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Q4JCJ1

- CAPPA_SULAC

UniProt

Q4JCJ1 - CAPPA_SULAC

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Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.1 PublicationUniRule annotation

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.1 PublicationUniRule annotation

Cofactori

Mg2+1 PublicationUniRule annotationNote: Mg(2+) cannot be replaced by Mn(2+).1 PublicationUniRule annotation

Enzyme regulationi

Allosterically inhibited by L-aspartate and L-malate. PEPC activity is not affected by allosteric activators of E.coli PEPC such as glucose 6-phosphate, fructose 1,6-bisphosphate, and acetyl coenzyme A.1 Publication

Kineticsi

  1. KM=0.20 mM for phosphoenolpyruvate1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 90 degrees Celsius. Is extremely thermostable. No loss of activity is observed after incubation for 2 hours at 80 degrees Celsius. The times required for 50% loss of activity are about 60 minutes at 90 degrees Celsius, 10 minutes at 95 degrees Celsius, and 1 minute at 100 degrees Celsius.1 Publication

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB
  2. phosphoenolpyruvate carboxylase activity Source: UniProtKB

GO - Biological processi

  1. carbon fixation Source: UniProtKB
  2. oxaloacetate metabolic process Source: UniProtKB
  3. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbon dioxide fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciSACI330779:GH9J-59-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylaseUniRule annotation (EC:4.1.1.31UniRule annotation)
Short name:
PEPCUniRule annotation
Short name:
PEPCaseUniRule annotation
Gene namesi
Name:ppcAUniRule annotation
Ordered Locus Names:Saci_0059
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001018: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Phosphoenolpyruvate carboxylasePRO_0000309614Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 PublicationUniRule annotation

Protein-protein interaction databases

STRINGi330779.Saci_0059.

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1892.
HOGENOMiHOG000038601.
KOiK01595.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006677. UCP006677. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4JCJ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKIPRTMST QHPDNAKVPE WNQGEAISGE NEIIEAYLAF SRYGVEEVMW
60 70 80 90 100
DAEGKDVDTH VVRKLLSQYP EFFRHRILGK DIFLTYRVPN PKIEGAERKV
110 120 130 140 150
FAETLNTIPI TYDLAEKFYG ENPNPPVFEV ILPFTTSYEE LIAVIKFYEK
160 170 180 190 200
VIVNSDNTKL VDDTYVKDII GETNPKKIEV IPLIEDRDSM LRIDTIVGKY
210 220 230 240 250
IEIERPPYLR VFLARSDPAM NYGLLSAVLS VKYALSRLSK MEKIYGVKIF
260 270 280 290 300
PLLGVGSLPF RGHFSPYNVE NTLNEYRGIY TFTVQSAFKY DYEDDLVISA
310 320 330 340 350
IKKVNGTNVT EKIELSEEDE EIISNVTRKY TQGYQNKIEA LADVINKVAL
360 370 380 390 400
LLPRRRARKL HIGLFGYSRS TGKVTLPRAI SFVGSLYTIG IPPEIIGLSS
410 420 430 440 450
LSKMTDQELN AIFNNYKYLK NDLQFAARFV NFEALQLLKD IWNIDAEVVK
460 470 480 490 500
AIKEDIDYAE NSLGIRIGES DYMSKKHVLL STLALLSIKE GKLDEAKTYI
510
KEMAIVRRAI G
Length:511
Mass (Da):58,355
Last modified:November 13, 2007 - v2
Checksum:i1337C9AF4012A917
GO

Sequence cautioni

The sequence AAY79488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79488.1. Different initiation.
RefSeqiYP_254781.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79488; AAY79488; Saci_0059.
GeneIDi3472974.
KEGGisai:Saci_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79488.1 . Different initiation.
RefSeqi YP_254781.1. NC_007181.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 330779.Saci_0059.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY79488 ; AAY79488 ; Saci_0059 .
GeneIDi 3472974.
KEGGi sai:Saci_0059.

Phylogenomic databases

eggNOGi COG1892.
HOGENOMi HOG000038601.
KOi K01595.

Enzyme and pathway databases

BioCyci SACI330779:GH9J-59-MONOMER.

Family and domain databases

HAMAPi MF_01904. PEPcase_type2.
InterProi IPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
Pfami PF14010. PEPcase_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006677. UCP006677. 1 hit.
SUPFAMi SSF51621. SSF51621. 1 hit.
TIGRFAMsi TIGR02751. PEPCase_arch. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
    Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
    J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
  2. "Biochemical relationship of phosphoenolpyruvate carboxylases (PEPCs) from thermophilic archaea."
    Sako Y., Takai K., Nishizaka T., Ishida Y.
    FEMS Microbiol. Lett. 153:159-165(1997)
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Entry informationi

Entry nameiCAPPA_SULAC
AccessioniPrimary (citable) accession number: Q4JCJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3