Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q4JQV6

- RIR1_VZVO

UniProt

Q4JQV6 - RIR1_VZVO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

ORF19

Organism
Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. The N-terminal region confers antiapoptotic activity in differentiated cells such as neurons and is important for viral reactivation to increase neural survivability By similarity.By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei200 – 2001SubstrateBy similarity
Sitei216 – 2161Important for hydrogen atom transferBy similarity
Binding sitei246 – 2461Substrate; via amide nitrogenBy similarity
Active sitei427 – 4271Proton acceptorBy similarity
Active sitei429 – 4291Cysteine radical intermediateBy similarity
Active sitei431 – 4311Proton acceptorBy similarity
Sitei444 – 4441Important for hydrogen atom transferBy similarity
Sitei750 – 7501Important for electron transferBy similarity
Sitei751 – 7511Important for electron transferBy similarity
Sitei770 – 7701Interacts with thioredoxin/glutaredoxinBy similarity
Sitei773 – 7731Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Short name:
R1
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:ORF19
OrganismiVaricella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri341980 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002603: Genome, UP000008504: Genome, UP000008505: Genome, UP000008506: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 775775Ribonucleoside-diphosphate reductase large subunitPRO_0000385163Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi216 ↔ 444Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit ORF19 (R1) and a homodimer of the small subunit ORF18 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

Protein-protein interaction databases

IntActiQ4JQV6. 20 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ4JQV6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni215 – 2162Substrate bindingBy similarity
Regioni427 – 4315Substrate bindingBy similarity
Regioni606 – 6105Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JQV6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFKRIFNTV HDIINRLCQH GYKEYIIPPE STTPVELMEY ISTIVSKLKA
60 70 80 90 100
VTRQDERVYR CCGELIHCRI NLRSVSMETW LTSPILCLTP RVRQAIEGRR
110 120 130 140 150
DEIRRAILEP FLKDQYPALA TLGLQSALKY EDFYLTKLEE GKLESLCQFF
160 170 180 190 200
LRLAATVTTE IVNLPKIATL IPGINDGYTW TDVCRVFFTA LACQKIVPAT
210 220 230 240 250
PVMMFLGRET GATASCYLMD PESITVGRAV RAITGDVGTV LQSRGGVGIS
260 270 280 290 300
LQSLNLIPTE NQTKGLLAVL KLLDCMVMAI NSDCERPTGV CVYIEPWHVD
310 320 330 340 350
LQTVLATRGM LVRDEIFRCD NIFCCLWTPD LFFERYLSYL KGASNVQWTL
360 370 380 390 400
FDNRADILRT LHGEAFTSTY LRLEREGLGV SSVPIQDIAF TIIRSAAVTG
410 420 430 440 450
SPFLMFKDAC NRNYHMNTQG NAITGSNLCT EIVQKADAHQ HGVCNLASIN
460 470 480 490 500
LTTCLSKGPV SFNLNDLQLT ARTTVIFLNG VLAAGNFPCK KSCKGVKNNR
510 520 530 540 550
SLGIGIQGLH TTCLRLGFDL TSQPARRLNV QIAELMLYET MKTSMEMCKI
560 570 580 590 600
GGLAPFKGFT ESKYAKGWLH QDGFSTISYL DLPWCTLRDD ICAYGLYNSQ
610 620 630 640 650
FLALMPTVSS AQVTECSEGF SPIYNNMFSK VTTSGELLRP NLDLMDELRD
660 670 680 690 700
MYSCEEKRLE VINILEKNQW SVIRSFGCLS NSHPLLKYKT AFEYEQEDLV
710 720 730 740 750
DMCAERAPFI DQSQSMTLFI EERPDGTIPA SKIMNLLIRA YKAGLKTGMY
760 770
YCKIRKATNS GLFAGGELTC TSCAL
Length:775
Mass (Da):86,828
Last modified:March 24, 2009 - v1
Checksum:i187695A65EB91B9D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB097932 Genomic DNA. No translation available.
AB097933 Genomic DNA. No translation available.
DQ008354 Genomic DNA. Translation: AAY57685.1.
DQ008355 Genomic DNA. Translation: AAY57756.1.
RefSeqiNP_040142.1. NC_001348.1.

Genome annotation databases

GeneIDi1487716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB097932 Genomic DNA. No translation available.
AB097933 Genomic DNA. No translation available.
DQ008354 Genomic DNA. Translation: AAY57685.1 .
DQ008355 Genomic DNA. Translation: AAY57756.1 .
RefSeqi NP_040142.1. NC_001348.1.

3D structure databases

ProteinModelPortali Q4JQV6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q4JQV6. 20 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1487716.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Comparison of the complete DNA sequences of the Oka varicella vaccine and its parental virus."
    Gomi Y., Sunamachi H., Mori Y., Nagaike K., Takahashi M., Yamanishi K.
    J. Virol. 76:11447-11459(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate Human/Japan/P-Oka/1970 and Oka varicella vaccine Biken (V-Oka-Biken).
  2. "Complete DNA sequences of two oka strain varicella-zoster virus genomes."
    Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M., Vassilev V.
    J. Virol. 82:11023-11044(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Oka varicella vaccine VarilRix (V-Oka-GSK) and Oka varicella vaccine Varivax (V-Oka-Merk).
  3. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_VZVO
AccessioniPrimary (citable) accession number: Q4JQV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 24, 2009
Last modified: October 1, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3