Reviewed, UniProtKB/Swiss-Prot Q4JW03 (ARGJ_CORJK)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	jk0842

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity. HAMAP MF_01106
Subcellular location	Cytoplasm Probable HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000227220
	Chain	200 – 405	206	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000227221
Sites
	Site	199 – 200	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JW03-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 41D43BABA17733E7	FASTA	405	41,272
	10 20 30 40 50 60 MTSADKNNPD TSTAQGSSAD LGVTVPKGFS AAAVTAGIKP SGKSDMALIR NDGPDDIAAA 70 80 90 100 110 120 MFTRNQVTAA PVRYTKANND GTFRAVVVNS GNANACNGAQ GDKDARATAE KAAELLGCQP 130 140 150 160 170 180 GDVAVCSTGL IGDVLPMDKV LSGVDELAGK LDQSITAGND AARAIMTTDL VAKEAVYHGD 190 200 210 220 230 240 GWSIGAMGKG VGMMAPSLAT MLVFITTDAH LPSADMAHEA LAGATPTTFD CIDIDGATST 250 260 270 280 290 300 NDTVLLMASG ASGITPDAEE FNAAIHQVCL DIAMQLQADA EGVTKRVSIT VGGAETDADA 310 320 330 340 350 360 QTAARVIGRD NLFKCAMFGS DPNWGRVLAA VGMAPVKMNP EAISVSFNGH PVCINSTGAP 370 380 390 400 GARTVDLSGA DIAVEVDLGV GEGRATVWTT DLSYSYVEIN SEYST

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI37004.1.
RefSeq	YP_250622.1.
3D structure databases
SMR	Q4JW03. Positions 20-199, 25-402, 200-403.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JW03.
Genome annotation databases
GeneID	3432218.
GenomeReviews	Gene locus jk0842 in contig CR931997_GR.
KEGG	cjk:jk0842.
NMPDR	fig|306537.3.peg.2034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1364.
HOGENOM	HBG284202.
OMA	VHENSAY.
Enzyme and pathway databases
BioCyc	CJEI306537:JK0842-MONOMER.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_CORJK

Accession

Primary (citable) accession number: Q4JW03

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)