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Q4JC68 (XPD_SULAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent DNA helicase Saci_0192

EC=3.6.4.12
Gene names
Ordered Locus Names:Saci_0192
OrganismSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]
Taxonomic identifier330779 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATP-dependent 5'-3' DNA helicase involved in nucleotide excision repair (NER) of DNA. Ref.2

Catalytic activity

ATP + H2O = ADP + phosphate. Ref.2

Cofactor

Binds 1 4Fe-4S cluster. Ref.2

Domain

4Fe-4S iron-sulfur-binding is required for protein stability and helicase activity (Ref.3).

Sequence similarities

Belongs to the helicase family. RAD3/XPD subfamily.

Contains 1 helicase ATP-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551ATP-dependent DNA helicase Saci_0192
PRO_0000352309

Regions

Domain1 – 228228Helicase ATP-binding
Nucleotide binding29 – 368ATP By similarity
Motif180 – 1834DEAH box

Sites

Metal binding881Iron-sulfur (4Fe-4S)
Metal binding1021Iron-sulfur (4Fe-4S)
Metal binding1051Iron-sulfur (4Fe-4S)
Metal binding1371Iron-sulfur (4Fe-4S)

Experimental info

Mutagenesis351K → A: Abolishes helicase activity but not iron-sulfur-binding. Ref.2
Mutagenesis841K → H: Impairs iron-sulfur-binding and helicase activity. Ref.2
Mutagenesis881C → S: Abolishes iron-sulfur-binding and helicase activity. Ref.2
Mutagenesis1021C → S: Does not affect iron-sulfur-binding nor helicase activity. Ref.2
Mutagenesis1051C → S: Abolishes iron-sulfur-binding and helicase activity. Ref.2
Mutagenesis1361F → P: Impairs iron-sulfur-binding and helicase activity. Ref.2
Mutagenesis1371C → S: Abolishes iron-sulfur-binding and helicase activity. Ref.2

Secondary structure

.............................................................................................. 551
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q4JC68 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: BEE17759C4E065DA

FASTA55164,011
        10         20         30         40         50         60 
MLKLRDWQEK LKDKVIEGLR NNFLVALNAP TGSGKTLFSL LVSLEVKPKV LFVVRTHNEF 

        70         80         90        100        110        120 
YPIYRDLTKI REKRNITFSF LVGKPSSCLY AEKGAESEDI PCKYCELKGS IVEVKTDDSP 

       130        140        150        160        170        180 
LSLVKKLKKD GLQDKFCPYY SLLNSLYKAD VIALTYPYFF IDRYREFIDI DLREYMIVID 

       190        200        210        220        230        240 
EAHNLDKVNE LEERSLSEIT IQMAIKQSKS EESRRILSKL LNQLREVVLP DEKYIKVENV 

       250        260        270        280        290        300 
PKLSKEELEI LADDYEDIRK DSLKQGKVNK IHIGSILRFF SLLSIGSFIP FSYSKRLVIK 

       310        320        330        340        350        360 
NPEISYYLNL LNDNELSIIL MSGTLPPREY MEKVWGIKRN MLYLDVEREI QKRVSGSYEC 

       370        380        390        400        410        420 
YIGVDVTSKY DMRSDNMWKR YADYLLKIYF QAKANVLVVF PSYEIMDRVM SRISLPKYVE 

       430        440        450        460        470        480 
SEDSSVEDLY SAISANNKVL IGSVGKGKLA EGIELRNNDR SLISDVVIVG IPYPPPDDYL 

       490        500        510        520        530        540 
KILAQRVSLK MNRENEEFLF KIPALVTIKQ AIGRAIRDVN DKCNVWLLDK RFESLYWKKN 

       550 
LKCLNANKMK L 

« Hide

References

« Hide 'large scale' references
[1]"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[2]"The DNA repair helicases XPD and FancJ have essential iron-sulfur domains."
Rudolf J., Makrantoni V., Ingledew W.J., Stark M.J., White M.F.
Mol. Cell 23:801-808(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, MUTAGENESIS OF LYS-35; LYS-84; CYS-88; CYS-102; CYS-105; PHE-136 AND CYS-137.
[3]"XPD helicase structures and activities: insights into the cancer and aging phenotypes from XPD mutations."
Fan L., Fuss J.O., Cheng Q.J., Arvai A.S., Hammel M., Roberts V.A., Cooper P.K., Tainer J.A.
Cell 133:789-800(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), IRON-SULFUR CLUSTER.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000077 Genomic DNA. Translation: AAY79611.1.
RefSeqYP_254904.1. NC_007181.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CRVX-ray2.00A3-551[»]
3CRWX-ray4.0013-551[»]
ProteinModelPortalQ4JC68.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING330779.Saci_0192.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY79611; AAY79611; Saci_0192.
GeneID3474005.
KEGGsai:Saci_0192.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1199.
HOGENOMHOG000271001.
KOK10844.
OMARENYLTI.
ProtClustDBCLSK802665.

Enzyme and pathway databases

BioCycSACI330779:GH9J-191-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 3 hits.
InterProIPR006555. ATP-dep_Helicase_C.
IPR027076. CHL1.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11472:SF5. PTHR11472:SF5. 1 hit.
PfamPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTSM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
PROSITEPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ4JC68.

Entry information

Entry nameXPD_SULAC
AccessionPrimary (citable) accession number: Q4JC68
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references