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Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.UniRule annotation1 Publication

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 PublicationNote: Mg(2+) cannot be replaced by Mn2+.UniRule annotation1 Publication

Enzyme regulationi

Allosterically inhibited by L-aspartate and L-malate. PEPC activity is not affected by allosteric activators of E.coli PEPC such as glucose 6-phosphate, fructose 1,6-bisphosphate, and acetyl coenzyme A.1 Publication

Kineticsi

  1. KM=0.20 mM for phosphoenolpyruvate1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Temperature dependencei

    Optimum temperature is 90 degrees Celsius. Is extremely thermostable. No loss of activity is observed after incubation for 2 hours at 80 degrees Celsius. The times required for 50% loss of activity are about 60 minutes at 90 degrees Celsius, 10 minutes at 95 degrees Celsius, and 1 minute at 100 degrees Celsius.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciSACI330779:GH9J-59-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylaseUniRule annotation (EC:4.1.1.31UniRule annotation)
    Short name:
    PEPCUniRule annotation
    Short name:
    PEPCaseUniRule annotation
    Gene namesi
    Name:ppcAUniRule annotation
    Ordered Locus Names:Saci_0059
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001018 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 511511Phosphoenolpyruvate carboxylasePRO_0000309614Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi330779.Saci_0059.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1892.
    HOGENOMiHOG000038601.
    KOiK01595.

    Family and domain databases

    HAMAPiMF_01904. PEPcase_type2.
    InterProiIPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF14010. PEPcase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006677. UCP006677. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4JCJ1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRKIPRTMST QHPDNAKVPE WNQGEAISGE NEIIEAYLAF SRYGVEEVMW
    60 70 80 90 100
    DAEGKDVDTH VVRKLLSQYP EFFRHRILGK DIFLTYRVPN PKIEGAERKV
    110 120 130 140 150
    FAETLNTIPI TYDLAEKFYG ENPNPPVFEV ILPFTTSYEE LIAVIKFYEK
    160 170 180 190 200
    VIVNSDNTKL VDDTYVKDII GETNPKKIEV IPLIEDRDSM LRIDTIVGKY
    210 220 230 240 250
    IEIERPPYLR VFLARSDPAM NYGLLSAVLS VKYALSRLSK MEKIYGVKIF
    260 270 280 290 300
    PLLGVGSLPF RGHFSPYNVE NTLNEYRGIY TFTVQSAFKY DYEDDLVISA
    310 320 330 340 350
    IKKVNGTNVT EKIELSEEDE EIISNVTRKY TQGYQNKIEA LADVINKVAL
    360 370 380 390 400
    LLPRRRARKL HIGLFGYSRS TGKVTLPRAI SFVGSLYTIG IPPEIIGLSS
    410 420 430 440 450
    LSKMTDQELN AIFNNYKYLK NDLQFAARFV NFEALQLLKD IWNIDAEVVK
    460 470 480 490 500
    AIKEDIDYAE NSLGIRIGES DYMSKKHVLL STLALLSIKE GKLDEAKTYI
    510
    KEMAIVRRAI G
    Length:511
    Mass (Da):58,355
    Last modified:November 13, 2007 - v2
    Checksum:i1337C9AF4012A917
    GO

    Sequence cautioni

    The sequence AAY79488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79488.1. Different initiation.
    RefSeqiWP_011276989.1. NC_007181.1.
    YP_254781.1. NC_007181.1.

    Genome annotation databases

    EnsemblBacteriaiAAY79488; AAY79488; Saci_0059.
    GeneIDi3472974.
    KEGGisai:Saci_0059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79488.1. Different initiation.
    RefSeqiWP_011276989.1. NC_007181.1.
    YP_254781.1. NC_007181.1.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi330779.Saci_0059.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAY79488; AAY79488; Saci_0059.
    GeneIDi3472974.
    KEGGisai:Saci_0059.

    Phylogenomic databases

    eggNOGiCOG1892.
    HOGENOMiHOG000038601.
    KOiK01595.

    Enzyme and pathway databases

    BioCyciSACI330779:GH9J-59-MONOMER.

    Family and domain databases

    HAMAPiMF_01904. PEPcase_type2.
    InterProiIPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF14010. PEPcase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006677. UCP006677. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
      Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
      J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
    2. "Biochemical relationship of phosphoenolpyruvate carboxylases (PEPCs) from thermophilic archaea."
      Sako Y., Takai K., Nishizaka T., Ishida Y.
      FEMS Microbiol. Lett. 153:159-165(1997)
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

    Entry informationi

    Entry nameiCAPPA_SULAC
    AccessioniPrimary (citable) accession number: Q4JCJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: November 13, 2007
    Last modified: June 24, 2015
    This is version 50 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.