKLTV ALPNASALED 900
    FTKIIADEVN VKQVELTEDV AQVGGFEVVV NARVAGPRLG KDVQRVIKAV 950
    KSGNYTVDDA GVVTADGIEL QDGEFTRKLV AAEPEHTAEV SGQDGLVVLD 1000
    TQTTPELEAE GWAADRVRGL QEARKAADLQ ISDRIHLTLS VPAEKEEWAR 1050
    THADSIAKEV LATDVKVVVG EQLSHDVADD CSADIVVASV AS 1092
    Length:1,092
    Mass (Da):121,234
    Last modified:August 2, 2005 - v1
    Checksum:iE9C5389841FBAAD6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR931997 Genomic DNA. Translation: CAI36922.1.
    RefSeqiWP_011273371.1. NC_007164.1.
    YP_250540.1. NC_007164.1.

    Genome annotation databases

    EnsemblBacteriaiCAI36922; CAI36922; jk0760.
    GeneIDi3432445.
    KEGGicjk:jk0760.
    PATRICi21512412. VBICorJei31838_0768.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR931997 Genomic DNA. Translation: CAI36922.1 .
    RefSeqi WP_011273371.1. NC_007164.1.
    YP_250540.1. NC_007164.1.

    3D structure databases

    ProteinModelPortali Q4JW85.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 306537.jk0760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI36922 ; CAI36922 ; jk0760 .
    GeneIDi 3432445.
    KEGGi cjk:jk0760.
    PATRICi 21512412. VBICorJei31838_0768.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CJEI306537:GJ8V-789-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
      Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
      J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K411.

    Entry informationi

    Entry nameiSYI_CORJK
    AccessioniPrimary (citable) accession number: Q4JW85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3