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Protein

DNA polymerase IV

Gene

dbh

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Sitei12Substrate discriminationUniRule annotation1
Metal bindingi105MagnesiumUniRule annotation1
Active sitei106UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.7.7. 6160.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dbhUniRule annotation
Ordered Locus Names:Saci_0554
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739741 – 354DNA polymerase IVAdd BLAST354

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi330779.Saci_0554.

Structurei

Secondary structure

1354
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi11 – 19Combined sources9
Helixi21 – 23Combined sources3
Beta strandi28 – 33Combined sources6
Beta strandi35 – 39Combined sources5
Beta strandi41 – 46Combined sources6
Helixi48 – 51Combined sources4
Turni52 – 54Combined sources3
Helixi61 – 67Combined sources7
Beta strandi71 – 75Combined sources5
Helixi78 – 93Combined sources16
Beta strandi97 – 103Combined sources7
Beta strandi106 – 110Combined sources5
Turni112 – 117Combined sources6
Helixi119 – 137Combined sources19
Beta strandi141 – 148Combined sources8
Helixi149 – 159Combined sources11
Beta strandi164 – 166Combined sources3
Helixi169 – 171Combined sources3
Helixi172 – 178Combined sources7
Helixi181 – 183Combined sources3
Helixi189 – 197Combined sources9
Helixi203 – 208Combined sources6
Helixi211 – 218Combined sources8
Helixi220 – 230Combined sources11
Beta strandi240 – 242Combined sources3
Beta strandi248 – 256Combined sources9
Helixi259 – 276Combined sources18
Beta strandi278 – 280Combined sources3
Beta strandi282 – 290Combined sources9
Beta strandi295 – 301Combined sources7
Helixi308 – 325Combined sources18
Beta strandi326 – 328Combined sources3
Beta strandi331 – 340Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BQ0X-ray2.60A1-354[»]
3BQ1X-ray2.70A1-354[»]
3BQ2X-ray2.70A1-354[»]
4F4WX-ray1.90A/B1-231[»]
4F4XX-ray2.05A1-231[»]
4F4YX-ray2.34A/B1-354[»]
4F4ZX-ray2.30A/B248-354[»]
4F50X-ray2.22A1-246[»]
4HYKX-ray2.80A1-354[»]
4NLGX-ray2.40A1-354[»]
ProteinModelPortaliQ4JB80.
SMRiQ4JB80.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4JB80.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 188UmuCUniRule annotationAdd BLAST186

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation
Contains 1 umuC domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04582. Archaea.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK04479.
OMAiVYSGRTK.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4JB80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVIFVDFDY FFAQVEEVLN PQYKGKPLVV CVYSGRTKTS GAVATANYEA
60 70 80 90 100
RKLGVKAGMP IIKAMQIAPS AIYVPMRKPI YEAFSNRIMN LLNKHADKIE
110 120 130 140 150
VASIDEAYLD VTNKVEGNFE NGIELARKIK QEILEKEKIT VTVGVAPNKI
160 170 180 190 200
LAKIIADKSK PNGLGVIRPT EVQDFLNELD IDEIPGIGSV LARRLNELGI
210 220 230 240 250
QKLRDILSKN YNELEKITGK AKALYLLKLA QNKYSEPVEN KSKIPHGRYL
260 270 280 290 300
TLPYNTRDVK VILPYLKKAI NEAYNKVNGI PMRITVIAIM EDLDILSKGK
310 320 330 340 350
KFKHGISIDN AYKVAEDLLR ELLVRDKRRN VRRIGVKLDN IIINKTNLSD

FFDI
Length:354
Mass (Da):39,986
Last modified:August 2, 2005 - v1
Checksum:i0EDDAEB0F30EAD35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79949.1.
RefSeqiWP_011277451.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79949; AAY79949; Saci_0554.
GeneIDi3474616.
KEGGisai:Saci_0554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79949.1.
RefSeqiWP_011277451.1. NC_007181.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BQ0X-ray2.60A1-354[»]
3BQ1X-ray2.70A1-354[»]
3BQ2X-ray2.70A1-354[»]
4F4WX-ray1.90A/B1-231[»]
4F4XX-ray2.05A1-231[»]
4F4YX-ray2.34A/B1-354[»]
4F4ZX-ray2.30A/B248-354[»]
4F50X-ray2.22A1-246[»]
4HYKX-ray2.80A1-354[»]
4NLGX-ray2.40A1-354[»]
ProteinModelPortaliQ4JB80.
SMRiQ4JB80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_0554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY79949; AAY79949; Saci_0554.
GeneIDi3474616.
KEGGisai:Saci_0554.

Phylogenomic databases

eggNOGiarCOG04582. Archaea.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK04479.
OMAiVYSGRTK.

Enzyme and pathway databases

BRENDAi2.7.7.7. 6160.

Miscellaneous databases

EvolutionaryTraceiQ4JB80.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO4_SULAC
AccessioniPrimary (citable) accession number: Q4JB80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: August 2, 2005
Last modified: November 30, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.