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Protein

ATP-dependent DNA helicase Saci_0192

Gene

Saci_0192

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent 5'-3' DNA helicase involved in nucleotide excision repair (NER) of DNA.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi88Iron-sulfur (4Fe-4S)1
Metal bindingi102Iron-sulfur (4Fe-4S)1
Metal bindingi105Iron-sulfur (4Fe-4S)1
Metal bindingi137Iron-sulfur (4Fe-4S)1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 36ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB
  • 5'-3' DNA helicase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA duplex unwinding Source: UniProtKB
  • DNA repair Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription

Keywords - Ligandi

4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSACI330779:GH9J-190-MONOMER.
BRENDAi3.6.4.12. 6160.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Saci_0192 (EC:3.6.4.12)
Gene namesi
Ordered Locus Names:Saci_0192
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35K → A: Abolishes helicase activity but not iron-sulfur-binding. 1 Publication1
Mutagenesisi84K → H: Impairs iron-sulfur-binding and helicase activity. 1 Publication1
Mutagenesisi88C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication1
Mutagenesisi102C → S: Does not affect iron-sulfur-binding nor helicase activity. 1 Publication1
Mutagenesisi105C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication1
Mutagenesisi136F → P: Impairs iron-sulfur-binding and helicase activity. 1 Publication1
Mutagenesisi137C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003523091 – 551ATP-dependent DNA helicase Saci_0192Add BLAST551

Interactioni

Protein-protein interaction databases

STRINGi330779.Saci_0192.

Structurei

Secondary structure

1551
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 20Combined sources15
Beta strandi24 – 28Combined sources5
Beta strandi31 – 34Combined sources4
Helixi35 – 46Combined sources12
Beta strandi48 – 56Combined sources9
Helixi57 – 59Combined sources3
Helixi60 – 67Combined sources8
Beta strandi78 – 80Combined sources3
Helixi84 – 87Combined sources4
Helixi97 – 99Combined sources3
Helixi102 – 104Combined sources3
Turni106 – 109Combined sources4
Helixi120 – 134Combined sources15
Helixi138 – 145Combined sources8
Helixi146 – 148Combined sources3
Beta strandi150 – 155Combined sources6
Helixi157 – 160Combined sources4
Helixi162 – 165Combined sources4
Helixi172 – 174Combined sources3
Beta strandi175 – 179Combined sources5
Helixi182 – 192Combined sources11
Beta strandi194 – 196Combined sources3
Helixi198 – 207Combined sources10
Helixi211 – 224Combined sources14
Helixi245 – 264Combined sources20
Helixi272 – 285Combined sources14
Beta strandi289 – 293Combined sources5
Beta strandi296 – 300Combined sources5
Helixi305 – 308Combined sources4
Helixi309 – 312Combined sources4
Beta strandi317 – 324Combined sources8
Helixi328 – 333Combined sources6
Beta strandi341 – 345Combined sources5
Helixi346 – 349Combined sources4
Beta strandi357 – 363Combined sources7
Turni370 – 372Combined sources3
Helixi375 – 391Combined sources17
Beta strandi393 – 401Combined sources9
Helixi403 – 410Combined sources8
Beta strandi415 – 419Combined sources5
Helixi426 – 432Combined sources7
Beta strandi435 – 438Combined sources4
Beta strandi440 – 446Combined sources7
Beta strandi460 – 470Combined sources11
Helixi478 – 486Combined sources9
Turni493 – 495Combined sources3
Helixi496 – 499Combined sources4
Helixi501 – 513Combined sources13
Beta strandi522 – 529Combined sources8
Helixi530 – 533Combined sources4
Helixi535 – 540Combined sources6
Beta strandi547 – 549Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CRVX-ray2.00A1-551[»]
3CRWX-ray4.0011-551[»]
5H8CX-ray2.29A3-551[»]
ProteinModelPortaliQ4JC68.
SMRiQ4JC68.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4JC68.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 228Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST228

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi180 – 183DEAH box4

Domaini

4Fe-4S iron-sulfur-binding is required for protein stability and helicase activity.1 Publication

Sequence similaritiesi

Belongs to the helicase family. RAD3/XPD subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG00770. Archaea.
COG1199. LUCA.
HOGENOMiHOG000271001.
KOiK10844.
OMAiRENYLTI.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JC68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLRDWQEK LKDKVIEGLR NNFLVALNAP TGSGKTLFSL LVSLEVKPKV
60 70 80 90 100
LFVVRTHNEF YPIYRDLTKI REKRNITFSF LVGKPSSCLY AEKGAESEDI
110 120 130 140 150
PCKYCELKGS IVEVKTDDSP LSLVKKLKKD GLQDKFCPYY SLLNSLYKAD
160 170 180 190 200
VIALTYPYFF IDRYREFIDI DLREYMIVID EAHNLDKVNE LEERSLSEIT
210 220 230 240 250
IQMAIKQSKS EESRRILSKL LNQLREVVLP DEKYIKVENV PKLSKEELEI
260 270 280 290 300
LADDYEDIRK DSLKQGKVNK IHIGSILRFF SLLSIGSFIP FSYSKRLVIK
310 320 330 340 350
NPEISYYLNL LNDNELSIIL MSGTLPPREY MEKVWGIKRN MLYLDVEREI
360 370 380 390 400
QKRVSGSYEC YIGVDVTSKY DMRSDNMWKR YADYLLKIYF QAKANVLVVF
410 420 430 440 450
PSYEIMDRVM SRISLPKYVE SEDSSVEDLY SAISANNKVL IGSVGKGKLA
460 470 480 490 500
EGIELRNNDR SLISDVVIVG IPYPPPDDYL KILAQRVSLK MNRENEEFLF
510 520 530 540 550
KIPALVTIKQ AIGRAIRDVN DKCNVWLLDK RFESLYWKKN LKCLNANKMK

L
Length:551
Mass (Da):64,011
Last modified:August 2, 2005 - v1
Checksum:iBEE17759C4E065DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79611.1.
RefSeqiWP_011277112.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79611; AAY79611; Saci_0192.
GeneIDi3474005.
KEGGisai:Saci_0192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79611.1.
RefSeqiWP_011277112.1. NC_007181.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CRVX-ray2.00A1-551[»]
3CRWX-ray4.0011-551[»]
5H8CX-ray2.29A3-551[»]
ProteinModelPortaliQ4JC68.
SMRiQ4JC68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_0192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY79611; AAY79611; Saci_0192.
GeneIDi3474005.
KEGGisai:Saci_0192.

Phylogenomic databases

eggNOGiarCOG00770. Archaea.
COG1199. LUCA.
HOGENOMiHOG000271001.
KOiK10844.
OMAiRENYLTI.

Enzyme and pathway databases

BioCyciSACI330779:GH9J-190-MONOMER.
BRENDAi3.6.4.12. 6160.

Miscellaneous databases

EvolutionaryTraceiQ4JC68.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXPD_SULAC
AccessioniPrimary (citable) accession number: Q4JC68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.