Q4JB01 (G1PDH_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Ordered Locus Names:	Saci_0640

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subunit structure	Homodimer By similarity. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.
Sequence caution	The sequence AAY80028.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_0000157352
Regions
	Nucleotide binding	97 – 101	5	NAD By similarity
	Nucleotide binding	119 – 122	4	NAD By similarity
Sites
	Metal binding	171	1	Zinc; catalytic By similarity
	Metal binding	251	1	Zinc; catalytic By similarity
	Metal binding	267	1	Zinc; catalytic By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	128	1	NAD By similarity
	Binding site	171	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JB01 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: 307FAC9F0BC9B20B	FASTA	351	38,584
	10 20 30 40 50 60 MEIKEHIINL PKHIYTGYGI LDNFRNYLQT LNLPQPFLVI TGPVIHQEIF SKRIEEHIKD 70 80 90 100 110 120 FKYEVVIVNK SDLSEAEKVE DIARQKGIKT ILGVGGGTVI DIAKFTAYKI DREFISIPTS 130 140 150 160 170 180 PSHDGITSPF AAIKGLGKPI SIKAKEPLAI ISDVEILASA PRRLINAGIG DTLGKITAVR 190 200 210 220 230 240 DWRLAHKLRG EYYGDYTASL ALMSARHALS CTKIINKDIR AGVRVLTEAL ISSGVAMGMA 250 260 270 280 290 300 GSTRPASGSE HLFAHAIELL YPNLGLHGEL VALGTIMMAY IHGINWRRIR RAMKKIGLPV 310 320 330 340 350 TSKQIGIPDE GIIKALTIAH SIRPERYTIL GDRGLTWESA EKIARETGVI S

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80028.1. Different initiation.
RefSeq	YP_255321.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4JB01.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3474123.
GenomeReviews	Gene locus Saci_0640 in contig CP000077_GR.
KEGG	sai:Saci_0640.
NMPDR	fig|330779.3.peg.2076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672951.
ProtClustDB	PRK00843.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_0640-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_SULAC

Accession

Primary (citable) accession number: Q4JB01

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	September 23, 2008
Last modified:	November 16, 2011
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program