Q4JCP0 (NADE_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Saci_0009

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152234
Regions
	Nucleotide binding	39 – 46	8	ATP By similarity
Sites
	Active site	41	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JCP0 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: E34A4D7FD8C9A5BE	FASTA	279	31,593
	10 20 30 40 50 60 MPEYVREKLT LNFPLVTEYL VKRIKDYINN SGKSGGIIGL SGGIDSSVAS VLLSKATENF 70 80 90 100 110 120 HVLLMPSSST PKEDLDHAFM ILRLINATES KYTIINIDPI VDQFRLAVKT NDKIISGNIK 130 140 150 160 170 180 ARSRMILLYA FAQKFNYLVV GTGDKSELML GYFTKYGDGG VDILPLGDLY KTQVRMLGRY 190 200 210 220 230 240 LGVPEDIVKK PPSPALWEGQ TAEGEIGLDY ETIDSILYLR FEEMRSENEI SALVNVPIDL 250 260 270 VRRIVRMVKI SQHKRLPPEI FRLSGRSINS DWRYPRQWA

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY79439.1.
RefSeq	YP_254732.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4JCP0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3473294.
GenomeReviews	Gene locus Saci_0009 in contig CP000077_GR.
KEGG	sai:Saci_0009.
NMPDR	fig|330779.3.peg.1749.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_0009-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_SULAC

Accession

Primary (citable) accession number: Q4JCP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 2, 2005
Last modified:	November 16, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program