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Protein

Thymidylate synthase

Gene

ORF13

Organism
Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of deoxyuridylate to thymidylate.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38dUMPBy similarity1
Active sitei183NucleophileBy similarity1
Binding sitei2065,10-methylenetetrahydrofolateBy similarity1
Binding sitei214dUMPBy similarity1
Binding sitei3005,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi163 – 164dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi203 – 206dUMPBy similarity4
Nucleotide bindingi244 – 246dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
ORF Names:ORF13
OrganismiVaricella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri341980 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008505 Componenti: Genome
  • UP000008504 Componenti: Genome
  • UP000002603 Componenti: Genome
  • UP000008506 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003851651 – 301Thymidylate synthaseAdd BLAST301

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ4JQW2. 2 interactors.

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 28Combined sources10
Beta strandi33 – 36Combined sources4
Beta strandi42 – 46Combined sources5
Beta strandi49 – 54Combined sources6
Beta strandi56 – 58Combined sources3
Turni63 – 66Combined sources4
Helixi69 – 81Combined sources13
Helixi87 – 90Combined sources4
Turni91 – 93Combined sources3
Turni98 – 101Combined sources4
Helixi103 – 106Combined sources4
Turni107 – 110Combined sources4
Helixi123 – 128Combined sources6
Beta strandi144 – 146Combined sources3
Helixi148 – 158Combined sources11
Beta strandi166 – 168Combined sources3
Turni172 – 174Combined sources3
Helixi175 – 177Combined sources3
Beta strandi178 – 180Combined sources3
Beta strandi183 – 192Combined sources10
Beta strandi195 – 206Combined sources12
Turni207 – 209Combined sources3
Helixi210 – 228Combined sources19
Beta strandi232 – 246Combined sources15
Helixi249 – 256Combined sources8
Beta strandi266 – 269Combined sources4
Helixi276 – 278Combined sources3
Helixi281 – 283Combined sources3
Beta strandi284 – 287Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XSCX-ray2.90A/B/C/D8-295[»]
4XSDX-ray2.90A/B/C/D8-295[»]
4XSEX-ray3.10A/B/C/D8-295[»]
ProteinModelPortaliQ4JQW2.
SMRiQ4JQW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

KOiK00560.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JQW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDLSCWTKV PGFTLTGELQ YLKQVDDILR YGVRKRDRTG IGTLSLFGMQ
60 70 80 90 100
ARYNLRNEFP LLTTKRVFWR AVVEELLWFI RGSTDSKELA AKDIHIWDIY
110 120 130 140 150
GSSKFLNRNG FHKRHTGDLG PIYGFQWRHF GAEYKDCQSN YLQQGIDQLQ
160 170 180 190 200
TVIDTIKTNP ESRRMIISSW NPKDIPLMVL PPCHTLCQFY VANGELSCQV
210 220 230 240 250
YQRSGDMGLG VPFNIAGYAL LTYIVAHVTG LKTGDLIHTM GDAHIYLNHI
260 270 280 290 300
DALKVQLARS PKPFPCLKII RNVTDINDFK WDDFQLDGYN PHPPLKMEMA

L
Length:301
Mass (Da):34,533
Last modified:March 24, 2009 - v1
Checksum:i8B84C36E79F95861
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB097932 Genomic DNA. No translation available.
AB097933 Genomic DNA. No translation available.
DQ008354 Genomic DNA. Translation: AAY57630.1.
DQ008355 Genomic DNA. Translation: AAY57701.1.
RefSeqiNP_040136.1. NC_001348.1.

Genome annotation databases

GeneIDi1487659.
KEGGivg:1487659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB097932 Genomic DNA. No translation available.
AB097933 Genomic DNA. No translation available.
DQ008354 Genomic DNA. Translation: AAY57630.1.
DQ008355 Genomic DNA. Translation: AAY57701.1.
RefSeqiNP_040136.1. NC_001348.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XSCX-ray2.90A/B/C/D8-295[»]
4XSDX-ray2.90A/B/C/D8-295[»]
4XSEX-ray3.10A/B/C/D8-295[»]
ProteinModelPortaliQ4JQW2.
SMRiQ4JQW2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ4JQW2. 2 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1487659.
KEGGivg:1487659.

Phylogenomic databases

KOiK00560.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_VZVO
AccessioniPrimary (citable) accession number: Q4JQW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 24, 2009
Last modified: November 30, 2016
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.