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Q4JUQ7 (SYE_CORJK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:jk1281
OrganismCorynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]
Taxonomic identifier306537 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000237355

Regions

Motif13 – 2311"HIGH" region HAMAP-Rule MF_00022
Motif258 – 2625"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2611ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4JUQ7 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 25467C78413C01D8

FASTA50056,360
        10         20         30         40         50         60 
MSEVSEVRVR FCPSPTGTPH VGMVRTALFN WAYARHTGGK LVFRIEDTDA KRDSEESYQA 

        70         80         90        100        110        120 
IIDSLKWLNL GWDEGIEVGG PHEPYRQSQR MDIYADVLEK LKESGDVYPA YSTNEEVQAR 

       130        140        150        160        170        180 
HKAAGRDPQL GYDNYDRDLT EEQIAQFKAE GREPVWRLRM PDNRIYEWHD LVRGDMSVDG 

       190        200        210        220        230        240 
KTVPDFVVAR SNGQPLYTLV NPVDDALMEI THVLRGEDLL PSTPRQIALY EALVRVGVAK 

       250        260        270        280        290        300 
QVPTFGHLPF VMGEGNKKLS KRDPESNLFN HRDNGIIPEG MLNYLSLLGW SLSADEDIFT 

       310        320        330        340        350        360 
MQQLIDNFDV ADVKPNPARF DQKKLEAINA DHIRMLELGD FTERLRAYLE EYKGFPADYP 

       370        380        390        400        410        420 
ADKFAFAAEL VQTRIKTLSD ADGLLRFLIT SDADLSLDEK AARKNLKEDA VEVLEVAIAQ 

       430        440        450        460        470        480 
LEAIADGEFK TDVIEKALQS KLIEEMELKP RKAYGALRVA ISGAAVSPPL FESMELLGKE 

       490        500 
STLARLRAAR EQTPFAPAQQ 

« Hide

References

[1]"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K411.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR931997 Genomic DNA. Translation: CAI37450.1.
RefSeqYP_251068.1. NC_007164.1.

3D structure databases

ProteinModelPortalQ4JUQ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING306537.jk1281.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI37450; CAI37450; jk1281.
GeneID3433773.
KEGGcjk:jk1281.
PATRIC21513498. VBICorJei31838_1297.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAPEGMLNY.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycCJEI306537:GJ8V-1326-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_CORJK
AccessionPrimary (citable) accession number: Q4JUQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: August 2, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries