Q4JAM1 (DNLI_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Saci_0788

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Magnesium By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	DNA ligase HAMAP MF_00407		PRO_0000059616
Sites
	Active site	260	1	N6-AMP-lysine intermediate By similarity
	Binding site	258	1	ATP By similarity
	Binding site	265	1	ATP By similarity
	Binding site	280	1	ATP By similarity
	Binding site	310	1	ATP By similarity
	Binding site	350	1	ATP By similarity
	Binding site	427	1	ATP By similarity
	Binding site	433	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4JAM1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: D8C6D946CF2D76A4	FASTA	598	67,576
	10 20 30 40 50 60 MEFKLIAEYF DRLEKISSRI QLTALLTDLL KKSDKDVIDK VIYITQGKLW PDFFEKPEIG 70 80 90 100 110 120 LGEKLMIKAL SVSTNIKEDE IEKQLRILGD LGEVAYKLKR TNSSQNVLSY LGSTKVTKLT 130 140 150 160 170 180 VEEVYESLSK IALSEGEGSR DMKIRSLAGL LKKADPLEAK YIIRFIDGRL RVGIGDATIM 190 200 210 220 230 240 DALANVFGGG TAARPLIERA YNLRADLGNI AKLLAENGIE TIKSIKPEVG IPIRPMLAER 250 260 270 280 290 300 LTDPSEILAK VGGKGIVDYK YDGERAQIHK KGEKVYIFSR RLENITRQYP DVVEYIKNSI 310 320 330 340 350 360 KSNEVILEGE IVAIDKESGE ILPFQNLMHR KRKNDIGEAI KEYPVNVYLF DLMYNDGEDY 370 380 390 400 410 420 TQKPLPDRRE KLEEIVTQND IVKVADHKYI DKVNELVEYF HQAISDGTEG VIVKSIGKDS 430 440 450 460 470 480 IYQAGARGFL WIKLKKDYQS EMADSVDLVI VGAFYGRGKR GGKLSSLLMA AYDPDKDTFY 490 500 510 520 530 540 TVCKVASGFS DAELEEVQKM LSEAKLDKKD PRVESEIQPD IWVKPKYVAE IIGAEITLSP 550 560 570 580 590 LHTCCKGVVS EEAGLSIRFP RFIRWRDDKS VEEATTPNEI LEMYQSRLKK KVEDITET

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80158.1.
RefSeq	YP_255451.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4JAM1.
SMR	Q4JAM1. Positions 1-590.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3473850.
GenomeReviews	Gene locus Saci_0788 in contig CP000077_GR.
KEGG	sai:Saci_0788.
NMPDR	fig|330779.3.peg.488.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG570821.
OMA	LAPAYEG.
PhylomeDB	Q4JAM1.
ProtClustDB	PRK01109.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_0788-MONOMER.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_SULAC

Accession

Primary (citable) accession number: Q4JAM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 2, 2005
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program