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Protein

Thymidylate synthase

Gene

ORF13

Organism
Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of deoxyuridylate to thymidylate.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei183 – 1831By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
ORF Names:ORF13
OrganismiVaricella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri341980 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008505 Componenti: Genome
  • UP000008504 Componenti: Genome
  • UP000002603 Componenti: Genome
  • UP000008506 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Thymidylate synthasePRO_0000385165Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ4JQW2. 2 interactions.

Structurei

Secondary structure

1
301
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 2810Combined sources
Beta strandi33 – 364Combined sources
Beta strandi42 – 465Combined sources
Beta strandi49 – 546Combined sources
Beta strandi56 – 583Combined sources
Turni63 – 664Combined sources
Helixi69 – 8113Combined sources
Helixi87 – 904Combined sources
Turni91 – 933Combined sources
Turni98 – 1014Combined sources
Helixi103 – 1064Combined sources
Turni107 – 1104Combined sources
Helixi123 – 1286Combined sources
Beta strandi144 – 1463Combined sources
Helixi148 – 15811Combined sources
Beta strandi166 – 1683Combined sources
Turni172 – 1743Combined sources
Helixi175 – 1773Combined sources
Beta strandi178 – 1803Combined sources
Beta strandi183 – 19210Combined sources
Beta strandi195 – 20612Combined sources
Turni207 – 2093Combined sources
Helixi210 – 22819Combined sources
Beta strandi232 – 24615Combined sources
Helixi249 – 2568Combined sources
Beta strandi266 – 2694Combined sources
Helixi276 – 2783Combined sources
Helixi281 – 2833Combined sources
Beta strandi284 – 2874Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4XSCX-ray2.90A/B/C/D8-295[»]
4XSDX-ray2.90A/B/C/D8-295[»]
4XSEX-ray3.10A/B/C/D8-295[»]
ProteinModelPortaliQ4JQW2.
SMRiQ4JQW2. Positions 17-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

KOiK00560.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JQW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDLSCWTKV PGFTLTGELQ YLKQVDDILR YGVRKRDRTG IGTLSLFGMQ
60 70 80 90 100
ARYNLRNEFP LLTTKRVFWR AVVEELLWFI RGSTDSKELA AKDIHIWDIY
110 120 130 140 150
GSSKFLNRNG FHKRHTGDLG PIYGFQWRHF GAEYKDCQSN YLQQGIDQLQ
160 170 180 190 200
TVIDTIKTNP ESRRMIISSW NPKDIPLMVL PPCHTLCQFY VANGELSCQV
210 220 230 240 250
YQRSGDMGLG VPFNIAGYAL LTYIVAHVTG LKTGDLIHTM GDAHIYLNHI
260 270 280 290 300
DALKVQLARS PKPFPCLKII RNVTDINDFK WDDFQLDGYN PHPPLKMEMA

L
Length:301
Mass (Da):34,533
Last modified:March 24, 2009 - v1
Checksum:i8B84C36E79F95861
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB097932 Genomic DNA. No translation available.
AB097933 Genomic DNA. No translation available.
DQ008354 Genomic DNA. Translation: AAY57630.1.
DQ008355 Genomic DNA. Translation: AAY57701.1.
RefSeqiNP_040136.1. NC_001348.1.

Genome annotation databases

GeneIDi1487659.
KEGGivg:1487659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB097932 Genomic DNA. No translation available.
AB097933 Genomic DNA. No translation available.
DQ008354 Genomic DNA. Translation: AAY57630.1.
DQ008355 Genomic DNA. Translation: AAY57701.1.
RefSeqiNP_040136.1. NC_001348.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4XSCX-ray2.90A/B/C/D8-295[»]
4XSDX-ray2.90A/B/C/D8-295[»]
4XSEX-ray3.10A/B/C/D8-295[»]
ProteinModelPortaliQ4JQW2.
SMRiQ4JQW2. Positions 17-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ4JQW2. 2 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1487659.
KEGGivg:1487659.

Phylogenomic databases

KOiK00560.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparison of the complete DNA sequences of the Oka varicella vaccine and its parental virus."
    Gomi Y., Sunamachi H., Mori Y., Nagaike K., Takahashi M., Yamanishi K.
    J. Virol. 76:11447-11459(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate Human/Japan/P-Oka/1970 and Oka varicella vaccine Biken (V-Oka-Biken).
  2. "Complete DNA sequences of two oka strain varicella-zoster virus genomes."
    Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M., Vassilev V.
    J. Virol. 82:11023-11044(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Oka varicella vaccine VarilRix (V-Oka-GSK) and Oka varicella vaccine Varivax (V-Oka-Merk).

Entry informationi

Entry nameiTYSY_VZVO
AccessioniPrimary (citable) accession number: Q4JQW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 24, 2009
Last modified: March 16, 2016
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.