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Q4JB80

- DPO4_SULAC

UniProt

Q4JB80 - DPO4_SULAC

Protein

DNA polymerase IV

Gene

dbh

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.UniRule annotation

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

    Cofactori

    Binds 2 magnesium ions per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71MagnesiumUniRule annotation
    Sitei12 – 121Substrate discriminationUniRule annotation
    Metal bindingi105 – 1051MagnesiumUniRule annotation
    Active sitei106 – 1061UniRule annotation

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. DNA-directed DNA polymerase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replication

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSACI330779:GH9J-546-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
    Short name:
    Pol IVUniRule annotation
    Gene namesi
    Name:dbhUniRule annotation
    Ordered Locus Names:Saci_0554
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001018: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354DNA polymerase IVPRO_0000173974Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi330779.Saci_0554.

    Structurei

    Secondary structure

    1
    354
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Helixi11 – 199
    Helixi21 – 233
    Beta strandi28 – 336
    Beta strandi35 – 395
    Beta strandi41 – 466
    Helixi48 – 514
    Turni52 – 543
    Helixi61 – 677
    Beta strandi71 – 755
    Helixi78 – 9316
    Beta strandi97 – 1037
    Beta strandi106 – 1105
    Turni112 – 1176
    Helixi119 – 13719
    Beta strandi141 – 1488
    Helixi149 – 15911
    Beta strandi164 – 1663
    Helixi169 – 1713
    Helixi172 – 1787
    Helixi181 – 1833
    Helixi189 – 1979
    Helixi203 – 2086
    Helixi211 – 2188
    Helixi220 – 23011
    Beta strandi240 – 2423
    Beta strandi248 – 2569
    Helixi259 – 27618
    Beta strandi278 – 2803
    Beta strandi282 – 2909
    Beta strandi295 – 3017
    Helixi308 – 32518
    Beta strandi327 – 3293
    Beta strandi331 – 34010

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BQ0X-ray2.60A1-354[»]
    3BQ1X-ray2.70A1-354[»]
    3BQ2X-ray2.70A1-354[»]
    4F4WX-ray1.90A/B1-231[»]
    4F4XX-ray2.05A1-231[»]
    4F4YX-ray2.34A/B1-127[»]
    A/B213-227[»]
    A/B277-354[»]
    4F4ZX-ray2.30A/B248-354[»]
    4F50X-ray2.22A1-246[»]
    4HYKX-ray2.80A1-354[»]
    4NLGX-ray2.40A1-354[»]
    ProteinModelPortaliQ4JB80.
    SMRiQ4JB80. Positions 1-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ4JB80.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 188186UmuCUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-Y family.UniRule annotation
    Contains 1 umuC domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0389.
    HOGENOMiHOG000082707.
    KOiK04479.
    OMAiPHGRYLT.

    Family and domain databases

    Gene3Di3.30.1490.100. 1 hit.
    HAMAPiMF_01113. DNApol_IV.
    InterProiIPR017961. DNA_pol_Y-fam_little_finger.
    IPR001126. DNA_repair_prot_UmuC-like.
    IPR017963. DNA_repair_prot_UmuC-like_N.
    IPR022880. DNApol_IV.
    IPR024728. PolY_HhH_motif.
    [Graphical view]
    PfamiPF00817. IMS. 1 hit.
    PF11798. IMS_HHH. 1 hit.
    [Graphical view]
    SUPFAMiSSF100879. SSF100879. 1 hit.
    PROSITEiPS50173. UMUC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4JB80-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIVIFVDFDY FFAQVEEVLN PQYKGKPLVV CVYSGRTKTS GAVATANYEA    50
    RKLGVKAGMP IIKAMQIAPS AIYVPMRKPI YEAFSNRIMN LLNKHADKIE 100
    VASIDEAYLD VTNKVEGNFE NGIELARKIK QEILEKEKIT VTVGVAPNKI 150
    LAKIIADKSK PNGLGVIRPT EVQDFLNELD IDEIPGIGSV LARRLNELGI 200
    QKLRDILSKN YNELEKITGK AKALYLLKLA QNKYSEPVEN KSKIPHGRYL 250
    TLPYNTRDVK VILPYLKKAI NEAYNKVNGI PMRITVIAIM EDLDILSKGK 300
    KFKHGISIDN AYKVAEDLLR ELLVRDKRRN VRRIGVKLDN IIINKTNLSD 350
    FFDI 354
    Length:354
    Mass (Da):39,986
    Last modified:August 2, 2005 - v1
    Checksum:i0EDDAEB0F30EAD35
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79949.1.
    RefSeqiYP_255242.1. NC_007181.1.

    Genome annotation databases

    EnsemblBacteriaiAAY79949; AAY79949; Saci_0554.
    GeneIDi3474616.
    KEGGisai:Saci_0554.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79949.1 .
    RefSeqi YP_255242.1. NC_007181.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BQ0 X-ray 2.60 A 1-354 [» ]
    3BQ1 X-ray 2.70 A 1-354 [» ]
    3BQ2 X-ray 2.70 A 1-354 [» ]
    4F4W X-ray 1.90 A/B 1-231 [» ]
    4F4X X-ray 2.05 A 1-231 [» ]
    4F4Y X-ray 2.34 A/B 1-127 [» ]
    A/B 213-227 [» ]
    A/B 277-354 [» ]
    4F4Z X-ray 2.30 A/B 248-354 [» ]
    4F50 X-ray 2.22 A 1-246 [» ]
    4HYK X-ray 2.80 A 1-354 [» ]
    4NLG X-ray 2.40 A 1-354 [» ]
    ProteinModelPortali Q4JB80.
    SMRi Q4JB80. Positions 1-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 330779.Saci_0554.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY79949 ; AAY79949 ; Saci_0554 .
    GeneIDi 3474616.
    KEGGi sai:Saci_0554.

    Phylogenomic databases

    eggNOGi COG0389.
    HOGENOMi HOG000082707.
    KOi K04479.
    OMAi PHGRYLT.

    Enzyme and pathway databases

    BioCyci SACI330779:GH9J-546-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q4JB80.

    Family and domain databases

    Gene3Di 3.30.1490.100. 1 hit.
    HAMAPi MF_01113. DNApol_IV.
    InterProi IPR017961. DNA_pol_Y-fam_little_finger.
    IPR001126. DNA_repair_prot_UmuC-like.
    IPR017963. DNA_repair_prot_UmuC-like_N.
    IPR022880. DNApol_IV.
    IPR024728. PolY_HhH_motif.
    [Graphical view ]
    Pfami PF00817. IMS. 1 hit.
    PF11798. IMS_HHH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF100879. SSF100879. 1 hit.
    PROSITEi PS50173. UMUC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
      Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
      J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

    Entry informationi

    Entry nameiDPO4_SULAC
    AccessioniPrimary (citable) accession number: Q4JB80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3