Q4JV03 (TRMD_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	jk1190

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060366
Regions
	Region	173 – 178	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	149	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JV03 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: A5E978F4EE32FA3C	FASTA	282	30,774
	10 20 30 40 50 60 MRLDVVTIFP EYLEPLRHAL LGKAIEQGIL SVGVHNLRAW TNDVHQSVDS TPCGGGPGMV 70 80 90 100 110 120 MKPEVWGPAL DDVAAGTGPA ATVSDLESSA EHKRNLRPAT TNGDAEPLGE KAGGVTKPLL 130 140 150 160 170 180 IVPTPAGKPF TQDMAERWST EEHVVFACGR YEGIDQRVVE DAHNRYRVEE VSIGDYVLIG 190 200 210 220 230 240 GEVAVLVMAE AMVRLIPGVL GNQASHQEDS FQDGLLEGPS YTRPRQWRGL DVPDVLFSGN 250 260 270 280 HAKIAQWRRE QSLARTKKIR PDLLDSVELS KADRAYLDGL EL

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI37354.1.
RefSeq	YP_250972.1. NC_007164.1.
3D structure databases
ProteinModelPortal	Q4JV03.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JV03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3433461.
GenomeReviews	Gene locus jk1190 in contig CR931997_GR.
KEGG	cjk:jk1190.
NMPDR	fig|306537.3.peg.1419.
PATRIC	21513310. VBICorJei31838_1204.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	CJEI306537:JK1190-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 2 hits. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_CORJK

Accession

Primary (citable) accession number: Q4JV03

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program