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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei665ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:jk0760
OrganismiCorynebacterium jeikeium (strain K411)
Taxonomic identifieri306537 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000545 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985391 – 1092Isoleucine--tRNA ligaseAdd BLAST1092

Proteomic databases

PRIDEiQ4JW85

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi306537.jk0760

Structurei

3D structure databases

ProteinModelPortaliQ4JW85
SMRiQ4JW85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 71"HIGH" regionAdd BLAST11
Motifi662 – 666"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiKMMAPFT
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q4JW85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEATPAGGA YPRVDMSNGG STAFPALEEN VLKYWEKDGT FNESVASREG
60 70 80 90 100
NEEYVFYDGP PFANGLPHYG HLLTGYVKDI VPRYQTMQGK KVARVFGWDC
110 120 130 140 150
HGLPAELEAE KQLGIKDRAG IEEMGMRSFN EYCATSVLRY TDEWKEYVTR
160 170 180 190 200
QARWVDFDNG YKTMDPGFME SVMWAFKTLY DKGLIYQGFR VLPYSWAEHT
210 220 230 240 250
PLSNQETRLD DSYKMRQDPT ITVTFPITGA TEGTASVKTL AEHPELADAA
260 270 280 290 300
FIGWTTTPWT TPANLALAVN PEVTYAAVRI GDDAAGADGA EGMYGKTVVL
310 320 330 340 350
ADDLRGAYAK ELGEKAEVLG TFTGAELEGM TYEPLFGYFA ERAGEKASAG
360 370 380 390 400
EGSEGGEGNQ RKPGAFRVLC ADYVTTADGT GVVHQAPAFG EDDMFTCEAA
410 420 430 440 450
GIDLEIPVDM DGKFTSLAPE YQGQLVFDAN KAIIKDLKAA GRVLRHQTIE
460 470 480 490 500
HSYPHSWRSG EPLIYMALPS WFVAVTKFRD RMVELNHEQI EWMPSHIRDG
510 520 530 540 550
QFGKWLEGAR DWNISRNRYW GAPIPVWVSD SEEYPRMDVY GSLEELERDF
560 570 580 590 600
GRKPASLHRP DIDELTRPNP DDPTGKSTMR RVPEVLDCWF ESGSMPFAQK
610 620 630 640 650
HYPFENKEWF DTHSPADFIV EYSGQTRGWF YTMHVLSTAL FDRPAFKKVV
660 670 680 690 700
AHGIVLGDDG LKMSKSKGNY PNVNEVFDRD GSDAMRWFLM SSPILRGGNL
710 720 730 740 750
IVTEQGIREG VRQAMLPMWN AYSFLRLYAS QPAQYDTSST NVLDRYILAK
760 770 780 790 800
LRDTVQGVSD ALDNTDIATA CDEIRWFCDA LTNWYVRRSR DRFWAGDAVH
810 820 830 840 850
PEAFNTLYTV LETLCRAAAP LLPMTTEVIW RGLTGERSVH LADFPKSENL
860 870 880 890 900
PADAELVTAM DAVRAVSSAT SSLRKAHKLR NRLPLPKLTV ALPNASALED
910 920 930 940 950
FTKIIADEVN VKQVELTEDV AQVGGFEVVV NARVAGPRLG KDVQRVIKAV
960 970 980 990 1000
KSGNYTVDDA GVVTADGIEL QDGEFTRKLV AAEPEHTAEV SGQDGLVVLD
1010 1020 1030 1040 1050
TQTTPELEAE GWAADRVRGL QEARKAADLQ ISDRIHLTLS VPAEKEEWAR
1060 1070 1080 1090
THADSIAKEV LATDVKVVVG EQLSHDVADD CSADIVVASV AS
Length:1,092
Mass (Da):121,234
Last modified:August 2, 2005 - v1
Checksum:iE9C5389841FBAAD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA Translation: CAI36922.1
RefSeqiWP_011273371.1, NC_007164.1

Genome annotation databases

EnsemblBacteriaiCAI36922; CAI36922; jk0760
GeneIDi3432445
KEGGicjk:jk0760
PATRICifig|306537.10.peg.768

Similar proteinsi

Entry informationi

Entry nameiSYI_CORJK
AccessioniPrimary (citable) accession number: Q4JW85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 2, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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