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Q4JC68

- XPD_SULAC

UniProt

Q4JC68 - XPD_SULAC

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Protein

ATP-dependent DNA helicase Saci_0192

Gene

Saci_0192

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

ATP-dependent 5'-3' DNA helicase involved in nucleotide excision repair (NER) of DNA.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron-sulfur (4Fe-4S)
Metal bindingi102 – 1021Iron-sulfur (4Fe-4S)
Metal bindingi105 – 1051Iron-sulfur (4Fe-4S)
Metal bindingi137 – 1371Iron-sulfur (4Fe-4S)

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 368ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB
  2. 5'-3' DNA helicase activity Source: UniProtKB
  3. ATP binding Source: UniProtKB-KW
  4. ATP-dependent DNA helicase activity Source: InterPro
  5. DNA binding Source: UniProtKB-KW
  6. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA duplex unwinding Source: UniProtKB
  2. DNA repair Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription

Keywords - Ligandi

4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSACI330779:GH9J-191-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Saci_0192 (EC:3.6.4.12)
Gene namesi
Ordered Locus Names:Saci_0192
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001018: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351K → A: Abolishes helicase activity but not iron-sulfur-binding. 1 Publication
Mutagenesisi84 – 841K → H: Impairs iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi88 – 881C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi102 – 1021C → S: Does not affect iron-sulfur-binding nor helicase activity. 1 Publication
Mutagenesisi105 – 1051C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi136 – 1361F → P: Impairs iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi137 – 1371C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551ATP-dependent DNA helicase Saci_0192PRO_0000352309Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi330779.Saci_0192.

Structurei

Secondary structure

1
551
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2015Combined sources
Beta strandi24 – 285Combined sources
Helixi35 – 4612Combined sources
Beta strandi48 – 569Combined sources
Helixi57 – 593Combined sources
Helixi60 – 678Combined sources
Beta strandi78 – 803Combined sources
Helixi84 – 874Combined sources
Helixi97 – 993Combined sources
Helixi102 – 1043Combined sources
Turni106 – 1094Combined sources
Helixi120 – 13415Combined sources
Helixi138 – 1458Combined sources
Helixi146 – 1483Combined sources
Beta strandi150 – 1556Combined sources
Helixi157 – 1604Combined sources
Helixi162 – 1654Combined sources
Beta strandi175 – 1795Combined sources
Helixi182 – 19211Combined sources
Beta strandi194 – 1963Combined sources
Helixi198 – 20710Combined sources
Helixi211 – 22414Combined sources
Helixi245 – 26420Combined sources
Helixi272 – 28514Combined sources
Beta strandi289 – 2935Combined sources
Beta strandi296 – 3005Combined sources
Helixi305 – 3084Combined sources
Helixi309 – 3124Combined sources
Beta strandi317 – 3248Combined sources
Helixi328 – 3336Combined sources
Beta strandi341 – 3455Combined sources
Helixi346 – 3494Combined sources
Beta strandi357 – 3637Combined sources
Turni370 – 3723Combined sources
Helixi375 – 39117Combined sources
Beta strandi393 – 4019Combined sources
Helixi403 – 4108Combined sources
Beta strandi415 – 4195Combined sources
Helixi426 – 4327Combined sources
Beta strandi435 – 4384Combined sources
Beta strandi440 – 4467Combined sources
Beta strandi460 – 47011Combined sources
Helixi478 – 4869Combined sources
Turni493 – 4953Combined sources
Helixi496 – 4994Combined sources
Helixi501 – 51313Combined sources
Beta strandi522 – 5298Combined sources
Helixi530 – 5334Combined sources
Helixi535 – 5406Combined sources
Beta strandi547 – 5493Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CRVX-ray2.00A1-551[»]
3CRWX-ray4.0011-551[»]
ProteinModelPortaliQ4JC68.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4JC68.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 228228Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi180 – 1834DEAH box

Domaini

4Fe-4S iron-sulfur-binding is required for protein stability and helicase activity.1 Publication

Sequence similaritiesi

Belongs to the helicase family. RAD3/XPD subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1199.
HOGENOMiHOG000271001.
KOiK10844.
OMAiRTHNEFY.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JC68-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKLRDWQEK LKDKVIEGLR NNFLVALNAP TGSGKTLFSL LVSLEVKPKV
60 70 80 90 100
LFVVRTHNEF YPIYRDLTKI REKRNITFSF LVGKPSSCLY AEKGAESEDI
110 120 130 140 150
PCKYCELKGS IVEVKTDDSP LSLVKKLKKD GLQDKFCPYY SLLNSLYKAD
160 170 180 190 200
VIALTYPYFF IDRYREFIDI DLREYMIVID EAHNLDKVNE LEERSLSEIT
210 220 230 240 250
IQMAIKQSKS EESRRILSKL LNQLREVVLP DEKYIKVENV PKLSKEELEI
260 270 280 290 300
LADDYEDIRK DSLKQGKVNK IHIGSILRFF SLLSIGSFIP FSYSKRLVIK
310 320 330 340 350
NPEISYYLNL LNDNELSIIL MSGTLPPREY MEKVWGIKRN MLYLDVEREI
360 370 380 390 400
QKRVSGSYEC YIGVDVTSKY DMRSDNMWKR YADYLLKIYF QAKANVLVVF
410 420 430 440 450
PSYEIMDRVM SRISLPKYVE SEDSSVEDLY SAISANNKVL IGSVGKGKLA
460 470 480 490 500
EGIELRNNDR SLISDVVIVG IPYPPPDDYL KILAQRVSLK MNRENEEFLF
510 520 530 540 550
KIPALVTIKQ AIGRAIRDVN DKCNVWLLDK RFESLYWKKN LKCLNANKMK

L
Length:551
Mass (Da):64,011
Last modified:August 2, 2005 - v1
Checksum:iBEE17759C4E065DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79611.1.
RefSeqiYP_254904.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79611; AAY79611; Saci_0192.
GeneIDi3474005.
KEGGisai:Saci_0192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79611.1 .
RefSeqi YP_254904.1. NC_007181.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3CRV X-ray 2.00 A 1-551 [» ]
3CRW X-ray 4.00 1 1-551 [» ]
ProteinModelPortali Q4JC68.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 330779.Saci_0192.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY79611 ; AAY79611 ; Saci_0192 .
GeneIDi 3474005.
KEGGi sai:Saci_0192.

Phylogenomic databases

eggNOGi COG1199.
HOGENOMi HOG000271001.
KOi K10844.
OMAi RTHNEFY.

Enzyme and pathway databases

BioCyci SACI330779:GH9J-191-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q4JC68.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view ]
SMARTi SM00491. HELICc2. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
    Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
    J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
  2. "The DNA repair helicases XPD and FancJ have essential iron-sulfur domains."
    Rudolf J., Makrantoni V., Ingledew W.J., Stark M.J., White M.F.
    Mol. Cell 23:801-808(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, MUTAGENESIS OF LYS-35; LYS-84; CYS-88; CYS-102; CYS-105; PHE-136 AND CYS-137.
  3. "XPD helicase structures and activities: insights into the cancer and aging phenotypes from XPD mutations."
    Fan L., Fuss J.O., Cheng Q.J., Arvai A.S., Hammel M., Roberts V.A., Cooper P.K., Tainer J.A.
    Cell 133:789-800(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), IRON-SULFUR CLUSTER.

Entry informationi

Entry nameiXPD_SULAC
AccessioniPrimary (citable) accession number: Q4JC68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: August 2, 2005
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3