Q4JB78 (RTCA_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Saci_0556

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156435
Regions
	Nucleotide binding	282 – 285	4	ATP By similarity
Sites
	Active site	306	1	Tele-AMP-histidine intermediate By similarity
	Binding site	101	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JB78 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: FFF8965F6497118A	FASTA	335	36,732
	10 20 30 40 50 60 MIEIDGSFGE GGGQILRTSL TLSSLTKKPF RIYNIRANRP KPGLQRQHLT AVNAVKILTN 70 80 90 100 110 120 ATVKGDYVGS TELIFSPGDI QEKGDFVFDI GTAGSTTLIL QTILPLLLNR KLTVTIKGGT 130 140 150 160 170 180 DVPKSPSIDY IRLVFSKVLE RIGISFNVEL IKRGHYPEGG GEIRISNVRG EPQRFSITEF 190 200 210 220 230 240 GQLEGFVGIS HVSSLPVHIA DRQKSSAEKI LRRLKDKIDI RLDVREGEIS KGSGICLSAI 250 260 270 280 290 300 GKYGIIGADA LGERGKRAET VGEEAALKLL EELKTNAAFD SHMGDMLMLY ASLYQGEYTA 310 320 330 SKLTLHSITN EKVIRKFIDI KGEIKGSSPF LFRVQ

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY79951.1.
RefSeq	YP_255244.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4JB78.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3474618.
GenomeReviews	Gene locus Saci_0556 in contig CP000077_GR.
KEGG	sai:Saci_0556.
NMPDR	fig|330779.3.peg.1799.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG683539.
OMA	HYLALVW.
PhylomeDB	Q4JB78.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_0556-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_SULAC

Accession

Primary (citable) accession number: Q4JB78

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 2, 2005
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program