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Q4JQX9

- DEN_VZVO

UniProt

Q4JQX9 - DEN_VZVO

Protein

Deneddylase

Gene

ORF22

Organism
Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei19 – 191Important for catalytic activityPROSITE-ProRule annotation
    Active sitei32 – 321PROSITE-ProRule annotation
    Active sitei168 – 1681PROSITE-ProRule annotation
    Active sitei170 – 1701PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: InterPro
    2. omega peptidase activity Source: InterPro

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Ubiquitin thioesterase
    Gene namesi
    ORF Names:ORF22
    OrganismiVaricella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
    Taxonomic identifieri341980 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000002603: Genome, UP000008504: Genome, UP000008505: Genome, UP000008506: Genome

    Subcellular locationi

    Virion tegument. Host cytoplasm. Host nucleus By similarity
    Note: Tightly associated with the capsid.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 27632763DeneddylasePRO_0000385479Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Protein-protein interaction databases

    IntActiQ4JQX9. 20 interactions.
    MINTiMINT-2826563.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 237226Peptidase C76PROSITE-ProRule annotationAdd
    BLAST
    Repeati2455 – 245731
    Repeati2458 – 246032
    Repeati2461 – 246333
    Repeati2464 – 246634
    Repeati2467 – 246935
    Repeati2470 – 247236
    Repeati2473 – 247537
    Repeati2476 – 247838

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 247247Deubiquitination activityBy similarityAdd
    BLAST
    Regioni2455 – 2478248 X 3 AA repeats of P-A/V-QAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi331 – 3377Nuclear localization signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi331 – 3355Poly-Arg
    Compositional biasi1367 – 13704Poly-Leu
    Compositional biasi2356 – 23594Poly-Leu

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR005210. Herpes_UL36.
    IPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    PF03586. Herpes_UL36. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4JQX9-1 [UniParc]FASTAAdd to Basket

