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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49NADPUniRule annotation1
Binding sitei52NADPUniRule annotation1
Binding sitei54NADPUniRule annotation1
Active sitei109UniRule annotation1
Binding sitei135NADP; via amide nitrogenUniRule annotation1
Metal bindingi192Magnesium 1UniRule annotation1
Metal bindingi192Magnesium 2UniRule annotation1
Metal bindingi196Magnesium 1UniRule annotation1
Metal bindingi228Magnesium 2UniRule annotation1
Metal bindingi232Magnesium 2UniRule annotation1
Binding sitei253SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 29NADPUniRule annotation4
Nucleotide bindingi84 – 87NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Ketol-acid reductoisomerase type 1UniRule annotation
Ketol-acid reductoisomerase type IUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:jk1298
OrganismiCorynebacterium jeikeium (strain K411)
Taxonomic identifieri306537 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000545 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002261721 – 338Ketol-acid reductoisomerase (NADP(+))Add BLAST338

Interactioni

Protein-protein interaction databases

STRINGi306537.jk1298

Structurei

3D structure databases

ProteinModelPortaliQ4JUN9
SMRiQ4JUN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 183KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST181
Domaini184 – 329KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6M Bacteria
COG0059 LUCA
HOGENOMiHOG000016230
KOiK00053
OMAiRAMFSWL
OrthoDBiPOG091H063Y

Family and domain databases

HAMAPiMF_00435 IlvC, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR000506 AcH_isomrdctse_C
IPR013116 IlvN
IPR013023 KARI
IPR014359 KARI_prok
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 1 hit
PF07991 IlvN, 1 hit
PIRSFiPIRSF000116 IlvC_gammaproteo, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 1 hit
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q4JUN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDVFYDDD ADLSIIQGRK VAIIGYGSQG HAHAQNLRES GVEVVIGLRE
60 70 80 90 100
GSKSRAKAEE AGFTVKTNAE AAEWADVIML LAPDTSQAKI FAEDIEPNLN
110 120 130 140 150
DGDALFFGHG LNIHFKLIEP ADNIIIGMVA PKGPGHLVRR QFVDGKGVPC
160 170 180 190 200
LIAVEQDPKN NGRDLALSYA AAIGGARAGV IPTTFEAETV TDLFGEQAVL
210 220 230 240 250
CGGTEELIKT GFEVLVEAGY EPEMAYFECL HEMKLIVDLM FEGGIANMNY
260 270 280 290 300
SVSDTAEFGG YLSGPRVIDA DTKKRMKDIL TDIQDGTFTK RLVANVEGGN
310 320 330
KELEELRASY NDHQIEKTGA KLRDLMSWVK VDARAETA
Length:338
Mass (Da):36,640
Last modified:August 2, 2005 - v1
Checksum:iC3615775C35B4099
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA Translation: CAI37468.1
RefSeqiWP_011273793.1, NC_007164.1

Genome annotation databases

EnsemblBacteriaiCAI37468; CAI37468; jk1298
GeneIDi3432448
KEGGicjk:jk1298
PATRICifig|306537.10.peg.1317

Similar proteinsi

Entry informationi

Entry nameiILVC_CORJK
AccessioniPrimary (citable) accession number: Q4JUN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 2, 2005
Last modified: February 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome