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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase 2

Gene

mshC2

Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44ZincUniRule annotation1
Binding sitei59Cysteinyl adenylateUniRule annotation1
Binding sitei228Cysteinyl adenylateUniRule annotation1
Metal bindingi232ZincUniRule annotation1
Metal bindingi257ZincUniRule annotation1
Binding sitei284Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase 2UniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligase 2UniRule annotation
Alternative name(s):
Mycothiol ligase 2UniRule annotation
Short name:
MSH ligase 2UniRule annotation
Gene namesi
Name:mshC2UniRule annotation
Ordered Locus Names:jk0956
OrganismiCorynebacterium jeikeium (strain K411)
Taxonomic identifieri306537 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000545 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004004401 – 415L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase 2Add BLAST415

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi306537.jk0956.

Structurei

3D structure databases

ProteinModelPortaliQ4JVN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 47Cysteinyl adenylate bindingUniRule annotation4
Regioni82 – 84Cysteinyl adenylate bindingUniRule annotation3
Regioni250 – 252Cysteinyl adenylate bindingUniRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 56"HIGH" regionUniRule annotationAdd BLAST11
Motifi188 – 193"ERGGDP" regionUniRule annotation6
Motifi290 – 294"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHIECATI.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4JVN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSWPEPEVP QLPGEAPQLR LYDTADDVVK PVEIPAGEVG MYVCGITPYD
60 70 80 90 100
STHLGHAATY LTFDLIHRYL RAAGHGVHYV QNITDVDDPL FERAERDGVD
110 120 130 140 150
WQDLGTSQID LFRSDMEDLA VIPPRDYIGA MESIDEVIEM VAKLLEVGAA
160 170 180 190 200
YQLEGDEYPD IYADYTFLPQ FGYESRYDEQ QMAEFFAERG GDPERPGKRH
210 220 230 240 250
PMDALLWRAH REGEPKWDSP FGPGRPGWHI ECSAIAVNRL GAPFAIQGGG
260 270 280 290 300
SDLRFPHHEF SATHAEAAHG VDRMAHHYVH TGMIGLDGTK MSKSLGNLVF
310 320 330 340 350
VSRLTAAGHE PAAIRLGVYA GHYRQDRDWT NDVLAHAEHR LASWRAALAT
360 370 380 390 400
GSTTLDEARE LVATVRGHLA NDLDTAAALE ALDDWAADTK TGAAGDEAAA
410
SEIKTAIDAL LGVRL
Length:415
Mass (Da):45,686
Last modified:August 2, 2005 - v1
Checksum:iBA7F40DC60DDB887
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA. Translation: CAI37120.1.
RefSeqiWP_011273536.1. NC_007164.1.

Genome annotation databases

EnsemblBacteriaiCAI37120; CAI37120; jk0956.
GeneIDi3433753.
KEGGicjk:jk0956.
PATRICi21512820. VBICorJei31838_0967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA. Translation: CAI37120.1.
RefSeqiWP_011273536.1. NC_007164.1.

3D structure databases

ProteinModelPortaliQ4JVN7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi306537.jk0956.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI37120; CAI37120; jk0956.
GeneIDi3433753.
KEGGicjk:jk0956.
PATRICi21512820. VBICorJei31838_0967.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHIECATI.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC2_CORJK
AccessioniPrimary (citable) accession number: Q4JVN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.