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    MDIIPPIAVT VAGVGSRNQF DGALGPASGL SCLRTSLSFL HMTYAHGINA     50
    TLSSDMIDGC LQEGAAWTTD LSNMGRGVPD MCALVDLPNR ISYIKLGDTT 100
    STCCVLSRIY GDSHFFTVPD EGFMCTQIPA RAFFDDVWMG REESYTIITV 150
    DSTGMAIYRQ GNISFIFDPH GHGTIGQAVV VRVNTTDVYS YIASEYTHRP 200
    DNVESQWAAA LVFFVTANDG PVSEEALSSA VTLIYGSCDT YFTDEQYCEK 250
    LVTAQHPLLL SPPNSTTIVL NKSSIVPLHQ NVGESVSLEA TLHSTLTNTV 300
    ALDPRCSYSE VDPWHAVLET TSTGSGVLDC RRRRRPSWTP PSSEENLACI 350
    DDGLVNNTHS TDNLHKPAKK VLKFKPTVDV PDKTQVAHVL PRLREVANTP 400
    DVVLNVSNVD TPESSPTFSR NMNVGSSLKD RKPFLFEQSG DVNMVVEKLL 450
    QHGHEISNGY VQNAVGTLDT VITGHTNVPI WVTRPLVMPD EKDPLELFIN 500
    LTILRLTGFV VENGTRTHHG ATSVVSDFIG PLGEILTGFP SAAELIRVTS 550
    LILTNMPGAE YAIKTVLRKK CTIGMLIIAK FGLVAMRVQD TTGALHAELD 600
    VLEADLGGSS PIDLYSRLST GLISILNSPI ISHPGLFAEL IPTRTGSLSE 650
    RIRLLCELVS ARETRYMREH TALVSSVKAL ENALRSTRNK IDAIQIPEVP 700
    QEPPEETDIP PEELIRRVYE IRSEVTMLLT SAVTEYFTRG VLYSTRALIA 750
    EQSPRRFRVA TASTAPIQRL LDSLPEFDAK LTAIISSLSI HPPPETIQNL 800
    PVVSLLKELI KEGEDLNTDT ALVSWLSVVG EAQTAGYLSR REFDELSRTI 850
    KTINTRATQR ASAEAELSCF NTLSAAVDQA VKDYETYNNG EVKYPEITRD 900
    DLLATIVRAT DDLVRQIKIL SDPMIQSGLQ PSIKRRLETR LKEVQTYANE 950
    ARTTQDTIKS RKQAAYNKLG GLLRPVTGFV GLRAAVDLLP ELASELDVQG 1000
    ALVNLRTKVL EAPVEIRSQL TGDFWALFNQ YRDILEHPGN ARTSVLGGLG 1050
    ACFTAIIEIV PIPTEYRPSL LAFFGDVADV LASDIATVST NPESESAINA 1100
    VVATLSKATL VSSTVPALSF VLSLYKKYQA LQQEITNTHK LTELQKQLGD 1150
    DFSTLAVSSG HLKFISSSNV DDYEINDAIL SIQTNVHALM DTVKLVEVEL 1200
    QKLPPHCIAG TSTLSRVVKD LHKLVTMAHE KKEQAKVLIT DCERAHKQQT 1250
    TRVLYERWTR DIIACLEAME TRHVFNGTEL ARLRDMAAAG GFDIHAVYPQ 1300
    ARQVVAACET TAVTALDTVF RHNPHTPENT NIPPPLALLR GLTWFDDFSI 1350
    TAPVFTVMFP GVSIEGLLLL MRIRAVVLLS ADTSINGIPN YRDMILRTSG 1400
    DLLQIPALAG YVDFYTRSYD QFITESVTLS ELRADIRQAA GAKLTEANKA 1450
    LEEVTHVRAH ETAKLALKEG VFITLPSEGL LIRAIEYFTT FDHKRFIGTA 1500
    YERVLQTMVD RDLKEANAEL AQFRMVCQAT KNRAIQILQN IVDTANATEQ 1550
    QEDVDFTNLK TLLKLTPPPK TIALAIDRST SVQDIVTQFA LLLGRLEEET 1600
    GTLDIQAVDW MYQARNIIDS HPLSVRIDGT GPLHTYKDRV DKLYALRTKL 1650
    DLLRRRIETG EVTWDDAWTT FKRETGDMLA SGDTYATSVD SIKALQASAS 1700
    VVDMLCSEPE FFLLPVETKN RLQKKQQERK TALDVVLQKQ RQFEETASRL 1750
    RALIERIPTE SDHDVLRMLL HDFDQFTHLP IWIKTQYMTF RNLLMVRLGL 1800
    YASYAEIFPP ASPNGVFAPI PAMSGVCLED QSRCIRARVA AFMGEASVVQ 1850
    TFREARSSID ALFGKNLTFY LDTDGVPLRY RVCYKSVGVK LGTMLCSQGG 1900
    LSLRPALPDE GIVEETTLSA LRVANEVNEL RIEYESAIKS GFSAFSTFVR 1950
    HRHAEWGKTN ARRAIAEIYA GLITTTLTRQ YGVHWDKLIY SFEKHHLTSV 2000
    MGNGLTKPIQ RRGDVRVLEL TLSDIVTILV ATTPVHLLNF ARLDLIKQHE 2050
    YMARTLRPVI EAAFRGRLLV RSLDGDPKGN ARAFFNAAPS KHKLPLALGS 2100
    NQDPTGGRIF AFRMADWKLV KMPQKITDPF APWQLSPPPG VKANVDAVTR 2150
    IMATDRLATI TVLGRMCLPP ISLVSMWNTL QPEEFAYRTQ DDVDIIVDAR 2200
    LDLSSTLNAR FDTAPSNTTL EWNTDRKVIT DAYIQTGATT VFTVTGAAPT 2250
    HVSNVTAFDI ATTAILFGAP LVIAMELTSV FSQNSGLTLG LKLFDSRHMA 2300
    TDSGISSAVS PDIVSWGLRL LHMDPHPIEN ACLIVQLEKL SALIANKPLT 2350
    NNPPCLLLLD EHMNPSYVLW ERKDSIPAPD YVVFWGPESL IDLPYIDSDE 2400
    DSFPSCPDDP FYSQIIAGYA PQGPPNLDTT DFYPTEPLFK SPVQVVRSSK 2450
    CKKMPVQPVQ PAQPVQPAQP AQPVQPAQPI EPGTQIVVQN FKKPQSVKTT 2500
    LSQKDIPLYV ETESETAVLI PKQLTTSIKT TVCKSITPPN NQLSDWKNNP 2550
    QQNQTLNQAF NKPILEITSI PTDDSISYRT WIEKSNQTQK RHQNDPRMYN 2600
    SKTVFHPVNN QLPSWVDTAA DAPQTDLLTN YKTRQPSPNF PRDVHTWGVS 2650
    SNPFNSPNRD LYESDFSEPS DGYSSESENS IVLSLDEHRS CRVPRHVRVV 2700
    NADVVTGRRY VRGTALGALA LLSQACRRMI DNVRYTRKLL MDHTEDIFQG 2750
    LGYVKLLLDG TYI 2763
    Length:2,763
    Mass (Da):306,336
    Last modified:September 22, 2009 - v2
    Checksum:iC0E4F5AC0A599529
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti2468 – 24681A → V in strain: Oka varicella vaccine VarilRix (V-Oka-GSK).

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB097932 Genomic DNA. No translation available.
    AB097933 Genomic DNA. No translation available.
    DQ008355 Genomic DNA. Translation: AAY57708.1.
    DQ008354 Genomic DNA. Translation: AAY57637.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB097932 Genomic DNA. No translation available.
    AB097933 Genomic DNA. No translation available.
    DQ008355 Genomic DNA. Translation: AAY57708.1 .
    DQ008354 Genomic DNA. Translation: AAY57637.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q4JQX9. 20 interactions.
    MINTi MINT-2826563.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR005210. Herpes_UL36.
    IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    PF03586. Herpes_UL36. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the complete DNA sequences of the Oka varicella vaccine and its parental virus."
      Gomi Y., Sunamachi H., Mori Y., Nagaike K., Takahashi M., Yamanishi K.
      J. Virol. 76:11447-11459(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Isolate Human/Japan/P-Oka/1970 and Oka varicella vaccine Biken (V-Oka-Biken).
    2. "Complete DNA sequences of two oka strain varicella-zoster virus genomes."
      Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M., Vassilev V.
      J. Virol. 82:11023-11044(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Oka varicella vaccine VarilRix (V-Oka-GSK) and Oka varicella vaccine Varivax (V-Oka-Merk).

    Entry informationi

    Entry nameiDEN_VZVO
    AccessioniPrimary (citable) accession number: Q4JQX9
    Secondary accession number(s): Q4JQV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3