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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Corynebacterium jeikeium (strain K411)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144ZincUniRule annotation1
Metal bindingi147ZincUniRule annotation1
Metal bindingi157ZincUniRule annotation1
Metal bindingi160ZincUniRule annotation1
Binding sitei353ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:jk1516
OrganismiCorynebacterium jeikeium (strain K411)
Taxonomic identifieri306537 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000545 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003318081 – 612Methionine--tRNA ligaseAdd BLAST612

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi306537.jk1516.

Structurei

3D structure databases

ProteinModelPortaliQ4JU18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionAdd BLAST11
Motifi350 – 354"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0143. LUCA.
HOGENOMiHOG000200402.
KOiK01874.
OMAiFWPAMLD.
OrthoDBiPOG091H0269.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 2 hits.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4JU18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRVLTSVA WPYANGPRHI GHVAGFGVPS DVFARYQRMA GNEVLMVSGT
60 70 80 90 100
DEHGTPLLVQ AEKEGTTVQE LADRYNRIIV EDLAGLGLSY DLFTRTTTRN
110 120 130 140 150
HYAVVQELFT GLYNNGYMVT QTTRGAISPS TGRTLPDRYI EGTCPICGAK
160 170 180 190 200
DARGDQCDNC GNQLDPEDLI DPRSKINGET PEFIDTEHFM LDLPALAEAL
210 220 230 240 250
TTWLKGRTDW RPNVLKFSLN LLEDIRPRAM SRDIDWGVPI PVEGWQDNNA
260 270 280 290 300
KKLYVWFDAV VGYLSASIEW AWRIGDPDAW RKWWNDPEAL SYYFMGKDNI
310 320 330 340 350
TFHSQIWPAE LLGYGGQGSK GGEAGDLADP ALNLPTEVVS SEFLTMSGSK
360 370 380 390 400
FSSSKGVVIY VRDFLKEFGP DALRYFIATA GPETTDTDFT WDEFVRRINS
410 420 430 440 450
ELANEWGNLV NRTVSMAYKN FGEVPTPGPL TAEDEALLAE SAKAFDVVGD
460 470 480 490 500
NINLSRFKAG ITEAMRIAGR ANQYIAAMEP WKLAKDENQR ERLATVLYTA
510 520 530 540 550
LQVVSDVNTL LTPYLPFSAQ KIFETLGGEG IWAAQPEVVE VTDESPREPV
560 570 580 590 600
GVGLPEEGRT YPVIMGDYTK QKVTWARQEI QPGTQLSKPK PLFPKLDPEL
610
AETGPEWAPV QK
Length:612
Mass (Da):68,132
Last modified:August 2, 2005 - v1
Checksum:iE01E6207EFE8ED13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA. Translation: CAI37689.1.
RefSeqiWP_011273939.1. NC_007164.1.

Genome annotation databases

EnsemblBacteriaiCAI37689; CAI37689; jk1516.
GeneIDi3433307.
KEGGicjk:jk1516.
PATRICi21513982. VBICorJei31838_1536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR931997 Genomic DNA. Translation: CAI37689.1.
RefSeqiWP_011273939.1. NC_007164.1.

3D structure databases

ProteinModelPortaliQ4JU18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi306537.jk1516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI37689; CAI37689; jk1516.
GeneIDi3433307.
KEGGicjk:jk1516.
PATRICi21513982. VBICorJei31838_1536.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0143. LUCA.
HOGENOMiHOG000200402.
KOiK01874.
OMAiFWPAMLD.
OrthoDBiPOG091H0269.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 2 hits.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_CORJK
AccessioniPrimary (citable) accession number: Q4JU18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 2, 2005
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.