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Q4JUL8 (DNLJ_CORJK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:jk1317
OrganismCorynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]
Taxonomic identifier306537 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length695 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 695695DNA ligase HAMAP MF_01588
PRO_0000313208

Regions

Domain599 – 68890BRCT
Nucleotide binding30 – 345NAD By similarity
Nucleotide binding81 – 822NAD By similarity

Sites

Active site1081N6-AMP-lysine intermediate By similarity
Metal binding4031Zinc By similarity
Metal binding4061Zinc By similarity
Metal binding4221Zinc By similarity
Metal binding4281Zinc By similarity
Binding site1061NAD By similarity
Binding site1291NAD By similarity
Binding site1691NAD By similarity
Binding site2851NAD By similarity
Binding site3091NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4JUL8 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 55694077ADB29A1C

FASTA69575,611
        10         20         30         40         50         60 
MSTQSRWQEL ADQVRHHRQL YYYGEPEISD ADFDALLQEL KDLEQAHPEV VTGASPTEEV 

        70         80         90        100        110        120 
APAPPTSSPF RNVDHREQML SLDNVFDTEQ LAGWLDRTPA KTYLTELKID GASINLLYID 

       130        140        150        160        170        180 
GQLELALTRG DGTTGEDITH NARTLDDIPD TLHGTDEFPV PELVEIRGEV FIDVEDFAAM 

       190        200        210        220        230        240 
NAQRQAEGLK MFANPRNAAA GAMRQKNSAD TAKRPLKLIC HGIGAREGFS PASQHDAYRA 

       250        260        270        280        290        300 
IAAWGLPVSP YTKQVHSAKE VQQQVEYWGN HRHDAAHEMD GLVVKVDSLE EQLELGHTSR 

       310        320        330        340        350        360 
APRWAIAYKY PPEEAMTTLH NIRVGIGRTG RATPYAVMAP KYVAGSTVSM ATLHNPTEAH 

       370        380        390        400        410        420 
RKGVLLGDTI TIRKAGEVIP EVLGPVEDKR TGAERPFLFS TFCPECGTRL APSKVGDADW 

       430        440        450        460        470        480 
RCPNTQYCPG QLHTRLTYLA SRKAFDIDAL GEKGAYDLIH SGVLADESEL FDLRAEDLEK 

       490        500        510        520        530        540 
TSSYATKAGK LNKSGETLLE KLQSAKEADL WRVLVALSIR HVGPTAAKAL AKHFESVEDI 

       550        560        570        580        590        600 
ASASAEEMAG IDGVAETIAE SISDWFTVDW HRRIVDRWAA AGVRMRREAG NVPGAADGVD 

       610        620        630        640        650        660 
SALLEGLTIV VTGSLEDFDR TAAKEAIEAR GGKAAGSVSK KTDFLVAGEK AGSKLKKAED 

       670        680        690 
LGVPVLDEAA FKELLENGAP SSGDDASTSA DSVDD

« Hide

References

[1]"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
J. Bacteriol. 187:4671-4682(2005) [PubMed: 15968079] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K411.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR931997 Genomic DNA. Translation: CAI37489.1.
RefSeqYP_251107.1. NC_007164.1.

3D structure databases

HSSPHSSP built from PDB template 1L7B based on UniProtKB P26996.
ProteinModelPortalQ4JUL8.
SMRQ4JUL8. Positions 3-313.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ4JUL8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3433569.
GenomeReviewsGene locus jk1317 in contig CR931997_GR.
KEGGcjk:jk1317.
NMPDRfig|306537.3.peg.2071.
PATRIC21513578. VBICorJei31838_1337.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0272.
HOGENOMHBG620317.
OMAENVRTIR.
ProtClustDBPRK07956.

Enzyme and pathway databases

BioCycCJEI306537:JK1317-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_CORJK
AccessionPrimary (citable) accession number: Q4JUL8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 2, 2005
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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