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Q4JC68

- XPD_SULAC

UniProt

Q4JC68 - XPD_SULAC

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Protein

ATP-dependent DNA helicase Saci_0192

Gene
Saci_0192
Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATP-dependent 5'-3' DNA helicase involved in nucleotide excision repair (NER) of DNA.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Cofactori

Binds 1 4Fe-4S cluster.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron-sulfur (4Fe-4S)
Metal bindingi102 – 1021Iron-sulfur (4Fe-4S)
Metal bindingi105 – 1051Iron-sulfur (4Fe-4S)
Metal bindingi137 – 1371Iron-sulfur (4Fe-4S)

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 368ATP By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB
  2. 5'-3' DNA helicase activity Source: UniProtKB
  3. ATP binding Source: UniProtKB-KW
  4. ATP-dependent DNA helicase activity Source: InterPro
  5. DNA binding Source: UniProtKB-KW
  6. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA duplex unwinding Source: UniProtKB
  2. DNA repair Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription

Keywords - Ligandi

4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSACI330779:GH9J-191-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Saci_0192 (EC:3.6.4.12)
Gene namesi
Ordered Locus Names:Saci_0192
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001018: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351K → A: Abolishes helicase activity but not iron-sulfur-binding. 1 Publication
Mutagenesisi84 – 841K → H: Impairs iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi88 – 881C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi102 – 1021C → S: Does not affect iron-sulfur-binding nor helicase activity. 1 Publication
Mutagenesisi105 – 1051C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi136 – 1361F → P: Impairs iron-sulfur-binding and helicase activity. 1 Publication
Mutagenesisi137 – 1371C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551ATP-dependent DNA helicase Saci_0192PRO_0000352309Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi330779.Saci_0192.

Structurei

Secondary structure

1
551
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2015
Beta strandi24 – 285
Helixi35 – 4612
Beta strandi48 – 569
Helixi57 – 593
Helixi60 – 678
Beta strandi78 – 803
Helixi84 – 874
Helixi97 – 993
Helixi102 – 1043
Turni106 – 1094
Helixi120 – 13415
Helixi138 – 1458
Helixi146 – 1483
Beta strandi150 – 1556
Helixi157 – 1604
Helixi162 – 1654
Beta strandi175 – 1795
Helixi182 – 19211
Beta strandi194 – 1963
Helixi198 – 20710
Helixi211 – 22414
Helixi245 – 26420
Helixi272 – 28514
Beta strandi289 – 2935
Beta strandi296 – 3005
Helixi305 – 3084
Helixi309 – 3124
Beta strandi317 – 3248
Helixi328 – 3336
Beta strandi341 – 3455
Helixi346 – 3494
Beta strandi357 – 3637
Turni370 – 3723
Helixi375 – 39117
Beta strandi393 – 4019
Helixi403 – 4108
Beta strandi415 – 4195
Helixi426 – 4327
Beta strandi435 – 4384
Beta strandi440 – 4467
Beta strandi460 – 47011
Helixi478 – 4869
Turni493 – 4953
Helixi496 – 4994
Helixi501 – 51313
Beta strandi522 – 5298
Helixi530 – 5334
Helixi535 – 5406
Beta strandi547 – 5493

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CRVX-ray2.00A1-551[»]
3CRWX-ray4.0011-551[»]
ProteinModelPortaliQ4JC68.

Miscellaneous databases

EvolutionaryTraceiQ4JC68.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 228228Helicase ATP-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi180 – 1834DEAH box

Domaini

4Fe-4S iron-sulfur-binding is required for protein stability and helicase activity (1 Publication).

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1199.
HOGENOMiHOG000271001.
KOiK10844.
OMAiRTHNEFY.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JC68-1 [UniParc]FASTAAdd to Basket

« Hide

MLKLRDWQEK LKDKVIEGLR NNFLVALNAP TGSGKTLFSL LVSLEVKPKV    50
LFVVRTHNEF YPIYRDLTKI REKRNITFSF LVGKPSSCLY AEKGAESEDI 100
PCKYCELKGS IVEVKTDDSP LSLVKKLKKD GLQDKFCPYY SLLNSLYKAD 150
VIALTYPYFF IDRYREFIDI DLREYMIVID EAHNLDKVNE LEERSLSEIT 200
IQMAIKQSKS EESRRILSKL LNQLREVVLP DEKYIKVENV PKLSKEELEI 250
LADDYEDIRK DSLKQGKVNK IHIGSILRFF SLLSIGSFIP FSYSKRLVIK 300
NPEISYYLNL LNDNELSIIL MSGTLPPREY MEKVWGIKRN MLYLDVEREI 350
QKRVSGSYEC YIGVDVTSKY DMRSDNMWKR YADYLLKIYF QAKANVLVVF 400
PSYEIMDRVM SRISLPKYVE SEDSSVEDLY SAISANNKVL IGSVGKGKLA 450
EGIELRNNDR SLISDVVIVG IPYPPPDDYL KILAQRVSLK MNRENEEFLF 500
KIPALVTIKQ AIGRAIRDVN DKCNVWLLDK RFESLYWKKN LKCLNANKMK 550
L 551
Length:551
Mass (Da):64,011
Last modified:August 2, 2005 - v1
Checksum:iBEE17759C4E065DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000077 Genomic DNA. Translation: AAY79611.1.
RefSeqiYP_254904.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79611; AAY79611; Saci_0192.
GeneIDi3474005.
KEGGisai:Saci_0192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000077 Genomic DNA. Translation: AAY79611.1 .
RefSeqi YP_254904.1. NC_007181.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3CRV X-ray 2.00 A 1-551 [» ]
3CRW X-ray 4.00 1 1-551 [» ]
ProteinModelPortali Q4JC68.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 330779.Saci_0192.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY79611 ; AAY79611 ; Saci_0192 .
GeneIDi 3474005.
KEGGi sai:Saci_0192.

Phylogenomic databases

eggNOGi COG1199.
HOGENOMi HOG000271001.
KOi K10844.
OMAi RTHNEFY.

Enzyme and pathway databases

BioCyci SACI330779:GH9J-191-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q4JC68.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view ]
SMARTi SM00491. HELICc2. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
    Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
    J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
  2. "The DNA repair helicases XPD and FancJ have essential iron-sulfur domains."
    Rudolf J., Makrantoni V., Ingledew W.J., Stark M.J., White M.F.
    Mol. Cell 23:801-808(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, MUTAGENESIS OF LYS-35; LYS-84; CYS-88; CYS-102; CYS-105; PHE-136 AND CYS-137.
  3. "XPD helicase structures and activities: insights into the cancer and aging phenotypes from XPD mutations."
    Fan L., Fuss J.O., Cheng Q.J., Arvai A.S., Hammel M., Roberts V.A., Cooper P.K., Tainer J.A.
    Cell 133:789-800(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), IRON-SULFUR CLUSTER.

Entry informationi

Entry nameiXPD_SULAC
AccessioniPrimary (citable) accession number: Q4JC68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: August 2, 2005
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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