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Protein

Phosphatidylcholine:ceramide cholinephosphotransferase 2

Gene

Sgms2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity (By similarity).By similarity

Catalytic activityi

A ceramide + a phosphatidylcholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei229 – 2291By similarity
Active sitei272 – 2721By similarity
Active sitei276 – 2761By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi2.7.8.27. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylcholine:ceramide cholinephosphotransferase 2 (EC:2.7.8.27)
Alternative name(s):
Sphingomyelin synthase 2
Gene namesi
Name:Sgms2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305778. Sgms2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Transmembranei128 – 14821HelicalSequence analysisAdd
BLAST
Transmembranei159 – 17921HelicalSequence analysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence analysisAdd
BLAST
Transmembranei248 – 26821HelicalSequence analysisAdd
BLAST
Transmembranei273 – 29018HelicalSequence analysisAdd
BLAST
Topological domaini291 – 36575CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of cell outer membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Phosphatidylcholine:ceramide cholinephosphotransferase 2PRO_0000290123Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi331 – 3311S-palmitoyl cysteineBy similarity
Lipidationi332 – 3321S-palmitoyl cysteineBy similarity
Lipidationi343 – 3431S-palmitoyl cysteineBy similarity
Lipidationi348 – 3481S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization.By similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ4JM44.

Expressioni

Tissue specificityi

Expression restricted to late round spermatids and elongating spermatids but not detected in late elongate spermatids and Sertoli cells (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000011284.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015020.

Family & Domainsi

Sequence similaritiesi

Belongs to the sphingomyelin synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3058. Eukaryota.
ENOG410XNSC. LUCA.
HOGENOMiHOG000233822.
HOVERGENiHBG048216.
InParanoidiQ4JM44.
KOiK04714.
PhylomeDBiQ4JM44.
TreeFamiTF314547.

Family and domain databases

InterProiIPR025749. Sphingomyelin_synth-like_dom.
[Graphical view]
PfamiPF14360. PAP2_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JM44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIIETAKLE GHLESQTNNS TNTYTSPTEA VEEEDKNGKG KPKTLSNGLR
60 70 80 90 100
KGAKKYPDYI QISMPNDSRN KLPLEWWKTG IAFVYALFNL ILTTVMITVV
110 120 130 140 150
HERVPPKELS PPLPDKFFDY VDRVKWAFSV SEINGMVLVG LWLTQWLFLR
160 170 180 190 200
YKSIVGRRFF FIMGTLYLYR CITMYVTTLP VPGMHFQCAP KLNGDSQAKI
210 220 230 240 250
QRILRLLSGG GLSITGSHIL CGDFLFSGHT VVLTLTYLFI KEYSPRHFWW
260 270 280 290 300
YHLVCWLLSA AGIICILVAH EHYTVDVIIA YYITTRLFWW YHSMANEKNL
310 320 330 340 350
KVSSQTNFLS RAWWFPIFYF FEKNVQGSIP CCFSWPLSWP PGCFKSSCKK
360
YSRVQKIGED NEKST
Length:365
Mass (Da):42,223
Last modified:April 18, 2006 - v2
Checksum:iA3BD73AFF11245D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti264 – 2641I → V in AAH85803 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ071571 mRNA. Translation: AAY84706.2.
BC085803 mRNA. Translation: AAH85803.1.
RefSeqiNP_001014065.1. NM_001014043.1.
XP_006233373.1. XM_006233311.2.
XP_008759723.1. XM_008761501.1.
UniGeneiRn.161863.

Genome annotation databases

GeneIDi310849.
KEGGirno:310849.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ071571 mRNA. Translation: AAY84706.2.
BC085803 mRNA. Translation: AAH85803.1.
RefSeqiNP_001014065.1. NM_001014043.1.
XP_006233373.1. XM_006233311.2.
XP_008759723.1. XM_008761501.1.
UniGeneiRn.161863.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015020.

Proteomic databases

PaxDbiQ4JM44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi310849.
KEGGirno:310849.

Organism-specific databases

CTDi166929.
RGDi1305778. Sgms2.

Phylogenomic databases

eggNOGiKOG3058. Eukaryota.
ENOG410XNSC. LUCA.
HOGENOMiHOG000233822.
HOVERGENiHBG048216.
InParanoidiQ4JM44.
KOiK04714.
PhylomeDBiQ4JM44.
TreeFamiTF314547.

Enzyme and pathway databases

BRENDAi2.7.8.27. 5301.

Miscellaneous databases

PROiQ4JM44.

Gene expression databases

BgeeiENSRNOG00000011284.

Family and domain databases

InterProiIPR025749. Sphingomyelin_synth-like_dom.
[Graphical view]
PfamiPF14360. PAP2_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMS2_RAT
AccessioniPrimary (citable) accession number: Q4JM44
Secondary accession number(s): Q5U2Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: April 18, 2006
Last modified: September 7, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.