Q4JV46 (SYP_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	jk1147

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Proline--tRNA ligase HAMAP MF_01569		PRO_0000248679

Sequences

	Sequence		Length	Mass (Da)
	Q4JV46 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 6B2EC061E4C3D75C	FASTA	596	65,338
	10 20 30 40 50 60 MITRMSQLFL RTLRDDPADA EVPSHKLLVR AGYIRRVAPG VYSWLPLGLR VLRNIEAVVR 70 80 90 100 110 120 EEMNAIGAQE ISFPALLPRE PYDTTGRWVE YGDALFRLKD RKNADYLLGP THEEMFTSVV 130 140 150 160 170 180 KSEYNSYKDF PVILYQVQTK YRDEERPRAG ILRGREFIMK DSYSFNMDDE GLEESYQLHR 190 200 210 220 230 240 KAYQNIFDRL GIKYAICSAT SGAMGGSASE EFLAVAANGE DTFVRSTESD YAANVEAVVT 250 260 270 280 290 300 QVPPAQPIEG QPEAKEYETP DSETIEALVE WANSEGITID GRAVEAADTL KCIVIKVNDP 310 320 330 340 350 360 TLDEEGKPRG ESLAGVLVPG NREVDMKRLE ASMEPAQVEL ADEADFKKNP FLVKGYVGPK 370 380 390 400 410 420 GLAANGVRVL ADPRVVDGTS WITGADAKNR HVVGLVAGRD FTPDGFVEAA EVLEGDPSPD 430 440 450 460 470 480 GKGTLALARG IEIGHIFQLG RKYTEAFDVK ILDESGKRSV PTMGSYGVGV SRLVGVIAEQ 490 500 510 520 530 540 MLDDSGLRWP ASVAPFDVHV VIANKDAAAG EAAVELVETL SNDGLEVLFD DRPKVSPGVK 550 560 570 580 590 FKDSELLGMP LVVVVGRGFA DGKVELRNRL TGETYEVDYT DALPEAKRAL RGEARP

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI37311.1.
RefSeq	YP_250929.1. NC_007164.1.
3D structure databases
ProteinModelPortal	Q4JV46.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JV46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3432984.
GenomeReviews	Gene locus jk1147 in contig CR931997_GR.
KEGG	cjk:jk1147.
NMPDR	fig|306537.3.peg.1291.
PATRIC	21513222. VBICorJei31838_1160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	CJEI306537:JK1147-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_CORJK

Accession

Primary (citable) accession number: Q4JV46

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program