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Q4JC68

- XPD_SULAC

UniProt

Q4JC68 - XPD_SULAC

Protein

ATP-dependent DNA helicase Saci_0192

Gene

Saci_0192

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    ATP-dependent 5'-3' DNA helicase involved in nucleotide excision repair (NER) of DNA.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.1 Publication

    Cofactori

    Binds 1 4Fe-4S cluster.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi88 – 881Iron-sulfur (4Fe-4S)
    Metal bindingi102 – 1021Iron-sulfur (4Fe-4S)
    Metal bindingi105 – 1051Iron-sulfur (4Fe-4S)
    Metal bindingi137 – 1371Iron-sulfur (4Fe-4S)

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 368ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB
    2. 5'-3' DNA helicase activity Source: UniProtKB
    3. ATP binding Source: UniProtKB-KW
    4. ATP-dependent DNA helicase activity Source: InterPro
    5. DNA binding Source: UniProtKB-KW
    6. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA duplex unwinding Source: UniProtKB
    2. DNA repair Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair, Transcription

    Keywords - Ligandi

    4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSACI330779:GH9J-191-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent DNA helicase Saci_0192 (EC:3.6.4.12)
    Gene namesi
    Ordered Locus Names:Saci_0192
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001018: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi35 – 351K → A: Abolishes helicase activity but not iron-sulfur-binding. 1 Publication
    Mutagenesisi84 – 841K → H: Impairs iron-sulfur-binding and helicase activity. 1 Publication
    Mutagenesisi88 – 881C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication
    Mutagenesisi102 – 1021C → S: Does not affect iron-sulfur-binding nor helicase activity. 1 Publication
    Mutagenesisi105 – 1051C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication
    Mutagenesisi136 – 1361F → P: Impairs iron-sulfur-binding and helicase activity. 1 Publication
    Mutagenesisi137 – 1371C → S: Abolishes iron-sulfur-binding and helicase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551ATP-dependent DNA helicase Saci_0192PRO_0000352309Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi330779.Saci_0192.

    Structurei

    Secondary structure

    1
    551
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 2015
    Beta strandi24 – 285
    Helixi35 – 4612
    Beta strandi48 – 569
    Helixi57 – 593
    Helixi60 – 678
    Beta strandi78 – 803
    Helixi84 – 874
    Helixi97 – 993
    Helixi102 – 1043
    Turni106 – 1094
    Helixi120 – 13415
    Helixi138 – 1458
    Helixi146 – 1483
    Beta strandi150 – 1556
    Helixi157 – 1604
    Helixi162 – 1654
    Beta strandi175 – 1795
    Helixi182 – 19211
    Beta strandi194 – 1963
    Helixi198 – 20710
    Helixi211 – 22414
    Helixi245 – 26420
    Helixi272 – 28514
    Beta strandi289 – 2935
    Beta strandi296 – 3005
    Helixi305 – 3084
    Helixi309 – 3124
    Beta strandi317 – 3248
    Helixi328 – 3336
    Beta strandi341 – 3455
    Helixi346 – 3494
    Beta strandi357 – 3637
    Turni370 – 3723
    Helixi375 – 39117
    Beta strandi393 – 4019
    Helixi403 – 4108
    Beta strandi415 – 4195
    Helixi426 – 4327
    Beta strandi435 – 4384
    Beta strandi440 – 4467
    Beta strandi460 – 47011
    Helixi478 – 4869
    Turni493 – 4953
    Helixi496 – 4994
    Helixi501 – 51313
    Beta strandi522 – 5298
    Helixi530 – 5334
    Helixi535 – 5406
    Beta strandi547 – 5493

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3CRVX-ray2.00A1-551[»]
    3CRWX-ray4.0011-551[»]
    ProteinModelPortaliQ4JC68.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ4JC68.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 228228Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi180 – 1834DEAH box

    Domaini

    4Fe-4S iron-sulfur-binding is required for protein stability and helicase activity.1 Publication

    Sequence similaritiesi

    Belongs to the helicase family. RAD3/XPD subfamily.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1199.
    HOGENOMiHOG000271001.
    KOiK10844.
    OMAiRTHNEFY.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR006555. ATP-dep_Helicase_C.
    IPR010614. DEAD_2.
    IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF06733. DEAD_2. 1 hit.
    PF13307. Helicase_C_2. 1 hit.
    [Graphical view]
    SMARTiSM00491. HELICc2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4JC68-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKLRDWQEK LKDKVIEGLR NNFLVALNAP TGSGKTLFSL LVSLEVKPKV    50
    LFVVRTHNEF YPIYRDLTKI REKRNITFSF LVGKPSSCLY AEKGAESEDI 100
    PCKYCELKGS IVEVKTDDSP LSLVKKLKKD GLQDKFCPYY SLLNSLYKAD 150
    VIALTYPYFF IDRYREFIDI DLREYMIVID EAHNLDKVNE LEERSLSEIT 200
    IQMAIKQSKS EESRRILSKL LNQLREVVLP DEKYIKVENV PKLSKEELEI 250
    LADDYEDIRK DSLKQGKVNK IHIGSILRFF SLLSIGSFIP FSYSKRLVIK 300
    NPEISYYLNL LNDNELSIIL MSGTLPPREY MEKVWGIKRN MLYLDVEREI 350
    QKRVSGSYEC YIGVDVTSKY DMRSDNMWKR YADYLLKIYF QAKANVLVVF 400
    PSYEIMDRVM SRISLPKYVE SEDSSVEDLY SAISANNKVL IGSVGKGKLA 450
    EGIELRNNDR SLISDVVIVG IPYPPPDDYL KILAQRVSLK MNRENEEFLF 500
    KIPALVTIKQ AIGRAIRDVN DKCNVWLLDK RFESLYWKKN LKCLNANKMK 550
    L 551
    Length:551
    Mass (Da):64,011
    Last modified:August 2, 2005 - v1
    Checksum:iBEE17759C4E065DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79611.1.
    RefSeqiYP_254904.1. NC_007181.1.

    Genome annotation databases

    EnsemblBacteriaiAAY79611; AAY79611; Saci_0192.
    GeneIDi3474005.
    KEGGisai:Saci_0192.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79611.1 .
    RefSeqi YP_254904.1. NC_007181.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3CRV X-ray 2.00 A 1-551 [» ]
    3CRW X-ray 4.00 1 1-551 [» ]
    ProteinModelPortali Q4JC68.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 330779.Saci_0192.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY79611 ; AAY79611 ; Saci_0192 .
    GeneIDi 3474005.
    KEGGi sai:Saci_0192.

    Phylogenomic databases

    eggNOGi COG1199.
    HOGENOMi HOG000271001.
    KOi K10844.
    OMAi RTHNEFY.

    Enzyme and pathway databases

    BioCyci SACI330779:GH9J-191-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q4JC68.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR006555. ATP-dep_Helicase_C.
    IPR010614. DEAD_2.
    IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF06733. DEAD_2. 1 hit.
    PF13307. Helicase_C_2. 1 hit.
    [Graphical view ]
    SMARTi SM00491. HELICc2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    PROSITEi PS51193. HELICASE_ATP_BIND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
      Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
      J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
    2. "The DNA repair helicases XPD and FancJ have essential iron-sulfur domains."
      Rudolf J., Makrantoni V., Ingledew W.J., Stark M.J., White M.F.
      Mol. Cell 23:801-808(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, MUTAGENESIS OF LYS-35; LYS-84; CYS-88; CYS-102; CYS-105; PHE-136 AND CYS-137.
    3. "XPD helicase structures and activities: insights into the cancer and aging phenotypes from XPD mutations."
      Fan L., Fuss J.O., Cheng Q.J., Arvai A.S., Hammel M., Roberts V.A., Cooper P.K., Tainer J.A.
      Cell 133:789-800(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), IRON-SULFUR CLUSTER.

    Entry informationi

    Entry nameiXPD_SULAC
    AccessioniPrimary (citable) accession number: Q4JC68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3