Q4JUN3 (ILVD_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	jk1303

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225385
Sites
	Metal binding	124	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	199	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4JUN3 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 2E0999F950337157	FASTA	615	65,041
	10 20 30 40 50 60 MNAIPLRSAV TTQGRNAAGA RALWRATGMT DQDFEKPIIA IANSYTQFVP GHVHLKDVGD 70 80 90 100 110 120 IVADAIREAG GVPREFNTIA VDDGIAMGHA GMLYSLPSRE VISDAVEYMV NGHAADALVC 130 140 150 160 170 180 ISNCDKITPG MLNAAMRLNI PVVFVSGGPM EAGKAVAVDG VVQAPTDLIT AISASASDKV 190 200 210 220 230 240 DAQALLEVES AACPTCGSCS GMFTANSMNC LTEALGLSLP GNGSTLATHA KRRDLFTKAG 250 260 270 280 290 300 TTIVELCRRY YGEGDASVLP RNIATREAFE NAMALDMAMG GSSNTVLHIL AAAQEGEIDF 310 320 330 340 350 360 DLHSIDQLSR EVPCLSKVSP NSDYHMEDVH RGGGIPAILG ELYRAGKLNE GVHSVHSATL 370 380 390 400 410 420 KEWLLSWDIR SGAATEEAIE LFHAAPGGVR TTAPFSTENR WSSLDTDAEG GVIRDVEHAY 430 440 450 460 470 480 TADGGLVVLR GNLAEDGAVI KAAGVDPSIW HFEGKALVVE SQEEAVRVIL DKKVKPGHVV 490 500 510 520 530 540 VVRYEGPAGG PGMQEMLHPT AFLKGAGLGK ECALITDGRF SGGTSGLSVG HISPEAAHGG 550 560 570 580 590 600 LIGLVRDGDP ITIDVHERLL QLNISDEEIE RRRAEMEASD KPWTPTTRQR KVTKALRAYA 610 AMATSADRGA VREVK

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI37474.1.
RefSeq	YP_251092.1. NC_007164.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4JUN3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3432449.
GenomeReviews	Gene locus jk1303 in contig CR931997_GR.
KEGG	cjk:jk1303.
NMPDR	fig|306537.3.peg.2057.
PATRIC	21513548. VBICorJei31838_1322.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	CJEI306537:JK1303-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CORJK

Accession

Primary (citable) accession number: Q4JUN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program