Q4JU18 (SYM_CORJK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	jk1516

Organism

Corynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]

Taxonomic identifier

306537 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium jeikeium

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_0000331808
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	350 – 354	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	144	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	157	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Binding site	353	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JU18 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: E01E6207EFE8ED13	FASTA	612	68,132
	10 20 30 40 50 60 MSKRVLTSVA WPYANGPRHI GHVAGFGVPS DVFARYQRMA GNEVLMVSGT DEHGTPLLVQ 70 80 90 100 110 120 AEKEGTTVQE LADRYNRIIV EDLAGLGLSY DLFTRTTTRN HYAVVQELFT GLYNNGYMVT 130 140 150 160 170 180 QTTRGAISPS TGRTLPDRYI EGTCPICGAK DARGDQCDNC GNQLDPEDLI DPRSKINGET 190 200 210 220 230 240 PEFIDTEHFM LDLPALAEAL TTWLKGRTDW RPNVLKFSLN LLEDIRPRAM SRDIDWGVPI 250 260 270 280 290 300 PVEGWQDNNA KKLYVWFDAV VGYLSASIEW AWRIGDPDAW RKWWNDPEAL SYYFMGKDNI 310 320 330 340 350 360 TFHSQIWPAE LLGYGGQGSK GGEAGDLADP ALNLPTEVVS SEFLTMSGSK FSSSKGVVIY 370 380 390 400 410 420 VRDFLKEFGP DALRYFIATA GPETTDTDFT WDEFVRRINS ELANEWGNLV NRTVSMAYKN 430 440 450 460 470 480 FGEVPTPGPL TAEDEALLAE SAKAFDVVGD NINLSRFKAG ITEAMRIAGR ANQYIAAMEP 490 500 510 520 530 540 WKLAKDENQR ERLATVLYTA LQVVSDVNTL LTPYLPFSAQ KIFETLGGEG IWAAQPEVVE 550 560 570 580 590 600 VTDESPREPV GVGLPEEGRT YPVIMGDYTK QKVTWARQEI QPGTQLSKPK PLFPKLDPEL 610 AETGPEWAPV QK

References

[1]

"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora." Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A. J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR931997 Genomic DNA. Translation: CAI37689.1.
RefSeq	YP_251307.1. NC_007164.1.
3D structure databases
ProteinModelPortal	Q4JU18.
ModBase	Search...
Protein-protein interaction databases
STRING	306537.jk1516.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI37689; CAI37689; jk1516.
GeneID	3433307.
KEGG	cjk:jk1516.
PATRIC	21513982. VBICorJei31838_1536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200402.
KO	K01874.
OMA	SYYFMGK.
ProtClustDB	PRK12268.
Enzyme and pathway databases
BioCyc	CJEI306537:GJ8V-1564-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_CORJK

Accession

Primary (citable) accession number: Q4JU18

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 2, 2005
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program