Q4JV64 (DAPA_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	jk1129

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050181
Regions
	Region	60 – 61	2	Pyruvate binding By similarity
Sites
	Active site	173	1	Schiff-base intermediate with substrate By similarity
	Binding site	118	1	Pyruvate By similarity
	Site	145	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JV64 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 16397BE38754458D	FASTA	300	31,293
	10 20 30 40 50 60 MSTGNAATRG SAHFGTISLA MVTPFKKDGS IDLDAGVALA GHFVDEGCDS LVLAGTTGES 70 80 90 100 110 120 PTTGATEKLD LLRAVRSELG DSVKLIAGSG SYDTAVTVEM SRASQEAGAD SLLVVTPYYS 130 140 150 160 170 180 RPSQEGIYQH FTTVADAVDI PVCVYDIPSR SVVPVEPETL HRLADHPQIA AVKDAKGDLA 190 200 210 220 230 240 AGMELIENTD LAWYSGDDPL NLPWLAAGAT GFISVIGHVA TRQLKQLRDA FDAGDIATAR 250 260 270 280 290 300 SIAVQLQPLQ KAQARLGGVT FAKAALKLKG KDVGAPRLPI IEPTAAEMDQ LAQDLQAAGV

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI37293.1.
RefSeq	YP_250911.1. NC_007164.1.
3D structure databases
HSSP	HSSP built from PDB template 1XXX based on UniProtKB P63945.
ProteinModelPortal	Q4JV64.
SMR	Q4JV64. Positions 11-300.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JV64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3433759.
GenomeReviews	Gene locus jk1129 in contig CR931997_GR.
KEGG	cjk:jk1129.
NMPDR	fig|306537.3.peg.1168.
PATRIC	21513186. VBICorJei31838_1142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	HGCISVV.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	CJEI306537:JK1129-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CORJK

Accession

Primary (citable) accession number: Q4JV64

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program