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Q4JW85

- SYI_CORJK

UniProt

Q4JW85 - SYI_CORJK

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Protein
Isoleucine--tRNA ligase
Gene
ileS, jk0760
Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei665 – 6651ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCJEI306537:GJ8V-789-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:jk0760
OrganismiCorynebacterium jeikeium (strain K411)
Taxonomic identifieri306537 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000545: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10921092Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098539Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi306537.jk0760.

Structurei

3D structure databases

ProteinModelPortaliQ4JW85.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionUniRule annotation
Add
BLAST
Motifi662 – 6665"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JW85-1 [UniParc]FASTAAdd to Basket

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MSEATPAGGA YPRVDMSNGG STAFPALEEN VLKYWEKDGT FNESVASREG     50
NEEYVFYDGP PFANGLPHYG HLLTGYVKDI VPRYQTMQGK KVARVFGWDC 100
HGLPAELEAE KQLGIKDRAG IEEMGMRSFN EYCATSVLRY TDEWKEYVTR 150
QARWVDFDNG YKTMDPGFME SVMWAFKTLY DKGLIYQGFR VLPYSWAEHT 200
PLSNQETRLD DSYKMRQDPT ITVTFPITGA TEGTASVKTL AEHPELADAA 250
FIGWTTTPWT TPANLALAVN PEVTYAAVRI GDDAAGADGA EGMYGKTVVL 300
ADDLRGAYAK ELGEKAEVLG TFTGAELEGM TYEPLFGYFA ERAGEKASAG 350
EGSEGGEGNQ RKPGAFRVLC ADYVTTADGT GVVHQAPAFG EDDMFTCEAA 400
GIDLEIPVDM DGKFTSLAPE YQGQLVFDAN KAIIKDLKAA GRVLRHQTIE 450
HSYPHSWRSG EPLIYMALPS WFVAVTKFRD RMVELNHEQI EWMPSHIRDG 500
QFGKWLEGAR DWNISRNRYW GAPIPVWVSD SEEYPRMDVY GSLEELERDF 550
GRKPASLHRP DIDELTRPNP DDPTGKSTMR RVPEVLDCWF ESGSMPFAQK 600
HYPFENKEWF DTHSPADFIV EYSGQTRGWF YTMHVLSTAL FDRPAFKKVV 650
AHGIVLGDDG LKMSKSKGNY PNVNEVFDRD GSDAMRWFLM SSPILRGGNL 700
IVTEQGIREG VRQAMLPMWN AYSFLRLYAS QPAQYDTSST NVLDRYILAK 750
LRDTVQGVSD ALDNTDIATA CDEIRWFCDA LTNWYVRRSR DRFWAGDAVH 800
PEAFNTLYTV LETLCRAAAP LLPMTTEVIW RGLTGERSVH LADFPKSENL 850
PADAELVTAM DAVRAVSSAT SSLRKAHKLR NRLPLPKLTV ALPNASALED 900
FTKIIADEVN VKQVELTEDV AQVGGFEVVV NARVAGPRLG KDVQRVIKAV 950
KSGNYTVDDA GVVTADGIEL QDGEFTRKLV AAEPEHTAEV SGQDGLVVLD 1000
TQTTPELEAE GWAADRVRGL QEARKAADLQ ISDRIHLTLS VPAEKEEWAR 1050
THADSIAKEV LATDVKVVVG EQLSHDVADD CSADIVVASV AS 1092
Length:1,092
Mass (Da):121,234
Last modified:August 2, 2005 - v1
Checksum:iE9C5389841FBAAD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR931997 Genomic DNA. Translation: CAI36922.1.
RefSeqiWP_011273371.1. NC_007164.1.
YP_250540.1. NC_007164.1.

Genome annotation databases

EnsemblBacteriaiCAI36922; CAI36922; jk0760.
GeneIDi3432445.
KEGGicjk:jk0760.
PATRICi21512412. VBICorJei31838_0768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR931997 Genomic DNA. Translation: CAI36922.1 .
RefSeqi WP_011273371.1. NC_007164.1.
YP_250540.1. NC_007164.1.

3D structure databases

ProteinModelPortali Q4JW85.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 306537.jk0760.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI36922 ; CAI36922 ; jk0760 .
GeneIDi 3432445.
KEGGi cjk:jk0760.
PATRICi 21512412. VBICorJei31838_0768.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CJEI306537:GJ8V-789-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
    Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
    J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K411.

Entry informationi

Entry nameiSYI_CORJK
AccessioniPrimary (citable) accession number: Q4JW85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 2, 2005
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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