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Q4JW85

- SYI_CORJK

UniProt

Q4JW85 - SYI_CORJK

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei665 – 6651ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCJEI306537:GJ8V-789-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:jk0760
OrganismiCorynebacterium jeikeium (strain K411)
Taxonomic identifieri306537 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000545: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10921092Isoleucine--tRNA ligasePRO_0000098539Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi306537.jk0760.

Structurei

3D structure databases

ProteinModelPortaliQ4JW85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionAdd
BLAST
Motifi662 – 6665"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JW85-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEATPAGGA YPRVDMSNGG STAFPALEEN VLKYWEKDGT FNESVASREG
60 70 80 90 100
NEEYVFYDGP PFANGLPHYG HLLTGYVKDI VPRYQTMQGK KVARVFGWDC
110 120 130 140 150
HGLPAELEAE KQLGIKDRAG IEEMGMRSFN EYCATSVLRY TDEWKEYVTR
160 170 180 190 200
QARWVDFDNG YKTMDPGFME SVMWAFKTLY DKGLIYQGFR VLPYSWAEHT
210 220 230 240 250
PLSNQETRLD DSYKMRQDPT ITVTFPITGA TEGTASVKTL AEHPELADAA
260 270 280 290 300
FIGWTTTPWT TPANLALAVN PEVTYAAVRI GDDAAGADGA EGMYGKTVVL
310 320 330 340 350
ADDLRGAYAK ELGEKAEVLG TFTGAELEGM TYEPLFGYFA ERAGEKASAG
360 370 380 390 400
EGSEGGEGNQ RKPGAFRVLC ADYVTTADGT GVVHQAPAFG EDDMFTCEAA
410 420 430 440 450
GIDLEIPVDM DGKFTSLAPE YQGQLVFDAN KAIIKDLKAA GRVLRHQTIE
460 470 480 490 500
HSYPHSWRSG EPLIYMALPS WFVAVTKFRD RMVELNHEQI EWMPSHIRDG
510 520 530 540 550
QFGKWLEGAR DWNISRNRYW GAPIPVWVSD SEEYPRMDVY GSLEELERDF
560 570 580 590 600
GRKPASLHRP DIDELTRPNP DDPTGKSTMR RVPEVLDCWF ESGSMPFAQK
610 620 630 640 650
HYPFENKEWF DTHSPADFIV EYSGQTRGWF YTMHVLSTAL FDRPAFKKVV
660 670 680 690 700
AHGIVLGDDG LKMSKSKGNY PNVNEVFDRD GSDAMRWFLM SSPILRGGNL
710 720 730 740 750
IVTEQGIREG VRQAMLPMWN AYSFLRLYAS QPAQYDTSST NVLDRYILAK
760 770 780 790 800
LRDTVQGVSD ALDNTDIATA CDEIRWFCDA LTNWYVRRSR DRFWAGDAVH
810 820 830 840 850
PEAFNTLYTV LETLCRAAAP LLPMTTEVIW RGLTGERSVH LADFPKSENL
860 870 880 890 900
PADAELVTAM DAVRAVSSAT SSLRKAHKLR NRLPLPKLTV ALPNASALED
910 920 930 940 950
FTKIIADEVN VKQVELTEDV AQVGGFEVVV NARVAGPRLG KDVQRVIKAV
960 970 980 990 1000
KSGNYTVDDA GVVTADGIEL QDGEFTRKLV AAEPEHTAEV SGQDGLVVLD
1010 1020 1030 1040 1050
TQTTPELEAE GWAADRVRGL QEARKAADLQ ISDRIHLTLS VPAEKEEWAR
1060 1070 1080 1090
THADSIAKEV LATDVKVVVG EQLSHDVADD CSADIVVASV AS
Length:1,092
Mass (Da):121,234
Last modified:August 2, 2005 - v1
Checksum:iE9C5389841FBAAD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR931997 Genomic DNA. Translation: CAI36922.1.
RefSeqiYP_250540.1. NC_007164.1.

Genome annotation databases

EnsemblBacteriaiCAI36922; CAI36922; jk0760.
GeneIDi3432445.
KEGGicjk:jk0760.
PATRICi21512412. VBICorJei31838_0768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR931997 Genomic DNA. Translation: CAI36922.1 .
RefSeqi YP_250540.1. NC_007164.1.

3D structure databases

ProteinModelPortali Q4JW85.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 306537.jk0760.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI36922 ; CAI36922 ; jk0760 .
GeneIDi 3432445.
KEGGi cjk:jk0760.
PATRICi 21512412. VBICorJei31838_0768.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CJEI306537:GJ8V-789-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
    Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
    J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K411.

Entry informationi

Entry nameiSYI_CORJK
AccessioniPrimary (citable) accession number: Q4JW85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 2, 2005
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3