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Q4JU18 (SYM_CORJK) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase

EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:jk1516
OrganismCorynebacterium jeikeium (strain K411) [Complete proteome] [HAMAP]
Taxonomic identifier306537 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length612 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00098

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00098

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 612612Methionine--tRNA ligase HAMAP-Rule MF_00098
PRO_0000331808

Regions

Motif12 – 2211"HIGH" region HAMAP-Rule MF_00098
Motif350 – 3545"KMSKS" region HAMAP-Rule MF_00098

Sites

Metal binding1441Zinc By similarity
Metal binding1471Zinc By similarity
Metal binding1571Zinc By similarity
Metal binding1601Zinc By similarity
Binding site3531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4JU18 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: E01E6207EFE8ED13

FASTA61268,132
        10         20         30         40         50         60 
MSKRVLTSVA WPYANGPRHI GHVAGFGVPS DVFARYQRMA GNEVLMVSGT DEHGTPLLVQ 

        70         80         90        100        110        120 
AEKEGTTVQE LADRYNRIIV EDLAGLGLSY DLFTRTTTRN HYAVVQELFT GLYNNGYMVT 

       130        140        150        160        170        180 
QTTRGAISPS TGRTLPDRYI EGTCPICGAK DARGDQCDNC GNQLDPEDLI DPRSKINGET 

       190        200        210        220        230        240 
PEFIDTEHFM LDLPALAEAL TTWLKGRTDW RPNVLKFSLN LLEDIRPRAM SRDIDWGVPI 

       250        260        270        280        290        300 
PVEGWQDNNA KKLYVWFDAV VGYLSASIEW AWRIGDPDAW RKWWNDPEAL SYYFMGKDNI 

       310        320        330        340        350        360 
TFHSQIWPAE LLGYGGQGSK GGEAGDLADP ALNLPTEVVS SEFLTMSGSK FSSSKGVVIY 

       370        380        390        400        410        420 
VRDFLKEFGP DALRYFIATA GPETTDTDFT WDEFVRRINS ELANEWGNLV NRTVSMAYKN 

       430        440        450        460        470        480 
FGEVPTPGPL TAEDEALLAE SAKAFDVVGD NINLSRFKAG ITEAMRIAGR ANQYIAAMEP 

       490        500        510        520        530        540 
WKLAKDENQR ERLATVLYTA LQVVSDVNTL LTPYLPFSAQ KIFETLGGEG IWAAQPEVVE 

       550        560        570        580        590        600 
VTDESPREPV GVGLPEEGRT YPVIMGDYTK QKVTWARQEI QPGTQLSKPK PLFPKLDPEL 

       610 
AETGPEWAPV QK 

« Hide

References

[1]"Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora."
Tauch A., Kaiser O., Hain T., Goesmann A., Weisshaar B., Albersmeier A., Bekel T., Bischoff N., Brune I., Chakraborty T., Kalinowski J., Meyer F., Rupp O., Schneiker S., Viehoever P., Puehler A.
J. Bacteriol. 187:4671-4682(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K411.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR931997 Genomic DNA. Translation: CAI37689.1.
RefSeqYP_251307.1. NC_007164.1.

3D structure databases

ProteinModelPortalQ4JU18.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING306537.jk1516.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI37689; CAI37689; jk1516.
GeneID3433307.
KEGGcjk:jk1516.
PATRIC21513982. VBICorJei31838_1536.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHOG000200402.
KOK01874.
OMAFKTETEI.
OrthoDBEOG6CVV9B.

Enzyme and pathway databases

BioCycCJEI306537:GJ8V-1564-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 2 hits.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00098. Met_tRNA_synth_type1.
InterProIPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF57770. SSF57770. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYM_CORJK
AccessionPrimary (citable) accession number: Q4JU18
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 2, 2005
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries