Q4JBP0 (SYL1_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase 1
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase 1
Short name=LeuRS 1

Gene names

Name:	leuS1
Ordered Locus Names:	Saci_0373

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus acidocaldarius

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 942	942	Leucine--tRNA ligase 1 HAMAP-Rule MF_00049		PRO_0000152142
Regions
	Motif	39 – 49	11	"HIGH" region HAMAP-Rule MF_00049
	Motif	624 – 628	5	"KMSKS" region HAMAP-Rule MF_00049
Sites
	Binding site	627	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JBP0 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: C62D780A5A6C432F	FASTA	942	108,215
	10 20 30 40 50 60 MDFLNEIASK WQKVWDSERV YEADVDRTKE KKFITVAFPY TNSPLHIGHG RTYITADIYA 70 80 90 100 110 120 RYLRMKGYNV LFPFAFQFTG TPILSIADAV KRGDEEIIST FTNVYQIPIG VISKFSDPSY 130 140 150 160 170 180 LSAYFKEDMR NTALTLGLSI DRRREFTTID PAFERFVQWQ YKRLQEIGYI KKEKAPVAYC 190 200 210 220 230 240 PVDEFPVGMH DTRGDIEPEI IDLDVIYFQG EKLLFLTATS RPETIFGAVA ILINPDSDYS 250 260 270 280 290 300 IVVDNKNGKR LVMSTEAFKK LSFQMSLTEE ERKKGGELIG LNVTNPVTLK KLTVLPSKYV 310 320 330 340 350 360 ESKQGTGVVM AVPAHEPLHY LALSELKESF EIVPVIKSED YGDFPAMEVL ETAQTTSAQE 370 380 390 400 410 420 LKDYIDTLYR IEFHKGSIRD EVVDLVPDYM KQFVGERIAG KSVREARSSV VELLRNLGVH 430 440 450 460 470 480 GNIYEIINGP VYCRCGAEVV VKVFDDQWFI DYSNSTWKSS VLKSLDKIEI LPQDAKREIS 490 500 510 520 530 540 KIIFNMKPRP FTRSRGLGVR LPWDDRQIID SLSDSTIYTV FYIVANKVKS YPTSILNERF 550 560 570 580 590 600 WDYVVLGRGD SSQLSRELGI PKEQLEELRM EVEYWYPVDS RHSGRDLVQN HIPYYLYHHV 610 620 630 640 650 660 GVLGEDKVPK RIVLNGFIRV GGKKMSKSFG NVYPLNKAIR EYGVDTVRLA LTSTSSLSDD 670 680 690 700 710 720 IEFSPNIAKS IGEQLKHIHD FIENLIKLQS VNEIRKVDLW ISSLISEYID LIDNCLSNLD 730 740 750 760 770 780 LRTAYKTIYY DIYEDLKDYL ELGNGKINSD IIKNVISVWI RLMAPFTPHL AEELWHKLDN 790 800 810 820 830 840 SLVVRQRFPS KGELQYDKRA LLEIEYLRYT IDLINSMKSK MSKEPETVII YVNEDNTQRD 850 860 870 880 890 900 LIRKAIESLK ERKSLPDFEK EVGDREMARL AYEIAGDLPD KIKNLAEIGI NESEILTSNA 910 920 930 940 QFLLNKLDVK EIYIYNSKDP STPDIKGKKS IALPYKPGII LT

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY79789.1.
RefSeq	YP_255082.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4JBP0.
ModBase	Search...
Protein-protein interaction databases
STRING	330779.Saci_0373.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY79789; AAY79789; Saci_0373.
GeneID	3473317.
KEGG	sai:Saci_0373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000216620.
KO	K01869.
OMA	NSPLHIG.
ProtClustDB	PRK12300.
Enzyme and pathway databases
BioCyc	SACI330779:GH9J-448-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_A. Leu_tRNA_synth_A.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR020791. Leu-tRNA-lgase_arc. IPR004493. Leu-tRNA-synth_Ia_arc/euk. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00395. leuS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL1_SULAC

Accession

Primary (citable) accession number: Q4JBP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 2, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program