Q4JVM4 (ACON_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aconitate hydratase
EC=4.2.1.3

Gene names

Name:	acn
Ordered Locus Names:	jk0969

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	936 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Induction	Repressed by AcnR By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro aconitate hydratase activity Inferred from electronic annotation. Source: EC citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 936	936	Aconitate hydratase		PRO_0000076660
Sites
	Metal binding	472	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	538	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	541	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JVM4 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 55B605AD2F117308	FASTA	936	101,295
	10 20 30 40 50 60 MAESINSFDS KSTLQVGEKS YDYFALDAVP GMEKLPYSLK VLGENLLRNE DGKNITREHI 70 80 90 100 110 120 EAIANWDPSA EPNFEIQFTP ARVIMQDFTG VACIVDLATI RDAVVALGGD ADDVNPLNPA 130 140 150 160 170 180 EMVIDHSVII EAFGDSDALE KNVEIEYQRN DERYKFLRWG TGAFENFRVV PPGTGIVHQV 190 200 210 220 230 240 NIEYLARSVF DNNGLAYPDT CVGTDSHTTM ENGLGILGWG VGGIEAEAAM LGQPISMLIP 250 260 270 280 290 300 RVVGFKLTGE IPTGVTATDV VLTITDMLRQ HGVVGKFVEF YGKGVGELPL ANRATIGNMS 310 320 330 340 350 360 PEFGSTAAMF PIDEETVKYL ELTGRDQETL ERVEAYAKAQ GMWLDPEKEV EYSEYLELDL 370 380 390 400 410 420 STVVPSIAGP KRPQDRIELN DSKAQFRKDL HNYVEADASA VTPDFDAEGP ATENTSAQTA 430 440 450 460 470 480 GTPASAADAK GNIPSAAAGA EGRPSNPVTV NYNGEDIELD HGMVAIASIT SCTNTSNPSV 490 500 510 520 530 540 MVGAGLLARN AAAKGLKSAP WVKTSMAPGS QVVNGYYEKA GLWKDLEAMG FYLVGYGCTT 550 560 570 580 590 600 CIGNSGPLPE EISAGINEGD LAATAVLSGN RNFEGRINPD VKMNYLASPI LVIAYAIAGT 610 620 630 640 650 660 MDFDFETQPL GQDQDGNDVF LKDIWPSTED IEEVIASSIT KDLYAEDYAN VFEGDERWRS 670 680 690 700 710 720 LDVPSGKTFD WDPKSTYIRK APYFDGMSKE PEAVNDVKGA RVLALLGDSV TTDHISPAST 730 740 750 760 770 780 IKPGTPAAQY LDANGVERKD YNSLGARRGN HEVMVRGTFA NIRLQNQLLD GVSGGYTRDF 790 800 810 820 830 840 TQEGGPQSFI YDAAMNYQKE NTPLVVLGGK EYGTGSSRDW AAKGTLLLGV KAVIAESFER 850 860 870 880 890 900 IHRSNLIGMG VVPLQFPEGE SWKSLGIEGT ETFDIEGIEE LNNGSTPKTV KVTATKENGE 910 920 930 KIEFDAVTRI DTPGEADYYR NGGILQFVLR NMMSGK

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI37133.1.
RefSeq	YP_250751.1. NC_007164.1.
3D structure databases
ProteinModelPortal	Q4JVM4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JVM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3432029.
GenomeReviews	Gene locus jk0969 in contig CR931997_GR.
KEGG	cjk:jk0969.
NMPDR	fig|306537.3.peg.429.
PATRIC	21512848. VBICorJei31838_0980.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1048.
HOGENOM	HBG289738.
OMA	YSKAQGM.
ProtClustDB	PRK09277.
Enzyme and pathway databases
BioCyc	CJEI306537:JK0969-MONOMER.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 3 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
KO	K01681.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 2 hits. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR01341. Aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_CORJK

Accession

Primary (citable) accession number: Q4JVM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program