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Q4JB80 (DPO4_SULAC) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase IV

Short name=Pol IV
EC=2.7.7.7
Gene names
Name:dbh
Ordered Locus Names:Saci_0554
OrganismSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]
Taxonomic identifier330779 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis By similarity. HAMAP-Rule MF_01113

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_01113

Cofactor

Binds 2 magnesium ions per subunit By similarity. HAMAP-Rule MF_01113

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01113

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01113.

Sequence similarities

Belongs to the DNA polymerase type-Y family.

Contains 1 umuC domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   Cellular componentCytoplasm
   LigandDNA-binding
Magnesium
Metal-binding
   Molecular functionDNA-directed DNA polymerase
Mutator protein
Nucleotidyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

damaged DNA binding

Inferred from electronic annotation. Source: InterPro

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354DNA polymerase IV HAMAP-Rule MF_01113
PRO_0000173974

Regions

Domain3 – 188186UmuC

Sites

Active site1061 By similarity
Metal binding71Magnesium By similarity
Metal binding1051Magnesium By similarity
Site121Substrate discrimination By similarity

Secondary structure

.................................................................. 354
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q4JB80 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 0EDDAEB0F30EAD35

FASTA35439,986
        10         20         30         40         50         60 
MIVIFVDFDY FFAQVEEVLN PQYKGKPLVV CVYSGRTKTS GAVATANYEA RKLGVKAGMP 

        70         80         90        100        110        120 
IIKAMQIAPS AIYVPMRKPI YEAFSNRIMN LLNKHADKIE VASIDEAYLD VTNKVEGNFE 

       130        140        150        160        170        180 
NGIELARKIK QEILEKEKIT VTVGVAPNKI LAKIIADKSK PNGLGVIRPT EVQDFLNELD 

       190        200        210        220        230        240 
IDEIPGIGSV LARRLNELGI QKLRDILSKN YNELEKITGK AKALYLLKLA QNKYSEPVEN 

       250        260        270        280        290        300 
KSKIPHGRYL TLPYNTRDVK VILPYLKKAI NEAYNKVNGI PMRITVIAIM EDLDILSKGK 

       310        320        330        340        350 
KFKHGISIDN AYKVAEDLLR ELLVRDKRRN VRRIGVKLDN IIINKTNLSD FFDI 

« Hide

References

[1]"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000077 Genomic DNA. Translation: AAY79949.1.
RefSeqYP_255242.1. NC_007181.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3BQ0X-ray2.60A1-354[»]
3BQ1X-ray2.70A1-354[»]
3BQ2X-ray2.70A1-354[»]
4F4WX-ray1.90A/B1-231[»]
4F4XX-ray2.05A1-231[»]
4F4YX-ray2.34A/B1-354[»]
4F4ZX-ray2.30A/B248-354[»]
4F50X-ray2.22A1-248[»]
4HYKX-ray2.80A1-354[»]
4NLGX-ray2.40A1-354[»]
ProteinModelPortalQ4JB80.
SMRQ4JB80. Positions 1-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING330779.Saci_0554.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY79949; AAY79949; Saci_0554.
GeneID3474616.
KEGGsai:Saci_0554.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0389.
HOGENOMHOG000082707.
KOK04479.
OMADFDYFFA.
ProtClustDBPRK01216.

Enzyme and pathway databases

BioCycSACI330779:GH9J-546-MONOMER.

Family and domain databases

Gene3D3.30.1490.100. 1 hit.
HAMAPMF_01113. DNApol_IV.
InterProIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
[Graphical view]
PfamPF00817. IMS. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMSSF100879. SSF100879. 1 hit.
PROSITEPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ4JB80.

Entry information

Entry nameDPO4_SULAC
AccessionPrimary (citable) accession number: Q4JB80
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: August 2, 2005
Last modified: March 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references